The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction

Ortega-Morales, Luis D.; Alcantar-Rodriguez, Alicia; Espejel, Maria C.; Medrano, Alfredo Soriano

doi:10.4067/s0719-81322019000200073

Cited by 4 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results on membrane fluidity support our hypothesis that high doses (0.002 and 0.0035 mol/L) of MLT significantly reduce the percentage of sperm with hyper-fluid membranes after freeze-thawing. However, similar results were obtained in dog sperm by the mere effect of prefreeze cooling (Ortega-Morales et al 2019). We argue that because the difference in the percentage of hyper-fluid membranes due to MLT is larger than that of cooling to -5 °C alone, there is an additive effect of MLT and cooling.…”

Section: Discussionsupporting

confidence: 79%

“…In a similar study, using a different breed of dogs (Belgian and German Shepherds), cooling reduced the percentage of hyper-fluid membranes in comparison to the usual cooling to +5 °C (Ortega-Morales et al 2019). These observations support the proposed hypothesis that the cooling of spermatozoa to subzero temperatures favours sperm plasma membrane reorganisation after the lipid phase transition takes place, thus avoiding an excessive increase in membrane fluidity (Watson 1995;Holt 2000).…”

Section: Discussionmentioning

confidence: 90%

“…Many approaches have been used to reduce cryodamage. We have tested the effect of different pre-freeze cooling temperatures on boar (Garzon-Perez et al 2010), horse (Contreras-Mendez and Medrano 2016), and dog spermatozoa (Alcantar-Rodriguez and Morales-Ortega et al 2019). Another approach we have tested is the use of melatonin (MLT) as an antioxidant in cooling/freezing media for the conservation of ram (Cano-Suarez et al 2013), goat buck (Moazami et al 2014), and boar spermatozoa (Martinez-Hernandez et al 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

Martinez-Rodriguez

Carbajal

Martinez-De-Anda

et al. 2020

Reproduction and Fertility

Self Cite

View full text Add to dashboard Cite

Cryopreservation compromises sperm fertilising capacity due to a series of alterations in sperm structure and physiology; use of antioxidants such as melatonin, added to freezing media, may help to reduce sperm cryoinjury. To test the effect of melatonin on Bulldog [Canis lupus familiaris] sperm cryosurvival, spermatozoa were diluted in a standard freezing medium, cooled to 5°C, and added more freezing medium to obtain 200 x 106 cells/mL, and 5% glycerol. Diluted spermatozoa were added melatonin (0.0, 0.0005, 0.002, and 0.0035 mol/L), and packaged in 0.25 mL straws which were further cooled to -5°C before freezing in liquid nitrogen. Thawing was carried out at 70°C for 5 sec, and then spermatozoa (at 37°C) were assessed for progressive motility, viability, plasma membrane integrity, acrosome integrity, capacitation status, and plasma membrane fluidity; data was analysed by ANOVA to detect differences between melatonin doses. There were differences (P<0.05) in the percentage of sperm having hyper-fluid membranes, intact acrosome, capacitated acrosome-intact, and acrosome reacted, being the values for high (0.002 and 0.0035 mol/L) better than for low (0.0 and 0.0005 mol/L) melatonin doses. In conclusion, 0.002 and 0.0035 mol/L melatonin improved cryosurvival of sperm from Bulldog males.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

Martinez-Rodriguez

Carbajal

Martinez-De-Anda

et al. 2020

Reproduction and Fertility

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the reduced of temperature during chilling processing which preserved at 5 °C or less inflicts sub-lethal damage to spermatozoa, compromising sperm quality and the success of artificial breeding as described by previous studies (Mohamed et al, 2020 andBustani andBaiee 2021). The earlier studies have concluded effect of chilling temperature on the sperm motility in both general and progressive motile of rat (Varisli et al, 2009), bull (Januskauskas et al, 1999), rabbit (Rosato et al, 2011), human (Verheyen et al, 1993) and, ram (Kulaksiz et al, 2012) which illustrated the temperature related in the organization of structural components of the plasma membrane have profound effects upon its properties, associated with changes in permeability, changes in membrane fluidity (Ortega et al, 2019), dis-regulation of intracellular Ca+' influx (Yeste et al, 2018) and changes in enzyme activity (Palhares et al, 2021) finally on the ability of sperm capacitation. From the Figure (1 and 2) the result showed the improved effect of Prunus cerasus (G1) on the sperm motility (general and progressive).…”

