The effect of protected methionine and lysine on milk production and composition on grass-silage based diets

Younge, Bridget; Murphy, John J.; Rath, M.; Sloan, B. K.

doi:10.1017/s0308229600029330

Cited by 7 publications

(13 citation statements)

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“…On the other hand, the diets supplied similar amounts of PDI (PDIE), although slightly below the requirement. Dry matter (and energy) intake was unaffected by protected amino acid supplementation, which agrees with earlier results in cows fed grass-silage-based diets (Robert et al, 1994;Pisulewski and Kowalski, 1999a,b;Younge et al, 2001;Pisulewski et al, 2002). Similarly, postruminal (abomasal) infusions of lysine or methionine did not affect the above measurements .…”

Section: Discussionsupporting

confidence: 90%

“…In experiments using cows fed grass silage, milk protein yield responses to postruminal supply of methionine (or methionine with lysine) were inconsistent, ranging from apparent increases (Xu et al, 1998;Younge et al, 2001) to no effects (Pisulewski and Kowalski, 1999a,b;Vanhatalo et al, 1999;Varvikko et al, 1999;Pisulewski et al, 2002). The lack of response to ruminally-protected methionine is unexpected, since theoretically grass silage-based diets are considered to be deficient in methionine absorbed in the intestine .…”

Section: Introductionmentioning

confidence: 99%

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Effects of protected methionine and variable energy supply on lactational responses in dairy cows fed grass silage-based diets

Kowalski

Pisulewski²,

Görgülü³

2003

J. Anim. Feed Sci.

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Twelve multiparous Holstein cows (average body weight 610 kg; 56 -84 d after calving at the start of the trial) in their second, third or fourth lactation were assigned to two-factorial (2 x 2) arrangement of treatments, in a balanced changeover design, involving two levels of energy (adequate, AE, 100% vs low, LE, 80% of INRA requirements) and two levels of ruminally-protected DL-methionine (Smartamine TM M: 0 vs 20 g/d). The treatments were M0-AE, M20-AE, M0-LE and M20-LE. The calculated intestinal concentrations of lysine and methionine (% PDI) were: 6.9 and 1.7, 6.9 and 2.1, 6.9 and 1.7, 6.9 and 2.2, respectively. The AE and LE diets contained (% DM): grass silage 44 and 50, and concentrates 56 and 50, respectively. Low energy intake was obtained by reducing the total amount of feed offered (from 19.6 kg DM in the AE diets to 16.2 kg in the LE diets). The diets provided 100% of requirements for protein digested in the small intestine (PDI). Average milk yield tended to be increased in the cows fed AE vs LE diets (averaging 27.4 vs 26.5 kg), but the differences were not significant. Milk fat, lactose and SNF contents did not respond to the treatments. Feeding ruminally protected methionine slightly, but significantly, increased milk protein content (2.91 vs 3.07%, for M0 vs M20 diets; P<0.01), with no effect on milk protein yield. Milk produced by the AE-fed cows contained significantly more casein-N (P<0.05) and less NPN (P<0.01) compared with the LE cows. Methionine supplementation resulted in significant increases in the contents in milk of total-N, protein-N, casein-N and whey-N (%), with no effect on NPN and urea-N (% in milk) and protein-N % of total N, casein-N % of total-N. The effect of methionine on the content of nitrogen fractions in milk was more apparent in the cows fed the LE diets. PROTECTED METHIONINE AND ENERGY IN DIETS FOR COWSIt is concluded that supplementing dairy cows fed grass silage-based diets with ruminally-protected methionine had no effect on milk yield but resulted in apparent changes in milk composition. The reaction of cows to additional supply of absorbable methionine was particularly evident in energyunderfed cows.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effects of protected methionine and variable energy supply on lactational responses in dairy cows fed grass silage-based diets

Kowalski

Pisulewski²,

Görgülü³

2003

J. Anim. Feed Sci.

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“…Amino acid supplementation had no significant effect on mean dry matter intake or mean milk yield (Table 2). These effects confirm earlier findings of several studies using grass silage-based diets (Robert et al, 1994;Chillard et al, 1995: Younge et al, 1995. On the other hand, feeding protected amino acids may increase dry matter intake of grass silage diets in early lactation (Xu et al, 1998).…”

