The effect of plant protein-based diet supplemented with dipeptide or free amino acids on digestive tract morphology and PepT1 and PepT2 expressions in common carp (Cyprinus carpio L.)

Ostaszewska, Teresa; Dąbrowski, Konrad; Kamaszewski, Maciej; Grochowski, Piotr; Verri, Tiziano; Rzepkowska, Małgorzata; Wolnicki, Jacek

doi:10.1016/j.cbpa.2010.06.162

Cited by 93 publications

(66 citation statements)

References 51 publications

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“…Several studies suggested that regulated PepT1 expression by dietary‐free amino acid, peptides and intact protein in fish could affect growth performance directly and/or indirectly (Ostaszewska et al . , ,b; Bakke et al . ; Liu et al .…”

Section: Introductionmentioning

confidence: 99%

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Wei

Liang

et al. 2015

Aquacult Nutr

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The intention of the study was to investigate the effect of ultrafiltered fish protein hydrolysate (UF) level on growth, feed utilization, apparent digestibility coefficients and proximal intestine peptide transporter 1 (PepT1) mRNA level for juvenile turbot (Scophthalmus maximus L.). Experimental diets (UF‐0, UF‐5, UF‐10, UF‐15 and UF‐20) were prepared containing about 68% plant protein, and fish meal protein was, respectively, replaced by 0%, 5%, 10%, 15% and 20% UF of dietary protein. Diet PP contained about 78% plant protein, and diet CAA contained about 10% crystalline amino acid mixture. All diets were fed to seven triplicate groups of turbot (initial weight 16.05 ± 0.03 g) for 68 days. Fish fed diet UF‐10 had an increasing tendency in growth compared with diets contained UF, while dietary UF level was not significantly correlated with specific growth rate and feed intake. Feed efficiency, protein efficiency ratio and protein productive value significantly correlated with dietary UF level, and fish fed diets contained low‐level UF had higher digestibility than that diets UF‐0, PP and CAA. There was a decreasing tendency in PepT1 expression level with dietary UF level. The results indicated that low‐level UF showed a positive effect on growth and feed utilization in juvenile turbot.

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Section: Introductionmentioning

confidence: 99%

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Wei

Liang

et al. 2015

Aquacult Nutr

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“…Enzymatic secretion during ontogenesis was studied on various Acipenseridae (Żółtowska et al, 1999;NaporaRutkowski et al, 2009), but the physiological and morphological impact of different diets was not completely determined. However, free amino acids (FAA) and free fatty acids (FFA) are known to stimulate digestive processes and the assimilation of nutrients, and they also may have influence on digestive tract morphology, feeding behavior or food intake (Ostaszewska et al, 2008;Napora-Rutkowski et al, 2009;Naz and Türkmen, 2009;Ostaszewska et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In order to achieve higher survival, growth and stress-resistance numerous nutritional studies are conducted every year (Jalali et al, 2008;Ostaszewska et al, 2010;Ostaszewska et al, 2011;Noori et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Kamaszewski¹,

Ostaszewska²,

Prusińska³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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The aim of this study was to compare the physiology and morphology of the digestive tract of Russian sturgeon (Acipenser gueldenstaedtii) larvae fed Artemia sp. nauplii enriched or non-enriched with essential fatty acids (EFA). Physiology was evaluated by digestive enzyme activity analysis, while morphology was assessed with histological methods. The larvae were divided into two groups, in which fish were fed either pure Artemia sp. nauplii or Artemia enriched in EFA. Both groups had similar survival rates, but fish fed EFA-enriched Artemia displayed higher, body weight and length. At the end of the experiment (22 dph): 1) the activities of lipase and leucine aminopeptidase were similar in both groups; 2) the activities of trypsin, alkaline phosphatase and γ-glutamyltransferase were insignificantly higher in fish fed pure Artemia; 3) the activity of α-amylase was significantly higher in the EFA-enriched feeding group; 4) lower hepatocyte lipid vacuole diameters and hepatocyte proliferation were measured in the EFA-enriched group. Lipid accumulation was observed in the anterior intestine of fish fed pure Artemia on the 15 th and 22 nd dph. The epithelial turnover was significantly lower in the EFAenriched group, in the posterior intestine on the 15 th dph, but no differences occurred between the groups on the 22 nd dph, in either anterior or posterior intestine. In conclusion, the study revealed a positive effect of the EFA-enriched Artemia-based diet on the physiology and morphology of the digestive system of Russian sturgeon larvae.

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“…The affinity of PepT1 and its stereoselective capacity differ for different types of peptides, suggesting that its activity and expression may be modulated through the diet, particularly when different protein sources are utilized (Ostaszewska et al 2010a; Ostaszewska et al 2010b). However, to our knowledge, there is no information regarding the response at the mRNA level of such transporter when vegetable sources are used as a substitute for fish meal in the diet of marine fish.…”

Section: Introductionmentioning

confidence: 99%

PepT1 mRNA expression levels in sea bream (Sparus aurata) fed different plant protein sources

et al. 2013

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The expression and regulation of intestinal oligopeptide transporter (PepT)-1 when vegetable sources are used as a substitute for fish meal in the diet of marine fish has not yet been explored. In the present study, as part of our ongoing work on elucidating PepT1 gene expression in relation to different dietary treatments, we have now isolated and deposited in Genbank database (accession no. GU733710) a cDNA sequence representing the PepT1 in the sea bream (Sparus aurata). The “de novo” prediction of the three-dimensional structure of PepT1 protein is presented.We also analyzed diet-induced changes in the expression of PepT1 mRNA via real-time RT-PCR using the standard curve method. Sea bream were fed for 140 days with one of the following four diet formulations (43% protein/21% lipid): a control fast growth-promoting diet (C), and three diets with the same formulation but in which 15% of the fish meal was substituted by protein concentrates either from lupine (LPC), chick pea (CPC), or green pea (PPC). Fish fed PPC had significantly (p < 0.05) lower levels of PepT1 transcripts in the proximal intestine than the controls, whereas PepT1 transcript levels in fish fed LPC or CPC were not significantly different from the controls. Although growth was similar between fish fed with different diets during the first 72 days of feeding, growth of the fish fed with PPC was reduced during the second part of the trial and was significantly (p < 0.05) lower than fish fed LPC and CPC diets by the end of the experiment. Correlation between these results and fish growth performances highlights that the intestinal PepT1 mRNA level may serve as a useful marker of the dietary protein quality and absorption efficiency.

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The effect of plant protein-based diet supplemented with dipeptide or free amino acids on digestive tract morphology and PepT1 and PepT2 expressions in common carp (Cyprinus carpio L.)

Cited by 93 publications

References 51 publications

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

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PepT1 mRNA expression levels in sea bream (Sparus aurata) fed different plant protein sources

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