The effect of redox potential changes on reductive dechlorination of pentachlorophenol and the degradation of acetate by a mixed, methanogenic culture

Stuart, Sheryl L.; Woods, Sandra L.; Lemmon, Teresa; Ingle, J. D.

doi:10.1002/(sici)1097-0290(19990405)63:1<69::aid-bit7>3.0.co;2-2

Cited by 26 publications

(12 citation statements)

References 35 publications

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“…In experiments using a mixed culture including methanogens, the effect of the redox potential on methanogenesis has been investigated by other researchers (Stuart et al . ). When the redox potential was increased from −0·46 V ( vs Ag/AgCl converted from SHE in the original manuscript) by the addition of H 2 O 2 and K 3 Fe(CN) 6 , methanogenesis was arrested at above −0·3 V. Sasaki et al .…”

Section: Resultsmentioning

confidence: 97%

Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus

Hirano

Matsumoto

Murakami

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: A novel bioelectrochemical method can activate the methanogenesis of M. thermautotrophicus by controlling the redox potential in culture conditions at À0Á8 V, which is a difficult potential to achieve by conventional methods (e.g. by adding reducing agents). This study provides useful insights for the application of a bioelectrochemical system in industrial processes involving methanogens, such as in anaerobic digesters. AbstractTo investigate the precise effect of the redox potential on the methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus by using an electrochemical redox controlling system without adding oxidizing or reducing agents. A bioelectrochemical system was applied to control the redox conditions in culture and to measure the methane-producing activity of M. thermautotrophicus at a constant potential from +0Á2 to À0Á8 V (vs Ag/ AgCl). Methane production and growth of M. thermautotrophicus were 1Á6 and 3Á5 times increased at À0Á8 V, compared with control experiments without electrolysis, respectively, while methanogenesis was suppressed between +0Á2 and À0Á2 V. A clear relationship between an electrochemically regulated redox potential and methanogenesis was revealed.

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Section: Resultsmentioning

confidence: 97%

Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus

Hirano

Matsumoto

Murakami

et al. 2013

Lett Appl Microbiol

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“…Agitation and temperature control were provided via a stirrer (IKA ® RCT BASIC (Stuart et al, 1999).…”

Section: Culture Conditions For Redox Potential Measurementsmentioning

confidence: 99%

“…Hence, in the presence of dechlorinating bacteria, the presence of a chlorinated solvent represents oxidative power. Highly reduced anaerobic environments, (indicated by a low redox potential) typical for methanogenesis, have been found to be a requisite for the reductive dechlorination of halogenated compounds (i.e., the substitution of halogen atoms by hydrogen atoms) (Stuart et al, 1999 (Ensley, 1991, Furukawa et al, 2005, Burris et al, 1996, Gantzer and Wackett, 1991, Glod et al, 1997and Jablonski and Ferry, 1992. These reactions have been reported to occur cometabolically or coupled to energy generating reactions where PCE serves as an electron acceptor (Fathepure et al, 1987, Gerritse et al, 1996and Holliger et al, 1993.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have shown that low redox potentials are required for dechlorination (Pardue et al, 1988, Masscheleyn et al, 1991, Schumacher et al, 1997, Stuart et al, 1999, Arnold and Roberts, 2000, Olivas et al, 2002and Shimomura and Sanford, 2005. The highest rates of dechlorination were observed at lowest redox potentials tested (E Ag/AgCl = −348 mV) ( Olivas et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The use of redox potential to monitor biochemical HCBD dechlorination

Cord‐Ruwisch

James

Charles

2009

Journal of Biotechnology

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The microbial reductive dechlorination of chlorinated solvents is a redox reaction in which the chlorinated carbon receives electrons from a suitable electron donor. Hexachloro-1,3-butadiene (HCBD) is a particularly recalcitrant chlorinated solvent. Its slow, cyanocobalamin-dependent biochemical dechlorination by mixed microbial consortia had been previously demonstrated. This study shows that the reductive dechlorination reaction of HCBD can be monitored in situ by recording the redox potential (E Ag/AgCl ). The addition of HCBD to mixed anaerobic consortia triggered a rise of E Ag/AgCl and the formation of dechlorinated endproducts. This indicated that the change in E Ag/AgCl was linked to the dechlorination of HCBD. Total concentration of dechlorinated endproducts (C4 gases) equated to approximately 50% of HCBD added. The rise and subsequent fall in E Ag/AgCl after the addition of HCBD caused a peak. Peak areas obtained, from the change in E Ag/AgCl , provided an indication of the amount of HCBD dechlorinated. Moreover, the saturation concentration of HCBD can be estimated from peak heights. This online monitoring of HCBD reductive dechlorination could potentially be used for improved process control of bioremediation reactors and on-site as online biosensors. Online monitoring of HCBD dechlorination via redox potential offers both reliability and portability at a low cost. In addition, this novel and innovative technique could potentially be used to monitor the dechlorination of contaminants other than HCBD. AbbreviationsC4 gases, dechlorinated endproducts (1,3-butadiene,1-buten-3-yne and 1,3-butadiyne); E Ag/AgCl , redox potential (referenced to Ag/AgCl electrolyte); EBCRC, environmental biotechnology co-operative research

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“…30,31 Thus, it has been shown that raising the redox potential of environments leads to a substantial decrease in methanogenic activity. 32 Conversely, the redox potential of the sediment where electrodes were not installed was lower than −150 mV (vs. SHE), indicating that the sediment was quite reduced. This may allow the production of methane as well as hydrogen sulfide because methanogenesis is responsible for the majority of the terminal carbon metabolism in freshwater sediments under anaerobic conditions.…”

Section: 27mentioning

confidence: 99%

Field Experiments on Bioelectricity Production from Lake Sediment Using Microbial Fuel Cell Technology

Hong¹,

Kim²,

Choi³

et al. 2008

Bulletin of the Korean Chemical Society

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Since particular bacteria are able to oxidize organic matter under anaerobic conditions with concomitant electricity generation while an electrode is serving as an electron acceptor in a fuel cell environment, microbial fuel cells (MFCs) have recently received significant attention. Prototype sediment MFCs were placed in a shallow hypereutrophic lake and generated current for over six months. During the experimental period, a higher electrical current density with an average of 20.4 mA/m 2 was produced using electrodes with higher specific surface areas. Furthermore, parallel connection of sediment MFCs increased the current output. The results showed that current production was severely dependent on temperature and dissolved oxygen concentrations at the cathode. Other findings of this study include not only the direct coupling of current production with a decrease in the organic matter content of the sediment, as well as high positive redox potentials (> +120 mV vs. SHE) in the vicinity of the active anode as compared to sediments where electrodes were not embedded. This implies that the electrochemically active anode evidently acted as an alternative electron acceptor in the sediment, which likely decreased the activity of methanogens and improved the overall decrease in organic matter of sediment. Consequently, this study suggests that the sediment MFC could provide a means for the bioremediation of organic-rich sediment via anaerobic oxidation in conjunction with current production.

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The effect of redox potential changes on reductive dechlorination of pentachlorophenol and the degradation of acetate by a mixed, methanogenic culture

Cited by 26 publications

References 35 publications

Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus

Electrochemical control of redox potential affects methanogenesis of the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus

The use of redox potential to monitor biochemical HCBD dechlorination

Field Experiments on Bioelectricity Production from Lake Sediment Using Microbial Fuel Cell Technology

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