Section: Discussionsupporting

confidence: 89%

“…Although the ration provided an excess of N, as indicated by high content of PDIN compared with that of PDIE (Table 2), milk urea was uniform across treatments and similar to values for cows fed either maize silage (Pisulewski et al, 1996) or grass silage (Xu et al, 1998) rations. Inconsistent responses of milk fat content and yield were in line with comparable studies (Robert et al, 1994;Chillard et al, 1995;Younge et al, 1995;Xu et al, 1998), in which, feeding protected lysine and methionine did not significantly alter these parameters. (Table 5) can be discussed in terms of energy and protein, including amino acids status of the dairy cows.…”

Section: Discussionsupporting

confidence: 85%

The effect of protected lysine and methionine on milk yield and its composition in lactating dairy cows fed grass silage-based rations

Pisulewski¹,

Kowalski²

1999

J. Anim. Feed Sci.

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Five multiparous Polish Red-and-White cows, weeks 8-12 of lactation, in a 5 x 5 Latin square treatment were fed a total mixed ration with grass silage and concentrates (58 and 42% DM). The five treatments were dietary supplements of protected L-lysine and DL-methionine (Smartamine™ ML) and DL-methionine (Smartamine™M) fed in the following amounts: I: 0 g/d + 0 g/d; II: 35 g/d + 0 g/d; III: 35 g/d + 10 g/d; IV: 35 g/d + 20 g/d; and V: 35 g/d + 30 g/d. The corresponding intestinal concentrations of lysine and methionine (% PDI) were: I: 6.89 and 1.88; II: 7.37 and 2.05; III: 7.32 and 2.29; IV: 7.31 and 2.54; V: 7.28 and 2.78, respectively. The DMI and milk yield were similar among treatments. In contrast, milk protein content was significantly increased (P<0.05) over the II treatment and then varied little. Milk fat content and yield varied inconsistently. Plasma metabolites such as glucose and (3-hydroxybutyrate were not affected and fell within physiological limits.Plasma free amino acids responded to the treatments, particularly lysine (PO.05) and methionine (P<0.001), producing a pattern of responses similar to that described for milk protein. Significant increases in milk protein content in the second treatment (Smartamine™ ML, 35 g/d) as resulting from improved postruminal supply of lysine (equal to needs), confirm the validity of the assumed requirement for this amino acid (i.e. 7.3% PDI). These increases could have also been due to a higher supply of limiting methionine. However, further improvements in intestinal supply of methionine, gradually meeting the assumed requirement (i.e. 2.5% PDI), had no effect on milk protein content. This lack of responses to methionine (treatments III-V), could have resulted either from a higher postruminal supply of this amino acid than that predicted or from a lower methionine requirement than that assumed.

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“…Out of the twenty naturally occurring amino acids, L-Lysine (Lys) is one of the 9 essential and commercially important amino acids, ecumenically found in naturally occurring proteins of all living organisms. Lys is often used as special chemical in medicament, chemical agent, food material, and feed additive (1)(2)(3). Recently, its production and purification technology have attracted great attention.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Study of Extraction of L-Lysine withSec-Octylphenoxy Acetic Acid in Sulfonated Kerosene

Zhang

Zhou

Wang

et al. 2014

Separation Science and Technology

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Extraction of L-lysine with sec-octylphenoxy acetic acid (CA-12) in sulfonated kerosene was investigated under different pH and CA-12 concentrations, and optimized conditions were determined. Half-saturation fluorometric analysis and atomic force microscopy showed that the electrovalent bond between L-lysine and CA-12 acted as the main force to partition L-lysine into organic phase. Moreover, the mathematically analyzed extraction process indicated that the extractive complex was a 1:2 L-lysine • CA-12 structure. The initial extraction efficiency could reach 78.9 ± 1.2% and the total strip extraction efficiency after three strip extractions could reach 95.7 ± 1.8% by using a dilute KCl solution.

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The effect of protected methionine and lysine on milk production and composition on grass-silage based diets

Cited by 7 publications

References 0 publications

Effects of protected methionine and variable energy supply on lactational responses in dairy cows fed grass silage-based diets

Effects of protected methionine and variable energy supply on lactational responses in dairy cows fed grass silage-based diets

The effect of protected lysine and methionine on milk yield and its composition in lactating dairy cows fed grass silage-based rations

A Comprehensive Study of Extraction of L-Lysine withSec-Octylphenoxy Acetic Acid in Sulfonated Kerosene

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