The effect of plant density and macronutrient nutrition on Iris shoot cultures

Pryce, S.; Lumsden, P. J.; Berger, F.; Nicholas, J. R.; Leifert, Carlo

doi:10.1007/978-94-011-0790-7_6

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…The problem of low survival of micropropagated plants, when they were transferred into ex vitro conditions, was observed for many other plant species, such as Delphinium sp. (Pryce et al 1993) or Notocactus magnificus (Medeiros et al 2006). It is probably due to nonfunctional stomata, weak root system and poorly developed cuticules in the cultured plants (Estrada-Luna et al 2001;Isutsa 2004).…”

Section: Acclimatization Of Plantlets Ex Vitromentioning

confidence: 99%

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

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The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 lmol L -1 TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 lmol L -1 ) and 6-benzylaminopurine (BAP) (8 lmol L -1 ). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

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Section: Acclimatization Of Plantlets Ex Vitromentioning

confidence: 99%

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

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“…All data were viewed as repeated measurements on genetically uniform material and analyzed by the methods described in Pryce et al (1993).…”

Section: Discussionmentioning

confidence: 99%

“…This was thqught to be an important reason for the relatively low losses due to accidental bacterial contamination of such plant species. It was also discovered that adjusting the initial pH of media to 3.5 had no negative effect on the growth rate of Delphinium shoot cultures which do not reduce the medium pH and which suffer severe losses due to accidental bacterial contamination (Leifert et al 1992b;Pryce et al 1993). As a result of these investigations it appeared to be feasible to use artificial acidification of the growth medium as a method for prevention of accidental bacterial contamination.…”

Section: Introductionmentioning

confidence: 96%

Effect of medlium acidification on filamentous fungi, yeasts and bacterial contaminats inDelphinium tissue cultures

Leifert

Waites²,

Keetley³

et al. 1994

Plant Cell Tiss Organ Cult

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“…Ακηζεέηςξ, ζηζξ πνμενπυιεκεξ απυ θφθθα ηαθθζένβεζεξ παναηδνήεδηε ζπεηζηά ζδιακηζηά εεηζηή ζοζπέηζζδ ιυκμ ιεηαλφ ηςκ ζοβηεκηνχζεςκ K ηαζ Mg (r 2 =0.848). Υνδζζιμπμζχκηαξ δζαημζιδηζηά είδδ θοηχκ, υπςξ ημ Iris, μζ Pryce et al (1994) έδεζλακ υηζ μζ αολδιέκεξ ζοβηεκηνχζεζξ K ηαζ P ζπεηίγμκηακ εεηζηά ηαζ ζδιακηζηά ιε ηδκ in vitro ακάπηολδ ηςκ ηαθθζενβεζχκ. ε ζφβηνζζδ ιε ηα θοηά ζλμφ ηαζ ημοξ λεκζζηέξ ημοξ, μζ ηαθθζένβεζεξ ηάθςκ ακαιέκεηαζ κα πανμοζζάζμοκ ιεβαθφηενδ δναζηδνζυηδηα ηοηηανζηήξ δζαίνεζδξ ηαζ ακηζβναθήξ DNA.…”

Section: βηνδνθηκέο κεκβξαληθνύ δπλακηθνύ θπηηάξσλunclassified

Εφαρμογή βιοτεχνολογικών μεθόδων στο φυτό ιξός (viscum album L.) για την παραγωγή αντικαρκινικών ουσιών

Μπαρμπεράκη¹

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Αηζζάλνκαη ηελ αλάγθε θαη ηελ ππνρξέσζε λα επραξηζηήζσ ζεξκά όζνπο ζπλέβαιιαλ κε νπνηνδήπνηε ηξόπν ζηελ νινθιήξσζε ηεο παξνύζαο κειέηεο.Ιδηαηηέξσο, ζα ήζεια λα επραξηζηήζσ ηνλ Καζεγεηή θ. πύξν Κίληδην γηα ηελ ακέξηζηε ζπκπαξάζηαζε θαη θαζνδήγεζή ηνπ, γηα ηηο πνιύηηκεο ππνδείμεηο ηνπ θαη γηα ηελ ζπλερή βνήζεηά ηνπ.Αθόκε, ζα ήζεια λα επραξηζηήζσ ζεξκά ηνλ Αλαπιεξσηή Καζεγεηή θ.

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The effect of plant density and macronutrient nutrition on Iris shoot cultures

Cited by 6 publications

References 5 publications

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Effect of medlium acidification on filamentous fungi, yeasts and bacterial contaminats inDelphinium tissue cultures

Εφαρμογή βιοτεχνολογικών μεθόδων στο φυτό ιξός (viscum album L.) για την παραγωγή αντικαρκινικών ουσιών

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