The effect of polyacrylic acid treatment on the susceptibility of Nicotiana tabacum cv. xanthi-nc to tobacco mosaic virus

Cassells, A. C.; Barnett, A.; Barlass, M.

doi:10.1016/0048-4059(78)90071-1

Cited by 23 publications

(9 citation statements)

References 12 publications

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“…Treating the challenged or lower leaves with polyanions such as polyacrylic acid before challenge inoculation also reduces the number of lesions formed (Gianinazzi & Kassanis, I974;Kassanis & White, 1974Cassells et al 1978 ). Our results showing acquired systemic susceptibility in N. glutinosa are thus the direct opposite of the acquired systemic resistance found in hypersensitive varieties of N. tabacum.…”

Section: Discussionsupporting

confidence: 48%

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Acquired Systemic Susceptibility to Infection by Tobacco Mosaic Virus in Nicotiana glutinosa L.

Fraser¹,

Loughlin²,

Whenham³

1979

Journal of General Virology

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SUMMARYIn Nicotiana glutinosa L. formation of local lesions on lower leaves inoculated with tobacco mosaic virus increased the susceptibility of the upper leaves to infection in a subsequent inoculation. The increase in susceptibility was detected as an increase of up to 3"5-fold in the number of lesions produced on the upper leaf and a corresponding increase in the amount of virus RNA synthesized. The concentration of endogenous abscisic acid in the upper leaves was negatively correlated with susceptibility to infection. This acquired systemic susceptibility to infection in N. glutinosa is in direct contrast to the acquired systemic resistance to infection reported to occur in hypersensitive varieties of Nicotiana tabacum under similar conditions. Mechanisms which might be involved in the acquisition of systemic resistance or susceptibility are discussed.

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Section: Discussionsupporting

confidence: 48%

“…They suggest that the loss of turgidity may reduce the susceptiNlity of surface sites to infection by TMV. Experimental support for this hypothesis comes from their observation that treatment of leaves with an anti-transpirant abolishes the polyacrylic acid-induced resistance (Cassells et al 1978).…”

Section: Discussionmentioning

confidence: 88%

Acquired Systemic Susceptibility to Infection by Tobacco Mosaic Virus in Nicotiana glutinosa L.

Fraser¹,

Loughlin²,

Whenham³

1979

Journal of General Virology

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“…The reduction in number of lesions formed when healthy plants were sprayed with ABA before inoculation is also consistent with this correlation. Whether ABA is the direct cause of altered lesion formation, or indirectly involved through an effect on leaf water status and mechanical susceptibility to inoculation (Cassells et aL, 1978) remains to be seen.…”

Section: Discussionmentioning

confidence: 99%

Are 'Pathogenesis-related' Proteins Involved in Acquired Systemic Resistance of Tobacco Plants to Tobacco Mosaic Virus?

Fraser¹

1982

Journal of General Virology

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SUMMARYFour host-coded 'pathogenesis-related' proteins accumulate systemically in locallesion-forming varieties of tobacco after infection with tobacco mosaic virus. It has been suggested that they are involved in the acquired systemic resistance of plants to a second inoculation. Pathogenesis-related protein concentration and amount of resistance (reduction in size and number of lesions formed in the second inoculation) were measured at various times after the first inoculation. The results showed no quantitative or temporal relationship between amounts of resistance and pathogenesis-related proteins. In particular, resistance could be demonstrated in leaves before detectable accumulation of pathogenesis-related protein. Abscisic acid sprayed on plants induced an apparent resistance without inducing pathogenesisrelated proteins. Low doses of methyl benzimidazol-2yl-carbamate caused accumulation of pathogenesis-related protein but not resistance. Nicotiana glutinosa plants accumulated large amounts of a similar protein after infection, but became more susceptible to a second inoculation. All these results suggest that the pathogenesis-related proteins do not play a central role in the mechanism of acquired systemic resistance.

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“…Solutions of polyacrylic acid (P A, mol wt 3500, Allied Colloids Co., Bradford) were neutralized with NaOH to pH 6.5 before application, and were sprayed onto cowpea leaves or cucumber cotyledons in solution with 0.05% Tween 20 as a wetting agent (CASSELLS and FLYNN 1978). Concentrations of 20 and 40 mg ml-1 were tested.…”

Section: Polyacrylic Acidmentioning

confidence: 99%

Induced Resistance to Viral Infection and Soluble Protein Alterations in Cucumber and Cowpea Plants

Coutts

Wagih

1983

Journal of Phytopathology

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The formation of tobacco necrosis virus‐elicited local lesions led to a localized “resistance” in cowpea leaves and cucumber cotyledons with an “acquired systemic resistance” induced in cucumber first leaves, following homogolous challenge. The “resistance” was against symptom expression rather than against virus multiplication. A similar “resistance” in both species could be elicited by spraying healthy tissue with polyacrylic acid 4 days before inoculation or osmotically‐stressing cowpea leaves 24 h before inoculation. Virus‐elicited necrosis resulted in reproducible alterations to the soluble protein profile of both species. Apparently novel host proteins, termed the α and β fractions in cowpea leaves and the γ fraction in cucumber tissue were induced during necrosis, and quantitatively reduced by concomitant actinomycin D treatment. Tissue of both species showing “resistance” contained either small amounts or more often none of the apparently novel fractions and any direct involvement in “resistance” is thought unlikely. It is proposed that the tissue exhibiting a degree of “resistance” to virus inoculation has an altered susceptibility to infection. This altered susceptibility appears to be engendered by treatments (e.g. PA spraying, osmotic stressing or previous inoculations on the same plants) that reduce the turgidity of the cells to be challenged.

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The effect of polyacrylic acid treatment on the susceptibility of Nicotiana tabacum cv. xanthi-nc to tobacco mosaic virus

Cited by 23 publications

References 12 publications

Acquired Systemic Susceptibility to Infection by Tobacco Mosaic Virus in Nicotiana glutinosa L.

Acquired Systemic Susceptibility to Infection by Tobacco Mosaic Virus in Nicotiana glutinosa L.

Are 'Pathogenesis-related' Proteins Involved in Acquired Systemic Resistance of Tobacco Plants to Tobacco Mosaic Virus?

Induced Resistance to Viral Infection and Soluble Protein Alterations in Cucumber and Cowpea Plants

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