The effect of pentaghrelin on amylase release from the rat and porcine dispersed pancreatic acinar cells in vitro

Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation

“…The intra-and inter-assay variations for the trypsin activity determination were 2.8 and 3.2%, respectively. Amylase (EC 3.2.1.1) activity was measured according to Bernfeld (1955) with minor modification by Jankowska et al (2007). Lipase (EC 3.1.1.3) activity was determined according to the method described by Furukawa et al (1982) with absorbance of samples read at a wave length of 412 nm after 10 and 20 min (Quan-tiChrom™ Lipase Assay Kit, Cat.…”

“…Dispersed acinar cells were obtained from rat pancreas by collagenase digestion according to the method proposed by Jankowska et al (2007). In total 14 male Wistar rats were euthanatized by CO 2 inhalation.…”

Effects of dietary leucine and phenylalanine on gastrointestinal development and small intestinal enzyme activities in milk-fed holstein dairy calves

“…Different dietary nutrient levels can affect the gastrointestinal development in calves [1,2,3], with diets with suitably high protein levels shown to promote gastrointestinal development in calves [4,5]. Leucine, as a functional amino acid [6], can activate the mammalian target of rapamycin pathway to regulate protein synthesis and catabolism in the animal body [7,8,9,10] and promote the development of the gastrointestinal tract in animals [11]; phenylalanine is an aromatic amino acid and can promote the secretion of cholecystokinin (CCK) through calcium-sensing receptors [12,13]. CCK can stimulate the pancreas to synthesize pancreatic amylase, trypsin and trypsinogen, and it can further stimulate the release of pancreatic enzymes and enhance pancreatic enzyme activity [14].…”