The effect of polydisperse fucoidans from Fucus vesiculosus on Hep G2 and Chang liver cells

Zhurishkina, Elena V.; Stepanov, S. I.; Ayrapetyan, Olga N.; Skorik, Yury A.; Vlasova, E. N.; Kruchina-Bogdanov, I. V.; Лебедев, Д. В.; Kulminskaya, Anna A.; Lapina, I. M.

doi:10.1016/j.bcdf.2019.100209

Cited by 9 publications

(4 citation statements)

References 49 publications

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“…The sulfate content of the purified fucoidan significantly decreased (from 27.6 ± 0.2% in F to 18.3 ± 0.3% in Fo), while according to IR spectroscopy data the position of sulfate groups at C2 and C4 of the fucose residue (predominantly equatorial) did not change. Since fucoidan is a polydisperse mixture of sulfated polysaccharide molecules that differ in size and chemical composition [ 38 ], it seems that the change in the ratio of monosaccharide content in the purified sample is caused, among other things, by the loss of some polysaccharide fractions because of dialysis.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea

Ayrapetyan

Obluchinskaya

Zhurishkina

et al. 2021

Biology

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Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications in medicine and the food industry. In this study, we compare the antibacterial properties of two fractions of fucoidan from the brown algae Fucus vesiculosus gathered in the littoral of the Barents Sea and sampled at different stages of purification. The crude fraction of fucoidan was isolated from algae by extraction with aqueous ethanol and sonication. The purified fraction was obtained by additional treatment of the crude fraction with a solution of calcium chloride. The structural features of both fractions were characterized in detail and their antibacterial effects against several Gram-positive and Gram-negative bacteria were compared by photometry, acridine orange staining assay, and atomic force microscopy. Fucoidan inhibited growth in all of the above microorganisms, showing a bacteriostatic effect with minimum inhibitory concentrations (MIC) in the range between 4 and 6 mg/mL, with E. coli being the most sensitive to both fractions. Changes in the chemical composition after treatment of the crude fraction with a solution of calcium chloride led to a decrease in the content of sulfates and uronic acids and diminished antibacterial activity.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea

Ayrapetyan

Obluchinskaya

Zhurishkina

et al. 2021

Biology

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“…We found that the expression of p62 decreased with the increase of fucoidan concentration, while the expression of Beclin-1 protein was increased. Therefore, through the (Park et al, 2011), breast cancers (by downregulating the PI3K/AKT/m-TOR pathway) (Zhang et al, 2016), liver cancers (by increasing the formation of autophagosomes) (Zhurishkina et al, 2020), and cardiac diseases (by upregulating the JAK2/STAT3 pathway) (Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, through the detection of autophagy marker proteins, the results indicated that fucoidan could induce autophagy in the two kinds of breast cancer cells, and this induced effect of fucoidan was more obvious in MDA‐MB‐231 cells. Additionally, recent studies have found that fucoidan, as an autophagy regulator, plays an important role in other diseases, such as gastric cancers (by the upregulation Beclin‐1 and the conversion of LC3‐I to LC3‐II) (Park et al, 2011), breast cancers (by downregulating the PI3K/AKT/m‐TOR pathway) (Zhang et al, 2016), liver cancers (by increasing the formation of autophagosomes) (Zhurishkina et al, 2020), and cardiac diseases (by upregulating the JAK2/STAT3 pathway) (Zhang et al, 2020). These studies have shown that the autophagic effect of fucoidan‐induced was very obvious and had a good therapeutic effect on diseases.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of fucoidan on breast cancer cells and potential enhancement of their sensitivity to chemotherapy by regulating autophagy

Zhang

Xue

Wang

et al. 2021

Phytotherapy Research

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Fucoidan is a marine‐origin sulfated polysaccharide that has gained attention for its anticancer activities. However, the inhibitory effect of fucoidan on breast cancers by regulating autophagy and its mechanism are not clear, and the chemotherapeutic sensitization of fucoidan is largely unknown. In the present study, the anticancer potential of fucoidan was revealed in MCF‐7 and MDA‐MB‐231 cells. Additionally, we also studied the chemotherapeutic sensitization of fucoidan by combining chemotherapeutic drugs doxorubicin (ADM) and cisplatin (DDP) with fucoidan on breast cancer cells. In the two kinds of human breast cancer cells, cell viability was determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay. Apoptosis was examined with flow cytometry. Transfection assay was used to examine autophagy flow. Western blot was used to examine the expressions of related proteins. Results suggested that fucoidan could induce autophagy and might enhance the sensitivity of breast cancer cells to chemotherapeutic drugs. Mechanistically, fucoidan induced autophagy in breast cancer cells by down‐regulating m‐TOR/p70S6K/TFEB pathway. In conclusion, our research revealed that fucoidan could induce autophagy of breast cancer cells by mediating m‐TOR/p70S6K/TFEB pathway, thus inhibiting tumor development. Furthermore, fucoidan might enhance the sensitivity of breast cancer cells to ADM and DDP, and this enhancement was related to autophagy.

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“…Additionally, Fu exhibits a special affinity for various cells and receptors, including macrophages, chemokines, growth factors, and P-selectin. This affinity enables the enhanced delivery of drugs to specific target locations, further amplifying its potential in drug delivery applications [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Fucoidan and dendrimer-based nanocapsule exhibiting effectiveness in methotrexate controlled delivery towards rheumatoid arthritis treatment

Nguyen,

Doan,

Tran

et al. 2023

Adv. Nat. Sci: Nanosci. Nanotechnol.

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In recent years, nanomaterials have been intensively studied and applied in various fields, including pharmaceutical applications. This platform can act as a carrier for anticancer drugs or for insoluble bioactive compounds. To increase the stability and prolong the effect of anticancer drugs, we have incorporated a sulfated polysaccharide fucoidan (Fu) into PAMAM dendrimer G3.0 to form a G3.0-Fu complex. Then, a nano-sized encapsulated anticancer drug, methotrexate (MTX), was successfully embedded in the synthesised dendrimer complex namely G3.0-Fu/MTX. Newly synthesised G3.0-Fu/MTX was characterised by transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential measurement. Additionally, the loading efficiency of MTX was assessed via UV spectroscopy. Our findings revealed that upon combining with Fu, the G3.0 nanoparticle size increased from 4.3 ± 1.1 nm to 56 ± 6 nm. The changes in zeta potential aligned with drug entrapment efficiency and the results from TEM and DLS. The drug release activity of G3.0-Fu/MTX was increased compared to free MTX after 24 h. G3.0-Fu also showed high cytocompatibility in fibroblast cells. Taken together, the G3.0-Fu could be used to increase the encapsulation of several kinds of hydrophobic drugs and G3.0-Fu/MTX could be further studied in rheumatoid arthritis treatment.

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The effect of polydisperse fucoidans from Fucus vesiculosus on Hep G2 and Chang liver cells

Cited by 9 publications

References 49 publications

Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea

Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea

Inhibition of fucoidan on breast cancer cells and potential enhancement of their sensitivity to chemotherapy by regulating autophagy

Fucoidan and dendrimer-based nanocapsule exhibiting effectiveness in methotrexate controlled delivery towards rheumatoid arthritis treatment

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