The effect of nutrient limitation on hydrogen production by batch cultures of Escherichia coli

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

“…Similarly, a study where sucrose was varied at a constant ammonium concentration showed that conversion to hydrogen was more efficient at lower substrate loadings [5] . Another study was conducted by Bisaillon et al [6] to investigate some limiting factors in microbial hydrogen fermentation by different strains of E. coli. They found that limitation of phosphate or sulfate was without great effect.…”

Ethanol and Hydrogen Production with Thermophilic Bactera from Sugars and Complex Biomass

“…Logically, inactivation of H 2 oxidation would increase H 2 yields. This has been shown for E. coli where elimination of hyd1 and hyd2 led to a 37% increase in H 2 yield compared to the wild type strain (Bisaillon et al, 2006). Studies on metabolically engineering Clostridia to increase H 2 production have been published.…”

Microbiological Methods of Hydrogen Generation

“…Genes responsible for nickel-iron hydrogenases (hydrogenase I and II) coded by hya and hyb operons were found in the E. coli genom as well. It was found that elimination of these enzymes by genetic modification can result with almost 35% higher production of hydrogen (Hallenback, 2009, Bisaillon, 2006, Turcot, 2008. Other profits originating from genetic engineering are related to deactivation of enzymes responsible for transformations of glucose into lactic, succinic and fumaric acids.…”