The effect of Propionibacterium acnes on maturation of dendritic cells derived from acne patients' peripherial blood mononuclear cells.

Abstract

Propionibacterium acnes (P. acnes) has been implicated in the pathogenesis of acne vulgaris which is the most common cutaneous disorder. It has a proinflammatory activity and takes part in immune reactions modulating the Th1/Th2 cellular response. The exposure of dendritic cells (DCs) to whole bacteria, their components, cytokines or other inflammatory stimuli and infectious agents induces differentiation from immature DCs into antigen-presenting mature DCs. The aim of the study was to evaluate the capability of P. acnes to induce the maturation of DCs. We stimulated monocyte derived dendritic cells (Mo-DCs) from acne patients with various concetrations of heat-killed P. acnes (10 6 -10 8 bacteria/ml) cultured from acne lesions. The results showed an increase in CD80+/CD86+/DR+ and CD83+/CD1a+/DR+ cells percentage depending on the concetration of P. acnes. The expression of CD83 and CD80 (shown as the mean fluorescence intensity -MFI) increased with higher concetrations of P. acnes. There were also significant correlations between MFI of CD83, CD80, CD86 and concetration of P. acnes. The study showed that P. acnes in the concetration of 10 8 bacteria/ml is most effective in the induction of Mo-DCs maturation. Futher studies concerning the influence on the function of T cells are needed. Short communicationto whole bacteria, their components, cytokines or other inflammatory stimuli and infectious agents induces differentiation of immature DCs into antigen-presenting mature DCs. DCs maturation is a process where major histocompatibility complex (MHC) class I or II and costimulatory molecules (e.g. CD80 and CD86) are upregulated and at the same time specific markers like CD83 and p55 are expressed [5].The aim of the study was to evaluate the capability of P. acnes to induce the maturation of autologous DCs. Patients and methods

Sebocytes contribute to skin inflammation by promoting the differentiation of T helper 17 cells

“…When this commensal bacterium proliferates, it can come into contact with DCs and activate their maturation with the consequent immune response crucially depending on presence of local commensals or pathogens, biofilm production and additional signals from tissue cells 38 . It has been reported that DCs stimulated with P.acnes show an increased expression of adhesive molecules and cytokines, which is similar to DCs activated by LPS 39,40 . In presence of naïve T cells, P.acnes-matured DCs induced a strong secretion of IFN-γ that is comparable to LPS-matured DCs confirming the capacity of P.acnes in eliciting…”

Sebocytes contribute to skin inflammation by promoting the differentiation of T helper 17 cells

“…It has been reported that DCs stimulated with P. acnes show an increased expression of adhesive molecules and cytokines, which is similar to DCs activated by LPS . In the presence of naive T cells, P. acnes ‐matured DCs induced a strong secretion of IFN‐γ that is comparable with LPS‐matured DCs, confirming the capacity of P. acnes in eliciting a powerful Th1‐type immune response .…”

Epigallocatechin-3-Gallate Improves Acne in Humans by Modulating Intracellular Molecular Targets and Inhibiting P. acnes

“…acnes, a Gram-positive anaerobic bacterium, is a commensal of human skin, and its overgrowth is closely implicated in the progression of inflammation in acne (Kim, 2005;Michalak-Stoma et al, 2008). Until recently, various antibiotics have been used to control the overgrowth of P. acnes, yet increasing antibiotic resistance (Strauss et al, 2007;Thiboutot, 2008) and biofilm formation (Burkhart and Burkhart, 2007) lead to a poor outcome.…”