The effect of photopolymerization on stem cells embedded in hydrogels

Fedorovich, Natalja E.; Oudshoorn, Marion H.M.; Geemen, Daphne van; Hennink, Wim E.; Alblas, Jacqueline; Dhert, Wouter J.A.

doi:10.1016/j.biomaterials.2008.09.037

Cited by 378 publications

(289 citation statements)

References 54 publications

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“…This also allows users to capture stiffness when 3D geometry is unnecessary to answer the biological question at hand. Others have developed 2D hydrogel platforms, 7,20 which produce perfectly flat hydrogels, but they are complicated to fabricate 7 [35][36][37] and it was used for 2D PEG-PC hydrogels when they were first developed for coverslips. 34 Irgacure is not a very efficient photoinitiator 26 because it does not absorb light readily at 365 nm, 35 but this wavelength is used in other platforms that include cell encapsulation to limit damage to the cells.…”

Section: Discussionmentioning

confidence: 99%

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Brooks

Jansen

Gencoglu

et al. 2018

ACS Biomater. Sci. Eng.

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Tunable biomaterials that mimic selected features of the extracellular matrix (ECM), such as its stiffness, protein composition, and dimensionality, are increasingly popular for studying how cells sense and respond to ECM cues. In the field, there exists a significant trade-off for how complex and how well these biomaterials represent the in vivo microenvironment, versus how easy they are to make and how adaptable they are to automated fabrication techniques. To address this need to integrate more complex biomaterials design with high-throughput screening approaches, we present several methods to fabricate synthetic biomaterials in 96-well plates and demonstrate that they can be adapted to semiautomated liquid handling robotics. These platforms include 1) glass bottom plates with covalently attached ECM proteins, and 2) hydrogels with tunable stiffness and protein composition with either cells seeded on the surface, or 3) laden within the three-dimensional hydrogel matrix. This study includes proof-of-concept results demonstrating control over breast cancer cell line phenotypes via these ECM cues in a semi-automated fashion. We foresee the use of these methods as a mechanism to bridge the gap between high-throughput cell-matrix screening and engineered ECM-mimicking biomaterials.

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Section: Discussionmentioning

confidence: 99%

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Brooks

Jansen

Gencoglu

et al. 2018

ACS Biomater. Sci. Eng.

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“…Microfluidics has previously been used with alginate to encapsulate cells with high viability [23][24][25] and more recently with thiol-ene click reactions with PEG. 17 UV polymerisation is a common method for encapsulating cells in bulk gels and has been used with many cell types including chondrocytes 49 and mesenchymal stem cells 50 with high viability. Additionally, L929 fibroblasts that were encapsulated in the same PVA microspheres produced by a UV photopolymerisation and electrospray method also had >90% viability at 24 h. 8 Heparin has been incorporated into bulk hydrogels for the purpose of sustained growth factor release and presentation to cells and was shown to have beneficial effects on viability.…”

Section: Cell Encapsulationmentioning

confidence: 99%

Poly(vinyl alcohol)-heparin biosynthetic microspheres produced by microfluidics and ultraviolet photopolymerisation

et al. 2013

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Biosynthetic microspheres have the potential to address some of the limitations in cell microencapsulation; however, the generation of biosynthetic hydrogel microspheres has not been investigated or applied to cell encapsulation. Droplet microfluidics has the potential to produce more uniform microspheres under conditions compatible with cell encapsulation. Therefore, the aim of this study was to understand the effect of process parameters on biosynthetic microsphere formation, size, and morphology with a co-flow microfluidic method. Poly(vinyl alcohol) (PVA), a synthetic hydrogel and heparin, a glycosaminoglycan were chosen as the hydrogels for this study. A capillary-based microfluidic droplet generation device was used, and by varying the flow rates of both the polymer and oil phases, the viscosity of the continuous oil phase, and the interfacial surface tension, monodisperse spheres were produced from $200 to 800 lm. The size and morphology were unaffected by the addition of heparin. The modulus of spheres was 397 and 335 kPa for PVA and PVA/heparin, respectively, and this was not different from the bulk gel modulus (312 and 365 for PVA and PVA/heparin, respectively). Mammalian cells encapsulated in the spheres had over 90% viability after 24 h in both PVA and PVA/heparin microspheres. After 28 days, viability was still over 90% for PVA-heparin spheres and was significantly higher than in PVA only spheres. The use of biosynthetic hydrogels with microfluidic and UV polymerisation methods offers an improved approach to long-term cell encapsulation. V C 2013 AIP Publishing LLC. [http://dx

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“…Gelation can be controlled by changing pH or temperature or addition of additives (11). Photopolymerization is an attractive technique, because the gelation occurs rapidly, under physiological temperature, with minimal heat production and controllable space and time characteristics (12). Degradation Gel matrix may be reabsorbed by simple dissolution, hydrolysis, or enzymatic hydrolysis (10).…”

Section: Gelation Timementioning

confidence: 99%

“…Hydrogel-forming polymers can be tailored to exhibit biochemical, cellular, and physical stimuli that guide cellular processes, including cell migration, proliferation, and differentiation (Table 1) (12). Biologically active additives have been added to the hydrogels, including bone morphogenic protein (BMP) and fibroblast growth factor (FGF) (16,17).…”

Section: Modification With Biologically Active Additivesmentioning

confidence: 99%

The use of hydrogels in bone-tissue engineering

Park¹

2011

Med Oral

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Many different types of scaffold materials have been used for tissue engineering applications, and hydrogels form one group of materials that have been used in a wide variety of applications. Hydrogels are hydrophilic polymer networks and they represent an important class of biomaterials in biotechnology and medicine because many hydrogels exhibit excellent biocompatibility with minimal inflammatory responses and tissue damage. Many studies have demonstrated the use of hydrogels in bone-tissue engineering applications. In this report, the summary was conducted on various kinds of polymers and different modification methods of hydrogels to enhance bone formation. The results revealed that hydrogels are applied for bone regeneration and that the modification of hydrogels with bioactive molecules or cell-based approaches resulted in significant increases in new bone formation. This suggests that the use of hydrogels with modification may offer an option for bone-tissue engineering, and further research is needed to identify the biological and physical properties of hydrogels.

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The effect of photopolymerization on stem cells embedded in hydrogels

Cited by 378 publications

References 54 publications

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Complementary, Semiautomated Methods for Creating Multidimensional PEG-Based Biomaterials

Poly(vinyl alcohol)-heparin biosynthetic microspheres produced by microfluidics and ultraviolet photopolymerisation

The use of hydrogels in bone-tissue engineering

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