The effect of plant growth regulators on the in vitro regeneration capacity in some horticultural crops and rare endangered plant species

Mitrofanova, I.V.; Лесникова-Седошенко, Н.П.; Chelombit, S.V.; Zhdanova, I.V.; Иванова, Н Н; Mitrofanova, O.V.

doi:10.17660/actahortic.2022.1339.24

Cited by 4 publications

(2 citation statements)

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“…Plant regeneration in vitro is a process in which explants undergo cellular division and differentiation and then form tissues and organs throughout the growth period [33][34]. The achievement of plant regeneration depends on numerous things, namely on explant types [35], nutrients [36], PGR types, their concentrations and combinations [37][38][39][40][41][42], the age of the mother plant from which the explant is separated [43], the season of explant separation, temperature, illumination [44], etc.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

Melyan,

Santrosyan

2024

FFHD

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Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material. Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars. Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit. Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested. Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet. Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

Melyan,

Santrosyan

2024

FFHD

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“…It is known that in vitro morphogenesis depends on many factors such as material genotype, type of explant, its orientation, light intensity, temperature, gas composition, and nutrient medium components, including growth regulators [14][15][16]. An important task is the selection of the nutrient media for growth supporting introduced into in vitro culture explants.…”

Section: Selection Of the Nutrient Medium For Peach Microshoot Develo...mentioning

confidence: 99%

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

Bilyak,

Sidyakin,

Bulavin

et al. 2023

E3S Web Conf.

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The article discusses the introduction in vitro, selection of the nutrient medium and plant growth regulator effect on the microshoot development of the perspective fruit crop Prunus persica ‘Dostoyniy’. Murashige and Skoog (MS), Gamborg and Eveleg (B5), Blaydes (BL), McCown (WPM) media supplemented with 0.5 mg/l 6-BAP, 0.1 mg/l IBA; 1.0 mg/l 6-BAP, 0.025 mg/l IBA; 0.48 mg/l KIN, 0.1 mg/l IBA; 0.96 mg/l KIN, 0.025 mg/l IBA and succinic acid were used in our research. According to obtained results, the most optimal season for the introduction of P. persica ‘Dostoiniy’ into in vitro culture was summer. The use of alcohol-, and chlorine-containing sterilizing agents in combination with antibiotics (or without them) provides aseptic material. BL and B5 media supplemented with 0.5 mg/l 6-BAP and 0.1 mg/l IBA, as well as 1.0 mg/l 6-BAP and 0.025 mg/l IBA promote microshoot development in vitro. Succinic acid in the culture medium at concentration of 5 and 10 g/l contributes to an additional increase in the multiplication rate.

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Anatomy, ploidy level, and essential oil composition of Hyssopus officinalis ʻNikitskiy Beliyʼ in vitro and ex situ

Bulavin,

Ivanova,

Miroshnichenko

et al. 2024

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Clonal micropropagation is a biotechnological method for plant multiplication. The existing data on the structure of organs in vitro, genetic stability, and essential oil composition are limited for Hyssopus officinalis L., so this study was aimead at investigating these aspects under a short period of in vitro culturing.Materials and methods. Plants of Hyssopus officinalis ʻNikitskiy Beliyʼ cultivated ex situ, in vitro and ex vitro were analyzed. Conventional methods were applied to study plant anatomy, ploidy level, and relative DNA content, as well as to extract and analyze essential oil. Statistical analysis was performed using the Past 4.03 software.Results. According to the results obtained, with 6-BAP introduced into MS nutrient medium in optimal concentrations (0.3– 0.5 mg/L), the general in vitro structure of leaf blades in the developed microshoots was similar to those in ex situ plants, while the qualitative and quantitative changes observed were induced by the effect of specific culturing conditions and plant rejuvenation. The analysis of the ploidy level and relative DNA content in the nuclei isolated from the leaf tissue cells of the microshoots ex vitro after adaptation revealed no changes compared to the ex situ leaf parameters. The mass fraction of essential oil and its component composition in the mother plants and ex vitro regenerants were similar.Conclusion. Cultivation of Hyssopus officinalis ʻNikitskiy Beliyʼ microshoots on MS nutrient medium with 6-BAP optimal concentrations promotes morphogenesis without significant deviations in the ploidy level, relative DNA content, essential oil yield, or its component composition. The developed protocol for clonal micropropagation of Hyssopus officinalis ʻNikitskiy Beliyʼ provides clones identical to the ex situ plants.

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The effect of plant growth regulators on the in vitro regeneration capacity in some horticultural crops and rare endangered plant species

Cited by 4 publications

References 15 publications

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

Anatomy, ploidy level, and essential oil composition of <i>Hyssopus officinalis</i> ʻNikitskiy Beliyʼ <i>in vitro</i> and <i>ex situ</i>

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