The Effect of Rhodamine-Derived Superparamagnetic Maghemite Nanoparticles on the Motility of Human Mesenchymal Stem Cells and Mouse Embryonic Fibroblast Cells

Abstract

Nanoparticles have become popular in life sciences in the last few years. They have been produced in many variants and have recently been used in both biological experiments and in clinical applications. Due to concerns over nanomaterial risks, there has been a dramatic increase in investigations focused on safety research. The aim of this paper is to present the advanced testing of rhodamine-derived superparamagnetic maghemite nanoparticles (SAMN-R), which are used for their nontoxicity, biocompatibility, biodegradability, and magnetic properties. Recent results were expanded upon from the basic cytotoxic tests to evaluate cell proliferation and migration potential. Two cell types were used for the cell proliferation and tracking study: mouse embryonic fibroblast cells (3T3) and human mesenchymal stem cells (hMSCs). Advanced microscopic methods allowed for the precise quantification of the function of both cell types. This study has demonstrated that a dose of nanoparticles lower than 20 µg·cm−2 per area of the dish does not negatively affect the cells’ morphology, migration, cytoskeletal function, proliferation, potential for wound healing, and single-cell migration in comparison to standard CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate). A higher dose of nanoparticles could be a potential risk for cytoskeletal folding and detachment of the cells from the solid extracellular matrix.

The Effect of Rhodamine B on the Cerebellum and Brainstem Tissue of Rattus Norvegicus

“…This labeling method has a better photostability compared to fluorochromes such as Cy3 labeled annexin V and offered a promising approach for tumor-related research, 8 2) The new design of eight half iridium (Ir) and ruthenium (Ru) sandwich compliments fluorescing by introducing Rhodamine derivatives into the N ^ N binding ligand features bioimaging and anticancer agents, 9 3) Rhodamine-derived superparamagnetic maghemite nanoparticles (SAMN-R) shows relatively high absorption by mammalian cells, excellent ad long term stability in intracellular space of rhodamine fluorescence shells with specific pH. SAMNR was previously tested on rat stem cells, rabbits and humans, and it is a strong cell marker, long-term stable investigation for precipitation of lysosomes, that do not affect nuclei, and with theranostic potential, 10 4) Fluorescent properties of rhodamine coated nanorods were studied using spectrometry and confocal microscopy. The photoluminescence spectrum of rhodamine functioned by HMMSN nanorods shows that excitation has a maximum located at λmax ex = 560 nm and peak emission at λexc em = 580 nm according to excitation/fluorescence emission.…”

Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

“…The quantitative parameters analyzed are relative width (%), area (%), and normalized cell density (%) inside the wound [79,80].…”