The Effect of Qiweibaizhu Powder Crude Polysaccharide on Antibiotic-Associated Diarrhea Mice Is Associated With Restoring Intestinal Mucosal Bacteria

Abstract

BackgroundQiweibaizhu powder (QWBZP) has been shown to be effective in treating antibiotic-associated diarrhea (AAD). Previous research has reported that plant polysaccharides can promote the growth of beneficial intestinal bacteria and inhibit the multiplication of pathogenic bacteria, thus effectively treating diarrhea. Here, we investigated the effect of QWBZP crude polysaccharide on the diversity of intestinal mucosal bacteria and their community structure composition in mice with AAD, and the aim of this study was to provide the scientific basis for the efficacy of QWBZP crude polysaccharide on diarrhea.Materials and MethodsEighteen Kunming (KM) mice were randomly divided into the normal (mn) group, the model (mm) group, and the QWBZP crude polysaccharide treatment (ma) group, with six mice in each group. An AAD model was constructed using a mixed antibiotic solution and treated with gavage crude polysaccharide solution of QWBZP. The intestinal mucosa was extracted from the jejunum to the ileum of mice, and the metagenome was extracted and then analyzed using MiSeq sequencing to characterize the intestinal mucosal bacteria in mice.ResultsThe spleen and thymus indices of each group of mice had no significant differences. The Chao1 and ACE indices of the mn and mm groups were similar, the Simpson index was the largest and the Shannon index was the smallest in the mm group, and there was no significant difference in the diversity indices of all three groups. In the PCA and PCoA, the mn and ma group samples were both relatively concentrated with a high similarity of community structure. In contrast, the samples in the mm group were more scattered and farther away from the samples in the mn and ma groups, i.e., the community structure similarity within and between the groups was low. Compared with the mm group, the relative abundance of Proteobacteria, Lactobacillus, and the Firmicutes/Bacteroidetes (F/B) ratio in the ma group was decreased, while that of Enterococcus continued to increase. In the LEfSe analysis, there were significant differences in the characteristic bacteria in the mn, mm, and ma groups.ConclusionThe single crude polysaccharide component is not very effective in treating AAD, so the clinical efficacy of the QWBZP crude polysaccharide is subject to further investigation.

Seabuckthorn polysaccharide combined with astragalus polysaccharide ameliorate alcoholic fatty liver by regulating intestinal flora

“…The gut microbiota is the normal intestinal microbiota formed in the long-term evolution of the human body ( 20 ). They are distributed in the intestine with a certain regularity and form a defense barrier together with intestinal mucosa ( 21 , 22 ).…”

Effects of sweeteners on host physiology by intestinal mucosal microbiota: Example-addition sweeteners in Qiweibaizhu Powder on intestinal mucosal microbiota of mice with antibiotic-associated diarrhea

“…A mixed antibiotic solution of gentamicin sulfate injection (Yichang Renfu Pharmaceutical Co., Ltd., State Drug Quantifier H42022058, product batch number: 5120106) and cephradine capsule (Suzhou Sinochem Pharmaceutical Industry Co., Ltd., product batch number: 110804) was prepared to make a concentration of 62.5 g/L [i.e., 6 gentamicin (2 mL/branch) + 3 cephalosporins (0.25 g/grain)] and then reserved at 4 ° C ( 8 , 18 ).…”

“…The AAD model was constructed following the previous methods ( 18 – 20 ): Thirty-six mice were gavaged with an antibiotics mixture of gentamicin sulfate and cephradine (23.33 mL⋅kg –1 ⋅d –1 ) as AAD mice, six mice were gavaged with sterile water as the normal (mn) group, 0.35 mL twice daily for 5 days. When diarrheal symptoms were induced, 36 mice were randomly divided into the (mm) group, the QWBZP (mq) group, the saccharin + QWBZP (mc) group, the sucrose + QWBZP (ms) group, the xylito + QWBZP (mx) group, and the sorbitol + QWBZP (msl) group, with six mice (three males and three females) in each group.…”

“…Metagenome DNA of intestinal mucosal microorganisms was extracted according to our previous methods ( 18 , 23 ) with the following processes: acetone washing, lysozyme wall breaking, proteinase K denaturation, SDS lysis, CTAB treatment, and phenol/chloroform extraction to obtain high-quality DNA. After genomic DNA extraction, the extracted genomic DNA was detected by 1% agarose gel electrophoresis.…”

“Liver–gut” axis: A target of traditional Chinese medicine for the treatment of non-alcoholic fatty liver disease

“…A large number of studies ( 12 , 40 , 41 ) have confirmed that TCM has a positive effect on the regulation of the intestinal flora, and the destruction of the intestinal microecosystem will lead to digestive disorders, poor digestion, and damage to the microecology, further leading to digestive disorders, indigestion, anorexia, and other symptoms, consistent with the liver and kidney syndrome. The use of TCMs for strengthening the spleen and dispelling dampness, such as Chinese yam, Poria , Codonopsis , and Coix seed, improves the spleen and stomach deficiency syndrome.…”