The effect of priming and duration of oestradiol benzoate treatment before progesterone administration on embryo development and survival in anestrous recipient mares

Oquendo, Pedro S; Silva, Elisa S.M.; Oquendo, Fabiana MG; Cuervo-Arango, J.; Beletti, Marcelo Emílio

doi:10.1111/rda.14220

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…Furthermore, 24 h after the transfer of six embryos into an apparently optimal endometrial environment of a properly synchronized recipient mare, one embryo was already destroyed. This confirms the observation of a previous study in which a timing of 48 h after transfer was likely to be too long to recover embryos which did not resist a suboptimal environment [ 31 ].…”

Section: Discussionsupporting

confidence: 90%

Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares

Martínez-Boví,

Gaber,

Sala-Ayala

et al. 2024

Animals

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The effect of embryo reinsertion immediately after embryo flushing was studied. In Experiment 1, eight mares were used during 32 cycles (8 cycles in each group). For the first two groups, inseminated mares were flushed 8 days after ovulation and prostaglandin F2α was not administered: in group EF-ET (embryo flushing and embryo transfer) the embryo was reinserted in the same donor mare, while in the EF group, no further procedure was performed. In the third group (ET), non-inseminated mares (recipients) received a Day 8 embryo. Progesterone concentration was measured before EF/ET and 72 h after in the three groups. In Experiment 2, twelve mares were used during 17 cycles in two groups, EF-ET (n = 11) and ET (n = 6), as in Experiment 1, except that every mare was flushed 24 h after embryo transfer to retrieve the embryo. Fewer pregnancies resulted after transfer in EF-ET cycles (0/8, 0%) than in the ET group (6/8, 75%). Progesterone concentration decreased significantly (p = 0.05) 72 h after EF-ET but not in EF or ET cycles (p > 0.1). Three mares from the EF-ET showed full luteolysis and signs of endometritis. In Experiment 2, more (5/6; p = 0.08) grade 1 embryos were recovered in the ET compared to the EF-ET group (3/7); 4 embryos were graded 3–4 (were broken or had signs of degeneration) in the EF-ET group but none in the ET group. In both groups, capsule fragments were obtained as indicative of the presence of a recently destroyed embryo in the EF-ET (n = 3) and ET (n = 1) groups. Positive bacterial cultures were obtained in 2/11 and 1/6 embryo flushes from the EF-ET and ET groups, respectively.

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Section: Discussionsupporting

confidence: 90%

Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares

Martínez-Boví,

Gaber,

Sala-Ayala

et al. 2024

Animals

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show abstract

“…In fact, only capsule fragments (sign of a destroyed embryo) were found in 3 of 11 reinserted embryos, and even in one of 6 embryos transferred into an apparently optimal endometrial environment of a properly synchronized recipient mare, by only 24 h of transfer. This confirms the observation of a previous study in which a timing of 48 h after transfer was likely to be too long to recover embryos which did not resist a suboptimal environment [32].…”

Section: Discussionsupporting

confidence: 90%

“…Furthermore, it is unknown too, whether these three embryos were able to develop normally because the endometrial environment was optimal or because these embryos were more resistant to a hostile environment. In this regard, it has been shown that even though most embryos require an endometrial environment that has been primed with oestrogens, around 40 percent of them can survive in recipient mares which had been treated with exogenous progesterone only [32,33], which are assumed to have a suboptimal endometrial environment [34].…”

Section: Discussionmentioning

confidence: 99%

Pregnancy and Luteal Responses to Embryo Reinsertion Following Embryo Flushing in Donor Mares

Martínez-Boví,

Gaber,

Sala-Ayala

et al. 2024

Preprint

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The effect of embryo reinsertion immediately after embryo flushing was studied to determine its effect on luteal function, pregnancy outcome, endometrial bacterial contamination, and embryo morphology. In Experiment 1, eight mares were used during 32 cycles (8 cycles in each group): for the first two groups, inseminated mares were flushed 8 days after ovulation and prostaglandin F2α was not administered: in group EF-ET (embryo flushing and embryo transfer) the embryo was reinserted in the same donor mare, while in the EF group, no further procedure was performed. In the third group (ET), non-inseminated mares (recipients) received a Day 8 embryo 5 to 8 days after ovulation. Progesterone concentration was measured before EF/ET and 72 h after in the 3 groups. Pregnancy diagnosis was performed within 6 days of transfer. In Experiment 2, twelve mares were used during 17 cycles in two groups: EF-ET (n=11) and ET (n=6), as in Experiment 1, except that every mare was flushed 24 h after embryo transfer to retrieve the embryo. Fewer pregnancies resulted after transfer in EF-ET cycles (0(8, 0%) than in the ET group (6/8, 75%). Progesterone concentration decreased significantly (p=0.05) 72 h after EF-ET, but not in EF or ET cycles (p>0.1). Three mares from the EF-ET showed full luteolysis and signs of endometritis. In Experiment 2, more (5/6; p=0.08) grade I embryos were recovered in the ET compared to the EF-ET group (3/7); 4 embryos were graded III-IV (were broken or had signs of degeneration) in the EF-ET group, but none in the ET group. In both groups, capsule fragments were obtained as indicative of the presence of a recently destroyed embryo in the EF-ET (n=3) and ET (n=1) groups. Positive bacterial cultures were obtained in 2/11 and 1/6 embryo flushes from the EF-ET and ET groups, respectively.

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Effect of duration of estradiol exposure on embryo survival and endometrial gene expression in anestrous embryo recipient mares

Sant'Anna Monteiro da Silva,

Sanches Oquendo Júnior,

Gaspari Oquendo

et al. 2024

Theriogenology

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The effect of priming and duration of oestradiol benzoate treatment before progesterone administration on embryo development and survival in anestrous recipient mares

Cited by 6 publications

References 7 publications

Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares

Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares

Pregnancy and Luteal Responses to Embryo Reinsertion Following Embryo Flushing in Donor Mares

Effect of duration of estradiol exposure on embryo survival and endometrial gene expression in anestrous embryo recipient mares

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