The effect of recombinant human osteogenic protein-1 on healing of large segmental bone defects.

Cook, Stephen D.; Baffes, Gregory C.; Wolfe, Michael W.; Sampath, T. Kuber; Rueger, David C.; Whitecloud, Thomas S.

doi:10.2106/00004623-199406000-00006

Cited by 360 publications

(167 citation statements)

References 24 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…This was unexpected because the collagen carrier lacks the necessary osteoinductive peptides necessary for bone healing [9,10]. The purified bovine Type I collagen used as a carrier for OP-1 in this study was osteoconductive only [38].…”

Section: Prednisolone With Collagenmentioning

confidence: 94%

“…At present, only BMP-2 and BMP-7 are produced commercially as a therapy to enhance bone healing with BMP-7 called osteogenic protein 1 (OP-1). BMP-2 and OP-1 both promote bone formation in a variety of experimental models (rat [8,13,25,46,47,56], rabbit [3,10,29,48], sheep [15,20], dog [9,19,32,35,40,43]). Although these proteins are now used clinically, most of the information on their effects is known from these and other experimental studies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

Gilley

Wallace

Bourgeault

et al. 2009

Clin Orthop Relat Res

View full text Add to dashboard Cite

Glucocorticoids inhibit bone remodeling and fracture healing. We sought to determine whether osteogenic protein 1 (OP-1) can overcome this inhibition in a closed fracture model in the rat. Time-released prednisolone or placebo pellets were implanted subcutaneously; closed femoral fractures were created 2 weeks later in rats. Fractures received sham, OP-1 and collagen, or collagenonly implants. Femurs were harvested at 3, 10, 21, 28, and 42 days postfracture. Fractures were examined radiographically for amount of hard callus; mechanically for torque and stiffness (also expressed as a percentage of the contralateral intact femur); and histomorphometrically for amount of cartilaginous and noncartilaginous soft callus, hard callus, and total callus. Glucocorticoid administration inhibited fracture healing. The application of a devitalized Type I collagen matrix mitigated the inhibitory effects of prednisolone on fracture healing However, further increases in indices of fracture healing were observed when OP-1 was added to the collagen matrix compared with collagen alone. OP-1 and collagen was more effective than collagen alone.

show abstract

Section: Prednisolone With Collagenmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

Gilley

Wallace

Bourgeault

et al. 2009

Clin Orthop Relat Res

View full text Add to dashboard Cite

show abstract

“…The aim of this study was to test in the canine ulnar defect model, which is considered suitable for bone substitute and transplantation studies [4,8,12,15,16], whether BMP addition enhances the suitability of demineralized xenogeneic bovine bone for bone grafting and whether xenogeneic bone is a suitable carrier for BMPs.…”

Section: Introductionmentioning

confidence: 99%

Bovine bone implant with bovine bone morphogenetic protein in healing a canine ulnar defect

Tuominen

Jämsä

Tuukkanen

et al. 2001

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Xenograft is considered an alternative material for bone transplantation, but its bone healing capacity is inferior compared to that of autografts and allografts. Here, we tested whether bone morphogenetic protein (BMP) addition enhances the suitability of demineralized xenogeneic bovine bone for bone grafting in dogs, and whether xenogeneic bone is a suitable carrier material for BMPs. The capacity of demineralized bovine bone implants, with and without native partially purified bovine BMP, to heal a 2-cm ulnar defect was determined in six dogs over a follow-up time of 20 weeks. No instances of bone union were seen, but there was slightly more bone formation in the xenografts with BMP, though the difference was not statistically significant. The ulnas treated with an implant with BMP were also mechanically stronger, but the difference was not significant. Computed tomography scans showed no differences in the implant area in bone density, bone mineral content, or bone cross-sectional area. It is concluded that native, partially purified BMP does not sufficiently improve the suitability of bovine demineralized xenografts as a bone substitute material for dog. Demineralized xenogeneic bone does not seem to be a feasible carrier material for BMP.

show abstract

“…BMP-7) promotes bone formation and healing in a variety of animal models and delivery systems. [11][12][13] Like other BMPs, OP-1 acts through receptors on local connective tissue stem and progenitor cells, either as a soluble factor or a matrix bound protein, to induce these cells to undergo osteoblastic differentiation. Activation of local cells by OP-1 may also stimulate secretion of other autocrine and paracrine signals that further contribute to the local bone healing response.…”

Section: Introductionmentioning

confidence: 99%

Bone formation following OP‐1 implantation is improved by addition of autogenous bone marrow cells in a canine femur defect model

Takigami

Kumagai

Latson

et al. 2007

Journal Orthopaedic Research

View full text Add to dashboard Cite

Osteogenic Protein-1 (OP-1, BMP-7) acts locally on connective tissue progenitors (CTPs) to induce bone formation. The response to OP-1 and similar agents is potentially limited by the number of local CTPs. This study tested the hypothesis that supplementing local CTPs using autogenous bone marrow will enhance bone formation at an OP-1 implant. Four 1.0-cm diameter unicortical cylindrical defects in the left proximal femur were grafted in each of seven dogs. Radial ingrowth of new bone formation was assessed at 4 weeks using micro CT. The OP-1 (3.5 mg rhOP-1 in 1 g bovine collagen I matrix) was implanted in each site combined with either clotted blood or aspirated bone marrow (BM). Bone formation was increased in the group augmented with transplanted marrow. These data suggest that increasing the local population of cells and CTPs using aspirated bone marrow can enhance the performance of OP-1, but may not eliminate the effects of site variation on the response to OP-1 and similar agents. The canine multiple femoral defect model defined in this study is well suited to quantitatively evaluate strategies for augmenting bone repair using local cell targeting and cell transplantation strategies. ß

show abstract

The effect of recombinant human osteogenic protein-1 on healing of large segmental bone defects.

Cited by 360 publications

References 24 publications

OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

OP-1 Augments Glucocorticoid-inhibited Fracture Healing in a Rat Fracture Model

Bovine bone implant with bovine bone morphogenetic protein in healing a canine ulnar defect

Bone formation following OP‐1 implantation is improved by addition of autogenous bone marrow cells in a canine femur defect model

Contact Info

Product

Resources

About