Embryo transfer (ET) is a common practice in equine-assisted reproduction programs. Mares are seasonal polyoestrous long day breeders and, the majority of them, undergo a period of winter anestrus (Aurich, 2011). Therefore, the use of hormonal protocols in seasonal anestrous mares is a crucial tool to increase the efficiency of ET clinical programs since it allows the possibility of transferring embryos outside the breeding season.Although progesterone alone can be used to prepare anestrous recipient mares to receive embryos and maintain the pregnancy to term (Hinrichs et al., 1987), pre-exposure to oestradiol before progesterone dominance appears to improve the endometrial environment to increase embryo survival (Cuervo-Arango, Claes, de

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