The effect of operating conditions on the optically induced electrokinetic (OEK)-based manipulation of magnetic microbeads in a microfluidic system

The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications

“…ODEP chips were mainly constructed of a top conductive electrode of ITO/glass, a microchannel, and a bottom photoconductive semiconductor layer of a-Si:H/ITO/glass. The detailed process of the whole system is shown in our previous publication [ 19 ]. By following a previous design, the same structure of four stacked layers was fabricated by replacing a microchannel design, a new resource of top ITO/glass and a-Si:H/ITO/glass, which were fabricated by InnoLux Corporation (ZMI, Taiwan).…”

“…For a high efficiency and low cost of detection, the total volume of biological samples and reagents need to be reduced in real applications. There are new technologies engaged to satisfy these requirements, including the use of microfluidic design [ 15 , 16 , 17 ] and magnetic beads [ 18 , 19 , 20 ]. One of the most promising platforms is a digital ELISA method (e.g., called the single-molecule array (Simoa) proposed by David Walt’s group 2).…”

“…Among these results, the limitation of simultaneous multiple sensing by means of magnetic beads still remains an issue. To keep the advantages of magnetic separation and explore further possibilities with affordable price and easy operation, we would like to explore the behavior of antibody-functionalized magnetic beads manipulated in a microfluidic channel by the optically induced electrokinetic (OEK), such as light-actuated AC electroosmosis (LACE) and optically induced dielectrodphoresis (ODEP) under certain operation conditions in the previous literature [ 19 ]. With the photosensitive semiconductor and specially designed illuminated light patterns, ODEP-based manipulation, as with the similar performance of dielectrophoresis (DEP) [ 31 ], can be easily achieved to control magnetic beads via a more flexible arrangement compared to conventional methods such as DEP and acoustofluidic methods [ 32 ].…”

Optoelectronic tweezers: a versatile toolbox for nano-/micro-manipulation

“…Similar results were also reported for TMP assembled by OET but preserved via photopolymerization. 127 Apart from polystyrene microbeads, OET was also used to manipulate magnetic microbeads and study their electrokinetic behaviors, 150,151 which is useful for the concentration and purification of biological substances that can attach to magnetic microbeads. OET can also be applied to manipulate gas and liquid phases, such as gas bubbles, 51 waterin-oil and oil-in-water droplets.…”

The electrodynamics of rod‐like microparticles based on optically induced dielectrophoresis

“…The optically induced dielectrophoresis (ODEP) technology is combined with a virtual electrode defined by light irradiating on the photoconductive materials to generate a nonuniform electric field to realize the manipulation of cells [29,30]. Compared to traditional electrophoretic techniques, ODEP discards the preparation of expensive metal electrode structures, and it uses advanced digital micromirror device to write the designed electrode patterns into a photoconductive material to mimic physical electrode [31,32], so called optical virtual electrode. Based on the real-time reconfigured optical virtual electrode, flexible manipulation mode can be achieved, which is not available in traditional electrophoretic techniques.…”