The effect of nitrification inhibitor on N2O, NO and N2 emissions under different soil moisture levels in a permanent grassland soil

Wu, Di; Cardenas, L. M.; Calvet, S.; Brüggemann, Nicolas; Loick, Nadine; Liu, Shurong; Bol, Roland

doi:10.1016/j.soilbio.2017.06.007

Cited by 81 publications

(36 citation statements)

References 59 publications

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“…The reduction of N 2 O emissions, together with the increase of nosZ abundance in the sandy CL suggests however that DMPP lowered NO 3 − availability below a soil specific treshold 35 , limiting the preferential reduction of NO 3 − over N 2 O. The results from the sandy CL confirm the proposed mechanism of N 2 O reduction via a shift in the N 2 :N 2 O ratio 26 , and show that DMPPs inhibitory effect on nitrification can limit the suppression of the N 2 O reductase, promoting complete denitrification to N 2 .…”

Section: Discussionsupporting

confidence: 57%

“…In the loam, NO 3 − levels were above those measured at the respective field site, which is also reflected in higher N 2 O d /(N 2 O d + N 2 ) ratios 11 . This phenomenon often occurs in incubation studies, where the absence of plant uptake, pre-incubation 33,34 , and the addition of glucose 26 increases NO 3 − levels in the soil. It is therefore important to consider N substrate availability when interpreting the effects of NIs on rate and N 2 O:N 2 partitioning of denitrification losses.…”

Section: Discussionmentioning

confidence: 99%

“…These results link the increase of nosZ abundance in response to DMPP in the sandy CL to a shift in the N 2 O d :N 2 ratio towards N 2 , based on direct measurements of N 2 and N 2 O d using the 15 N gas flux method. In contrast to previous incubation studies investigating N 2 O:N 2 partitioning in response to DMPP 26,27 , emissions of N 2 O and N 2 were quantified after incubation under atmospheric O 2 conditions and without adding an easily available C source to stimulate denitrification, as these conditions would have altered short-term N dynamics in response to DMPP. Importantly, the shift towards N 2 was not observed for the loam, where DMPP had a negligible effect on nitrification.…”

Section: Discussionmentioning

confidence: 99%

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Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Friedl

Scheer

Rowlings

et al. 2020

Sci Rep

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Nitrification inhibitors (NIs) have been shown to reduce emissions of the greenhouse gas nitrous oxide (n 2 O) from agricultural soils. However, their N 2 O reduction efficacy varies widely across different agroecosystems, and underlying mechanisms remain poorly understood. To investigate effects of the NI 3,4-dimethylpyrazole-phosphate (DMPP) on N-turnover from a pasture and a horticultural soil, we combined the quantification of N 2 and N 2 O emissions with 15 N tracing analysis and the quantification of the N 2 O-reductase gene (nosZ) in a soil microcosm study. Nitrogen fertilization suppressed nosZ abundance in both soils, showing that high nitrate availability and the preferential reduction of nitrate over N 2 O is responsible for large pulses of N 2 O after the fertilization of agricultural soils. DMPP attenuated this effect only in the horticultural soil, reducing nitrification while increasing nosZ abundance. DMPP reduced N 2 O emissions from the horticultural soil by >50% but did not affect overall n 2 + N 2 O losses, demonstrating the shift in the N 2 o:n 2 ratio towards N 2 as a key mechanism of N 2 O mitigation by NIs. Under non-limiting NO 3 − availability, the efficacy of NIs to mitigate N 2 O emissions therefore depends on their ability to reduce the suppression of the N 2 O reductase by high NO 3 − concentrations in the soil, enabling complete denitrification to N 2 .Agricultural soils have become the main source of anthropogenic nitrous oxide (N 2 O), a powerful greenhouse gas and the single most important substance depleting stratospheric ozone 1 . Delaying the conversion of ammonium (NH 4 + ) to nitrate (NO 3 − ), nitrification inhibitors (NIs) have been suggested as a means to reduce N 2 O emissions from agricultural soils. NIs demonstrated their efficacy across different cropping soils 2 , but results vary widely, and in particular in pasture soils the use of NIs had no or little effect on N 2 O emissions 3-5 . Despite a growing body of research on NIs, mechanisms and factors determining their efficacy to reduce N 2 O emission remain poorly understood 6 . The challenges to understand these mechanisms derive from the fact that N 2 O is formed via several different pathways in the soil matrix 7 , tightly coupled to different processes of N supply and consumption 8 . Critically, N 2 O can be further reduced to N 2 via the microbial-mediated process of denitrification, and the sole quantification of N 2 O as affected by NIs provides therefore only a limited insight into mechanisms of N 2 O mitigation using NIs.Microbial metabolic pathways can contribute via a wealth of different processes to N 2 O production and consumption, i.e. the reduction to N 2 in soils. Apart from abiotic processes, N 2 O formation can be categorized into www.nature.com/scientificreports www.nature.com/scientificreports/ nitrification-mediated pathways, denitrification and biotic formation of hybrid N 2 O 9 . Denitrification is generally assumed to be the main process contributing to overall N 2 O production from agricultu...

