The Effect of Osteogenic Protein-1 in an In Vivo Physeal Injury Model

Johnstone, Edward W.; Mcarthur, Maggie; Solly, P. B.; Higginson, Kerry; Byers, Sharon; Foster, Bruce K.

doi:10.1097/00003086-200202000-00028

Cited by 11 publications

(11 citation statements)

References 16 publications

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“…This dosage was the same as that used in the study by Johnstone et al [17], which reported an increase in growth plate height adjacent to the defect. This dosage is within the range of that used in other in vivo studies to regenerate articular and thyroid cartilages, doses of which have ranged from 11 pg to 500 pg f4, 16,18,24].…”

Section: Discussionmentioning

confidence: 99%

“…As type I collagen is a natural component of bone, its degradation and degradation products can be mediated by physiological means [31]. The type I collagen scaffold is not suitable for growth plate defects, as it induces bone formation within the defect site without the presence of rhOP-1 [17]. A scaffold that resembles growth plate cartilage matrix (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…A small cohort of animals was used, as these experiments were to extend previous results obtained in this model [17]. All procedures were sanctioned by the institutional animal ethics committee and performed under sterile conditions.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…Recently, rhOP-1 has been inserted into growth plate defects in sheep to promote regeneration of the growth plate cartilage adjacent to the introduced defect [17]. The results showed an increase of the growth plate width until the expansion of the growth plate cartilage ceased due to the formation of a bone bridge within the defect.…”

Section: Introductionmentioning

confidence: 99%

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The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

et al. 2005

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Injuries to the growth plate in children can result in bone bridge formation, which ultimately lead to limb length and angular deformities. The histological and molecular changes associated with growth plate repair following the Langenskiold procedure, a surgical technique used to remove impeding bone bridges, in conjunction with administration of recombinant human osteogenic protein-1 (rhOP-1) were examined using a sheep model. Following treatment with rhOP-1 there was an increase in the'height of the growth plate immediately adjacent to the defect compared to untreated animals. The expression of type I collagen, osteopontin and decorin were observed in the growth plate adjacent to the defect in the untreated animals at day 56, but this response was accelerated in the rhOP-1 treated animals, with these molecules seen as early as day 7. Therefore, treatment with rhOP-1 initiated a complex response that was both chondrogenic and osteogenic in nature.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Surgical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

et al. 2005

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“…The effect of osteogenic protein-1 (OP-1), also known as BMP-7, on the growth plate has also been studied in two different studies; Johnstone et al [56] reported local overgrowth of the physeal cartilage in response to OP-1.…”

Section: Pathogenesis Of Physeal Bar Formationmentioning

confidence: 99%

The normal and fractured physis: an anatomic and physiologic overview

Hosseinzadeh

Milbrandt

2016

Journal of Pediatric Orthopaedics B

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The growth plate (physis) is responsible for enabling and regulating longitudinal growth of upper and lower limbs. This regulation occurs through interaction of the cells in the growth plate with systemic and locally produced factors. This complex interaction leads to precisely controlled changes in chondrocyte size, receptors, and matrix, which ultimately result in endochondral bone formation. With advances in cellular and molecular biology, our knowledge about these complex interactions has increased significantly over the past decade. Deficiency of any of the regulating factors or physeal injury during childhood can alter this well-orchestrated sequence of events and lead to abnormalities in growth. This review highlights the histology of the normal physis, including recent findings at the cellular and molecular levels, mechanics and mechanobiology of the growth plate, pathologies that can affect the physis, and treatment options, including interposition materials.

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Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells

McCarty

Gronthos

Zannettino

et al. 2008

Journal Cellular Physiology

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132

126

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Since discovery, significant interest has been generated in the potential application of mesenchymal stem cells or multipotential stromal cells (MSC) for tissue regeneration and repair, due to their proliferative and multipotential capabilities. Although the sheep is often used as a large animal model for translating potential therapies for musculoskeletal injury and repair, the characteristics of MSC from ovine bone marrow have been inadequately described. Histological and gene expression studies have previously shown that ovine MSC share similar properties with human and rodents MSC, including their capacity for clonogenic growth and multiple stromal lineage differentiation. In the present study, ovine bone marrow derived MSCs positively express cell surface markers associated with MSC such as CD29, CD44 and CD166, and lacked expression of CD14, CD31 and CD45. Under serum-deprived conditions, proliferation of MSC occurred in response to EGF, PDGF, FGF-2, IGF-1 and most significantly TGF-alpha. While subcutaneous transplantation of ovine MSC in association with a ceramic HA/TCP carrier into immunocomprimised mice resulted in ectopic osteogenesis, adipogenesis and haematopoietic-support activity, transplantation of these cells within a gelatin sponge displayed partial chondrogenesis. The comprehensive characterisation of ovine MSC described herein provides important information for future translational studies involving ovine MSC.

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The Effect of Osteogenic Protein-1 in an In Vivo Physeal Injury Model

Cited by 11 publications

References 16 publications

The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

The effect of recombinant human osteogenic protein-1 on growth plate repair in a sheep model

The normal and fractured physis: an anatomic and physiologic overview

Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells

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