The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality

Leroy, Jo; Hoeck, Veerle Van; Clemente, María; Rizos, Dimitrios; Gutiérrez‐Adán, Alfonso; Soom, Ann Van; Uytterhoeven, Marc; Bols, P.E.J.

doi:10.1093/humrep/dep420

Cited by 78 publications

(32 citation statements)

References 74 publications

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“…Because elevated NEFA concentrations can be cytotoxic, Van Hoeck et al (2011) went on to demonstrate that exposure to high concentrations of NEFAs, in particular oleic acid, palmitic acid and stearic acid, during oocyte maturation reduced subsequent embryo quality, measured in terms of cell number, gene expression and apoptotic cell ratio. Similar adverse effects were produced by culturing bovine zygotes with serum derived from heifers fed a high-fat diet supplemented with palm oil (Leroy et al 2010). Together, these experiments suggest that a period of extreme NEB experienced after calving, which will inevitably be accompanied by elevated circulating NEFA concentrations, is likely to impair oocyte quality.…”

Section: Effects Of Lipid Metabolism On the Reproductive Tract Ovarymentioning

confidence: 72%

Associations between lipid metabolism and fertility in the dairy cow

Wathes

Clempson

Pollott

2013

Reprod. Fertil. Dev.

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Abstract. Dairy cows mobilise body tissues to support milk production and, because glucose supplies are limited, lipids are used preferentially for energy production. Lipogenic activity is switched off and lipolytic mechanisms in adipose tissue increase through changes in the expression of several key enzymes. This results in a loss of body condition, together with high circulating concentrations of non-esterified fatty acids. Changes in the synthesis, secretion and signalling pathways of somatotrophic hormones (insulin, growth hormone, insulin-like growth factor 1) and adipokines (e.g. leptin) are central to the regulation of these processes. A high reliance on fatty acids as an energy source in the peripartum period causes oxidative damage to mitochondria in metabolically active tissues, including the liver and reproductive tract. The expression of genes involved in insulin resistance (PDK4, AHSG) is increased, together with expression of TIEG1, a transcription factor that can induce apoptosis via the mitochondrial pathway. Polymorphisms in TFAM and UCP2, two autosomal mitochondrial genes, have been associated with longevity in dairy cows. Polymorphisms in many other genes that affect lipid metabolism also show some associations with fertility traits. These include DGAT1, SCD1, DECR1, CRH, CBFA2T1, GH, LEP and NPY. Excess lipid accumulation in oocytes and the regenerating endometrium reduces fertility via reductions in embryo survival and increased inflammatory changes, respectively.

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Section: Effects Of Lipid Metabolism On the Reproductive Tract Ovarymentioning

confidence: 72%

Associations between lipid metabolism and fertility in the dairy cow

Wathes

Clempson

Pollott

2013

Reprod. Fertil. Dev.

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“…In a recent study, Wakefield et al (2007) suggested that contrary to the possible beneficial effects, supplementing a ration with n-3 PUFAs during the period just before or immediately after conception, may reduce normal development of the embryo, since this seems to disturb mitochondrial metabolism. Leroy et al (2010) cultivated bovine zygotes in media supplemented with serum from heifers fed rations with a high content of lipids protected from rumen biohydrogenation and observed a lower production of blastocysts compared to a control treatment, noting that in the first there was a greater expression of genes related to apoptosis. Even when in vivo and in vitro studies have shown a better embryo development with rations supplemented with lipids, results are not consistent and it is important to ascertain in particular, which fatty acids are the most beneficial for embryo survival .…”

Section: Lipids Their Effect On Embryo Developmentmentioning

confidence: 99%

Effect of Fatty Acids on Reproductive Performance of Ruminants

Herrera-Camacho¹,

Soberano-Martínez²,

Duran³

et al. 2011

Artificial Insemination in Farm Animals

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“…A similar approach was previously used [41] to study the effect of induced nutritrional hyperlipidaemia on in vitro bovine embryos;…”

Section: Discussionmentioning

confidence: 99%

Effect of blood plasma collected after adrenocorticotropic hormone administration during the preovulatory period in the sow on oocyte in vitro maturation

González

2013

Theriogenology

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Reproduction may be affected by stressful events changing the female endocrine or metabolic profile. An altered environment during oocyte development could influence the delicate process of oocyte maturation. Here, the effect of simulated stress by media supplementation with blood plasma from sows after adrenocorticotropic hormone (ACTH) administration during the preovulatory period was assessed. Oocytes were matured for 46 h in the presence of plasma from ACTH-treated sows, or plasma from NaCl-treated control sows, or medium without plasma (BSA group). The plasma used had been collected at 36 h and 12 h (± 2 h) before ovulation (for the first 24 h + last 22 h of maturation, respectively).Subsequent fertilisation and embryo development were evaluated. Actin cytoskeleton and mitochondrial patterns were studied by confocal microscopy both in the oocytes and the resulting blastocysts. Nuclear maturation did not differ between treatments. Subtle differences were observed in the actin microfilaments in oocytes; however, mitochondrial patterns were associated with the treatment (P<0.001). These differences in mitochondrial patterns were not reflected by in vitro outcomes, which were similar in all groups. In conclusion, an altered hormonal environment provided by a brief exposure to plasma from ACTH-treated sows during in vitro oocyte maturation, could induce alterations in actin cytoskeleton and mitochondrial patterns in oocytes. However, these changes might not hamper the subsequent in vitro embryo development.[217 words]

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The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality

Abstract: This combined in vivo and in vitro model indicates that the exposure of preimplantation embryos to hyperlipidaemic conditions may result in reduced embryo quality and developmental potential, possibly resulting in poorer fertility.

Cited by 78 publications

References 74 publications

Associations between lipid metabolism and fertility in the dairy cow

Associations between lipid metabolism and fertility in the dairy cow

Effect of Fatty Acids on Reproductive Performance of Ruminants

Effect of blood plasma collected after adrenocorticotropic hormone administration during the preovulatory period in the sow on oocyte in vitro maturation

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