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Friedl

Scheer

Rowlings

et al. 2020

Sci Rep

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“…Further, the nitrite oxidizer bacteria Nitrobacter and Nitrospira have optimal growth under higher and lower nitrite supplies, respectively, which is linked to their ecological niches (Attard et al, 2010;Nowka et al, 2015). Nitrification is doubly implicated in N 2 O production, either directly or indirectly by producing NO 3 − as the basis for denitrification, and has been shown to be the main process involved in N 2 O emissions in some Brazilian sugarcane soils (Liu et al, 2016;Soares et al, 2016;Wu et al, 2017aWu et al, , 2017bLourenço et al, 2018aLourenço et al, , 2018b. The addition of nitrification inhibitors with nitrogen fertilizers is currently being explored as a sustainable management practice in Brazilian sugarcane Soares et al, 2015Soares et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Nitrification inhibitors effectively target N₂O‐producing Nitrosospira spp. in tropical soil

Cassman

Soares

Pijl

et al. 2019

Environmental Microbiology

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SummaryThe nitrification inhibitors (NIs) 3,4‐dimethylpyrazole (DMPP) and dicyandiamide (DCD) can effectively reduce N2O emissions; however, which species are targeted and the effect of these NIs on the microbial nitrifier community is still unclear. Here, we identified the ammonia oxidizing bacteria (AOB) species linked to N2O emissions and evaluated the effects of urea and urea with DCD and DMPP on the nitrifying community in a 258 day field experiment under sugarcane. Using an amoA AOB amplicon sequencing approach and mining a previous dataset of 16S rRNA sequences, we characterized the most likely N2O‐producing AOB as a Nitrosospira spp. and identified Nitrosospira (AOB), Nitrososphaera (archaeal ammonia oxidizer) and Nitrospira (nitrite‐oxidizer) as the most abundant, present nitrifiers. The fertilizer treatments had no effect on the alpha and beta diversities of the AOB communities. Interestingly, we found three clusters of co‐varying variables with nitrifier operational taxonomic units (OTUs): the N2O‐producing AOB Nitrosospira with N2O, NO3 −, NH4 +, water‐filled pore space (WFPS) and pH; AOA Nitrososphaera with NO3 −, NH4 + and pH; and AOA Nitrososphaera and NOB Nitrospira with NH4 +, which suggests different drivers. These results support the co‐occurrence of non‐N2O‐producing Nitrososphaera and Nitrospira in the unfertilized soils and the promotion of N2O‐producing Nitrosospira under urea fertilization. Further, we suggest that DMPP is a more effective NI than DCD in tropical soil under sugarcane.

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“…Parameters of the soil structure such as air permeability, aggregate size distribution, and size and design of pore space seem to play an important role. On the one hand, they have a direct influence on the movement of the gases via mass flow and diffusion in the soil in a variety of ways and thus also the emission of greenhouse gases [33][34][35][36][37][38][39][40][41] . On the other hand, they act indirectly by controlling the availability of oxygen in the soil, which in turn influences the processes of CO 2 and N 2 O formation and N 2 O consumption in many ways [42][43][44][45] .…”

Section: Introductionmentioning

confidence: 99%

Microplastic fibers affect dynamics and intensity of CO₂and N₂O fluxes from soil differently

Rillig

Hoffmann

Lehmann

et al. 2020

Preprint

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Microplastics may affect soil ecosystem functioning in critical ways, with previously documented effects including changes in soil structure and water dynamics; this suggests that microbial populations and the processes they mediate could also be affected. Given the importance for global carbon and nitrogen cycle and greenhouse warming potential, we here experimentally examined potential effects of plastic microfiber additions on CO2 and N2O greenhouse gas fluxes. We carried out a fully factorial laboratory experiment with the factors presence of microplastic fibers (0.4% w/w) and addition of urea fertilizer (100 mg N kg−1). The conditions in an intensively N-fertilized arable soil were simulated by adding biogas digestate at the beginning of the incubation to all samples. We continuously monitored CO2 and N2O emissions from soil before and after urea application using a custom-built flow-through steady-state system, and we assessed soil properties, including soil structure. Microplastics affected soil properties, notably increasing soil aggregate water-stability and pneumatic conductivity, and caused changes in the dynamics and overall level of emission of both gases, but in opposite directions: overall fluxes of CO2 were increased by microplastic presence, whereas N2O emission were decreased, a pattern that was intensified following urea addition. This divergent response is explained by effects of microplastic on soil structure, with the increased air permeability likely improving O2 supply: this will have stimulated CO2 production, since mineralization benefits from better aeration. Increased O2 would at the same time have inhibited denitrification, a process contributing to N2O emissions, thus likely explaining the decrease in the latter. Our results clearly suggest that microplastic consequences for greenhouse gas emissions should become an integral part of future impact assessments, and that to understand such responses, soil structure should be assessed.

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The effect of nitrification inhibitor on N2O, NO and N2 emissions under different soil moisture levels in a permanent grassland soil

Cited by 81 publications

References 59 publications

Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Nitrification inhibitors effectively target N₂O‐producing Nitrosospira spp. in tropical soil

Microplastic fibers affect dynamics and intensity of CO₂and N₂O fluxes from soil differently

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The effect of nitrification inhibitor on N2O, NO and N2 emissions under different soil moisture levels in a permanent grassland soil

Cited by 81 publications

References 59 publications

Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N-turnover, the N2O reductase-gene nosZ and N2O:N2 partitioning from agricultural soils

Nitrification inhibitors effectively target N2O‐producing Nitrosospira spp. in tropical soil

Microplastic fibers affect dynamics and intensity of CO2and N2O fluxes from soil differently

Contact Info

Product

Resources

About

Nitrification inhibitors effectively target N₂O‐producing Nitrosospira spp. in tropical soil

Microplastic fibers affect dynamics and intensity of CO₂and N₂O fluxes from soil differently