The effect of replacement of fish meal with Amino Acids and Optimized Protein Levels in the diet of the Nile Tilapia Oreochromis niloticus

Abstract

This study was conducted to determine an appropriate replacement of fishmeal with amino acids (AAs) and optimized protein levels in practical diets for Oreochromis niloticus with mean initial body weight 12.52±0.63g. Six experimental and a control diet (total 7 diets) divided into two groups, and a control diet (D1) containing 32% protein. The first group contained three diets that included different dietary protein levels, viz. 20 (D2), 25 (D3), and 30% (D4) with AAs when replacing fishmeal by plant protein sources. In the second group, the diets were contained 20 (D5), 25 (D6), and 30% (D7) without AAs. The best growth performance was achieved in fish fed with diet D1. Total feed intake was increased with an increase in dietary protein level with AAs. The specific growth rate showed a similar pattern with a significant difference between control, D4 and D7 compared to other groups. The feed conversion ratio decreased when protein levels in the diets increased. The protein efficiency ratio showed a similar performance, with a slight increase between the control diet and diets with AAs. However, insignificant differences (P>0.05) were observed between diets with and without AAs. An economic evaluation indicated that inclusion of low fishmeal in tilapia diets reduced the price/kg of diets compared to control.Este estudo foi realizado para determinar uma substituição adequada de farinha de peixe com aminoácidos (AA) e níveis otimizados de proteína em dietas práticas para Oreochromis niloticus com peso corporal inicial médio de 12,52 ± 0,63 g. Seis dietas experimentais e controle (total de 7 dietas) divididas em dois grupos, e uma dieta controle (D1) contendo 32% de proteína. O primeiro grupo continha três dietas que incluíam diferentes níveis de proteína na dieta, viz. 20 (D2), 25 (D3) e 30% (D4) com AAs ao substituir a farinha de peixe por fontes de proteína vegetal. No segundo grupo, as dietas continham 20 (D5), 25 (D6) e 30% (D7) sem AAs. O melhor desempenho de crescimento foi alcançado em peixes alimentados com dieta D1. O consumo total de ração foi aumentado com o aumento do nível de proteína na dieta com AAs. A taxa de crescimento específico mostrou um padrão semelhante com uma diferença significativa entre o controle, D4 e D7 em comparação com outros grupos. A taxa de conversão alimentar diminuiu quando os níveis de proteína nas dietas aumentaram. A taxa de eficiência protéica apresentou desempenho semelhante, com discreto aumento entre a dieta controle e as dietas com AAs. Entretanto, diferenças insignificantes (P> 0,05) foram observadas entre dietas com e sem AAs. Uma avaliação econômica indicou que a inclusão de farinha de peixe baixa em dietas de tilápia reduziu o preço / kg de dietas em relação ao controle.Palavras-chave: suplementação de aminoácidos, avaliação econômica, desempenho de crescimento, ingredientes vegetais.Abdel-Warith, A-W et al.

Combination of lysine and histidine improves growth performance, expression of muscle growth‐related genes and fillet quality of grow‐out Nile tilapia

“…A series of recent studies have indicated that crystalline amino acids optimize growth and protein retention ratio in Nile tilapia (Michelato, de Oliveira Vidal, et al, 2016; Michelato, Zaminhan, et al, 2016; Nguyen et al., 2019, 2020). The key challenge regarding crystalline amino acid supplementation is associated with the rapid absorption compared to protein‐bound amino acids, impairing amino acids utilization (Abdel‐Warith et al., 2019; Brezas & Hardy, 2020). Whit this mind, fish were fed four times daily in the current study to avoid impaired amino acid pools in the plasma and minimize crystalline amino acids lixiviation of feeds (Abdel‐Warith et al., 2019).…”

“…The key challenge regarding crystalline amino acid supplementation is associated with the rapid absorption compared to protein‐bound amino acids, impairing amino acids utilization (Abdel‐Warith et al., 2019; Brezas & Hardy, 2020). Whit this mind, fish were fed four times daily in the current study to avoid impaired amino acid pools in the plasma and minimize crystalline amino acids lixiviation of feeds (Abdel‐Warith et al., 2019).…”

Effects of Dietary Protein Level on the Gut Microbiome and Nutrient Metabolism in Tilapia (Oreochromis niloticus)

“…In recent years, researchers and farmers are constantly striving to improve the protein utilization and feed efficiency of diets in order to promote healthy farmed tilapia [7][8][9]. For example, the research on protein requirement of tilapia at different stages, the analysis of the utilization of different protein sources, analysis of the effect of different protein sources and different protein level on the digestive system, the development of various additives to improve the efficiency of protein utilization, and the in-depth analysis of tilapia response to feed protein at the molecular level [10][11][12][13][14].…”

EFFECT OF GRADED LEVELS OF DIETERY PLANT PROTEIN ON ELEMENTAL CONCENTRATION OF HYBRID (Catla catla? x Labeo rohita?) FROM PAKISTAN

“…It is to be noted that Aquaculture required replacement of fish meal in the preparation of fish diets, especially for non-carnivorous species because of increasing cost day by day. Therefore, in lodge to prepare economic diets, alternative plant protein sources such as grains, pulses and oilseeds are widely used [1]. Positive impacts of good compounds in fish are badly impressed by the presence of toxic compounds in water body and fish body and also have an adverse consequence on human health when humans consume such fishes according to [2].…”