The effect of proteoglycans inhibited on the neurotropic growth of salivary adenoid cystic carcinoma

Zhang, Yanning; Wang, Jie; Fang, Dong; Li, Hexiang; Hou, Yuchuan

doi:10.1111/j.1600-0714.2011.01024.x

Cited by 20 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytological examination is an important means of clinical disease diagnosis (Bromberg et al, 2007;Mosterd et al, 2008; Hao et al, 2011;Venugopal and Prasad, 2015). Exfoliated cells from blood, cerebrospinal fluid, spinal fluid, chest water and mucous liquid can provide a large amount of clinical information (including cell morphology, cell type, and cell proportion, etc.…”

Section: Nanozymes For Live Cell and Organelle Imagingmentioning

confidence: 99%

“…Exfoliated cells from blood, cerebrospinal fluid, spinal fluid, chest water and mucous liquid can provide a large amount of clinical information (including cell morphology, cell type, and cell proportion, etc. ), which can be used for cancer screening (Schiffman et al, 2000;Dillner et al, 2008), CNS hematologic malignancies (Bromberg et al, 2007) anemia diagnosis (Hao et al, 2011), and Langerhans cell granulomatosis detection (Mosterd et al, 2008).…”

Section: Nanozymes For Live Cell and Organelle Imagingmentioning

confidence: 99%

See 1 more Smart Citation

Nanozymes: A New Disease Imaging Strategy

Wang

Hong

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Nanozymes are nanomaterials with intrinsic enzyme-like properties. They can specifically catalyze substrates of natural enzymes under physiological condition with similar catalytic mechanism and kinetics. Compared to natural enzymes, nanozymes exhibit the unique advantages including high catalytic activity, low cost, high stability, easy mass production, and tunable activity. In addition, as a new type of artificial enzymes, nanozymes not only have the enzyme-like catalytic activity, but also exhibit the unique physicochemical properties of nanomaterials, such as photothermal properties, superparamagnetism, and fluorescence, etc. By combining the unique physicochemical properties and enzyme-like catalytic activities, nanozymes have been widely developed for in vitro detection and in vivo disease monitoring and treatment. Here we mainly summarized the applications of nanozymes for disease imaging and detection to explore their potential application in disease diagnosis and precision medicine.

show abstract

Section: Nanozymes For Live Cell and Organelle Imagingmentioning

confidence: 99%

Section: Nanozymes For Live Cell and Organelle Imagingmentioning

confidence: 99%

Nanozymes: A New Disease Imaging Strategy

Wang

Hong

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…However, it is not clear what the role of neoplastic myoepithelial cells secreting proteoglycan is. A few years ago, the Wang research group revealed that the XT-I gene of SACC cells was silenced by RNAi, and the reduction of proteoglycans inhibited cell adhesion, neurotropic invasion, and lung metastasis [ 12 , 13 ]. Furthermore, the effect of XT-I gene silencing on the implanting growth of SPA is not known.…”

Section: Discussionmentioning

confidence: 99%

“…The sequence of shRNA-WJ4 targeted to XT-I mRNA was: 5′-AAC AGG CAG CCC ATC AAA CCT. The sequence of shRNA-WJ4 is: 5′-GAT CCG CAG GCA GCC CAT CAA ACC TTT CAA GAC GAG GTT TGA TGG GCT GCC TGT TTT TTG TCG ACA-3′; 3′-GCG TCC GTC GGG TAG TTT GGA AAG TTC TGC TCC AAA CTA CCC GAC GGA CAA AAA ACA GCT GTT CGA-5′ [ 12 , 13 ]. The Ad-shRNA-HK (GAC TTC ATA AGG CGC ATG C) was not isogenous with any human gene.…”

Section: Methodsmentioning

confidence: 99%

Effect of Xylosyltransferase-I Silencing on Implanting Growth of Salivary Pleomorphic Adenoma