Section: Discussionmentioning

confidence: 99%

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

AbdUlAmeer¹,

Zaid²

2022

ijhs

View full text Add to dashboard Cite

This study was designed in order to investigate and determine the effect of black current (Prunus cerasus) extract on semen general and progressive motility of the rams. Twelve mature healthy rams was used in this study aged between 2-3 years, kept in the animal farm of the College of Veterinary Medicine, Al-Kufa University. Semen ejaculates was collected using electroejaculator methods once weekly a total of 36 ejaculates were collected among the period of the study. The black current (Prunus cerasus) extract was prepared by using maceration and added to the semen in five doses groups (0.25 gm/ml as G1, 0.5 gm/ml as G2, 1 gm/ml as G3, 2 gm/ml as G4, 4 gm/ml as G5) and the positive control group left without any addition. The treatment samples were evaluated under cooling for five consecutive days, the general and progressive motility were analysis using Computer Assisted Sperm Analysis (CASA). The results of the study clearly indicated that there were a significant differences (P<0.05) in G1 and G2 than other groups in five days of examination, with the preference to the G1 group.

show abstract

“…Notou-se, ainda na Tabela 3, que o grupo do ácido palmítico (G2) apresentou a melhor porcentagem de espermatozoides com MP íntegra (45,60%) se sobressaindo do G3 (39,80%), que também foi melhor que o controle (28,4%). A composição lipídica da membrana plasmática dos espermatozoides está diretamente relacionada à MOT e à fluidez dessa membrana [22].…”

Section: Discussionunclassified

Effect of Addition Palmitic Acid and Vitamin E to the Tris-Egg Yolk Diluter in Canine Semen Cryopreservation

Filho

Evangelista

Barros

et al. 2021

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Canine sperm is a very delicate cell that is quite susceptible to oxidative stress since the cytoplasm is restricted and features little antioxidant reserves. Furthermore, the sperm membrane has some polyunsaturated fatty acids sensitive to lipid peroxidation, which makes it important to addition antioxidant substances to the diluter aiming at decreasing such stress to the sperm cell, particularly during seminal cryopreservation. Several antioxidants have been used in this process in some domestic animal’s species, however, the use of palmitic acid has been little reported in works on cryopreservation of semen of the canine species. Hence, this study aimed to assess the effect of addition antioxidants palmitic acid and vitamin E to the Tris-egg yolk diluter on the semen quality of dogs after thawing.Materials, Methods & Results: Samples were collected from the ejaculates of 4 adult dogs, apparently healthy, of the American Pit Bull Terrier breed of kennels in the city of Teresina, PI, places where the pre-freezing procedures of the dog’s semen were performed. The samples were diluted in Tris citric acid fructose (3.28 g Tris-hydroxymethyl-aminomethane, 1.78 g citric acid monohydrate and 1.25 g D-fructose), dissolved in 100 mL distilled water, and added 20% egg yolk and 6% glycerol, at the concentration of 100x106 sptz/mL. The semen samples were divided into 3 mL aliquots to form 3 experimental groups: G1 - Only Tris-egg yolk (Control group); G2 - Tris-egg yolk + 100 µM palmitic acid; and G3 - Tris-egg yolk + 116 µM vitamin E. Semen was collected weekly over a period of little over 2 months. After thawing, thermorresistance test (TTR) was carried out at 0, 30, 60, and 90 min to assess spermatics motility and vigor, in addition to analysis of integrity of plasma membrane, acrosomal membrane and mitochondrial activity of the sperm, using fluorescent probes. These assessments were performed out at the Animal Reproduction Biotechnology Laboratory (LBRA/UFPI). In the TTR, G2 and G3 didn´t exhibit significant results for spermatics motility or vigor when compared with the control group. The palmitic acid and vitamin E also had no significant effects on the parameters of acrosomal membrane integrity or mitochondrial activity. However, sperm cryopreserved with the addition of palmitic acid exhibited significant differences for plasma membrane integrity, providing greater protection to the sperm cells in G2.Discussion: The palmitic acid is one of the most saturated fatty acids in human semen, with reports of great proportions also in the seminal plasma of dogs. Its main role is to protect the plasma membrane from external damage, improving viability and fertility of the sperm after cryopreservation. Data is scarce in the literature on the composition of fatty acids in canine semen and regarding the use of palmitic acid as a seminal antioxidant in that species, which grants further studies aiming to investigate such valuable information for canine reproduction. It is concluded that addition palmitic acid at 100 µM concentration to the Tris-egg yolk diluter was able to preserve the integrity of the plasma membrane during the process of cryopreservation of canine semen.Keywords: dog, semen, antioxidants, cryopreservation.Descritores: cão, sêmen, antioxidantes, criopreservação.

show abstract

The effect of non-traditional cooling on dog sperm cryosurvival and ability to perform the acrosome reaction

Cited by 4 publications

References 22 publications

Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

Melatonin added to freezing diluent improves canine (Bulldog) sperm cryosurvival

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

Effect of Addition Palmitic Acid and Vitamin E to the Tris-Egg Yolk Diluter in Canine Semen Cryopreservation

Contact Info

Product

Resources

About