The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast-like cells on collagen/hydroxyapatite scaffolds

Abstract

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs.

Differentiation of Periodontal Ligament Stem Cells Into Osteoblasts on Hybrid Alginate/ Polyvinyl Alcohol/ Hydroxyapatite Nanofibrous Scaffolds

“…The impacts of the PVA, Alg/PVA, and Alg/PVA/HANPs nanofibrous on the expression of four important bone correlated gens were investigated by real-time reverse transcription (RT) polymerase chain reaction (RT-PCR) including bone morphogenetic protein 4 (BMPL), collagen type I (Col1), osteocalcin (OSC), runt-related transcription factor-2 (Runx-2), and alkaline phosphatase (ALP) (8,11). The cellular constructs were rinsed with saline buffer at predetermined time points one and 21 days after culture, and the total RNA was isolated using Qiazol reagent Kit, according to the manufacturer's protocol.…”

“…In addition, bioactive inorganic materials have guided seeded cells into aim specific cells and tissues. Among these substrates, sodium alginate, a polyelectrolyte chain with negative charges on its backbone, has been extensively used in biomedical, biopharmaceutical, and biofabrication applications, due to its relatively low cost, biocompatibility, and mild gelation behavior with divalent cations (6)(7)(8)(9). In addition, PVA is an inexpensive water-soluble polymer that has excellent physical properties and chemical stability.…”

“…In addition, hydroxyapatite (HA, Ca 10 (PO 4 ) 6 (OH) 2 ) is the main inorganic substrate through bone tissue. Nanostructured HA are extensively utilized in musculoskeletal tissue regeneration and repair due to its excellent biocompatibility and bone integration ability (7)(8)(9).…”

“…For example, PDLCs shared many osteoblast-like properties, including expression of the alkaline phosphatase as aboneassociated marker, response to bone-inductive factors, and the capacity to form mineralized nodules in in vitro. PDLCs behavior in alginate microparticle was provided as a reliable approach for bone tissue engineering and regenerative medicine (8,10,11). PDLCs in Chitosannanohydroxyapatite scaffold could induce osteogenic and adipogenic differentiation in vitro and in vivo (9).…”

“…PDLCs cells demonstrated that the porous collagen-HA, as well as gelatin-HA composites, have good biocompatibility and biomimetic properties for bone tissue regeneration. As a result of the factors mentioned above, our hypothesis is that osteogenic differentiation of the PDLCs could promote through composite scaffold, which have a desired feature as seed cells for periodontal bone regeneration in treatment of periodontal disease (8,(10)(11)(12).…”