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundSalivary pleomorphic adenoma is one of the most common salivary gland tumors. It has a relatively high tendency to recur and a high risk of malignant transformation. The present study aimed to study the effect of XT-I gene silencing on the implanting growth of salivary pleomorphic adenoma.Material/MethodsPrimary cultures of SPA cells and fibroblasts from the same patient were assessed. The adenovirus vector Ad-shRNA-XT-I was constructed and transfected into SPA cells. The expression of XT-I gene and XT-I protein was detected by real-time PCR and Western blot. The contents of proteoglycans were detected. The SPA cells transfected with Ad-shRNA-XT-I (group SPA-XT-I) and Ad-shRNA-HK (group SPA-HK), as well as without transfection (group SPA), were implanted into ADM scaffold with fibroblasts and then transferred into 18 BALB/C-nu nude mice for 3 months.ResultsPrimary cultures showed SPA cells were positive for human CK and S-100 protein and the fibroblasts were positive for human vimentin. The expressions of XT-I gene and protein were decreased by 51% and 51.31%, respectively. The content of proteoglycans was reduced by 48.45%. The results of the implanting growth in vitro and in vivo of nude mice indicated that no tumors grew in the SPA-XT-I group, whereas SPA grew in groups SPA-HK and SPA positive for human α-SMA, S-100 protein, and calponin.ConclusionsXT-I gene silencing effectively inhibited the implanting growth of SPA.

show abstract

“…3,19,24,26 Proteoglycans are one of the major components of the ECM and are produced in large amounts by myoepithelial cells from salivary gland tumors. In this respect, Zhang et al 27 inhibited the synthesis of proteoglycans in an ACC cell lineage and then injected these cells into nude mice. They observed that these cells lost their ability of migration and perineural invasion.…”

Section: Discussionmentioning

confidence: 99%

Immunoexpression of α2β1, α3β1, and α5β1 Integrins in Pleomorphic Adenoma and Adenoid Cystic Carcinoma

Miguel

Pereira

Andrade

et al. 2013

Applied Immunohistochemistry &Amp; Molecular Morphology

View full text Add to dashboard Cite

The aim of the present study was to compare the expression of α2β1, α3β1, and α5β1 integrins between 28 pleomorphic adenomas (PAs) and 10 adenoid cystic carcinomas (ACCs), and investigate differences in the expression of these integrins according to histologic subtypes of ACCs. It was taken into consideration the presence or absence, distribution, and localization of integrin immunoexpression. There was immunoreactivity in the intercellular contacts of the strands, nests, and solid sheets of PAs, as well as in the luminal and nonluminal cells of the duct-like structures, with a predominant immunoexpression in the luminal cells. The immunoexpression in ACCs varied with histologic subtype of the tumor. It was verified for a tendency of absence and/or reduced expression of all integrins in the solid subtype of ACCs. In general, PAs revealed a more diffuse and remarkable immunoexpression of all studied integrins than ACCs. The reduced integrins expression in ACC may be related to a lesser degree of cell differentiation in this neoplasm. Moreover, the absence and/or reduced expression of the studied integrins in solid ACC suggest a possible role in pathogenesis and more aggressive biological behavior of this histologic subtype.

show abstract

The effect of proteoglycans inhibited on the neurotropic growth of salivary adenoid cystic carcinoma

Abstract: Xylosyltransferase-I gene of SACC cells was silenced by RNA interference technology, proteoglycans secretion was reduced and neurotropic invasion behavior of SACC was inhibited obviously.

Cited by 20 publications

References 24 publications

Nanozymes: A New Disease Imaging Strategy

Nanozymes: A New Disease Imaging Strategy

Effect of Xylosyltransferase-I Silencing on Implanting Growth of Salivary Pleomorphic Adenoma

Immunoexpression of α2β1, α3β1, and α5β1 Integrins in Pleomorphic Adenoma and Adenoid Cystic Carcinoma

Contact Info

Product

Resources

About