The effect of riboflavin and ultraviolet light on the infectivity of arboviruses

Abstract

Our study demonstrates that treatment of PLT concentrates with PRT (Mirasol) reduces the infectious levels of RRV, BFV, and MVEV. The relevance of the level of reduction required to prevent disease transmission by transfusion has not been fully defined and requires further investigation. In the face of a changing climate, with its associated threat to blood safety, PRT represents a proactive approach for maintaining blood safety.

Inactivation of chikungunya virus in blood components treated with amotosalen/ultraviolet A light or amustaline/glutathione

“…The CHIKV inactivation reported in this study also was significantly greater than the inactivation achieved by the treatment with UV/riboflavin in platelets (2.1 log 10 ) or plasma (2.2 log 10 ) . Interestingly, similar ranges of approximately 2.2 to 2.5 log 10 reduction have been reported for the inactivation of other arboviruses using UV/riboflavin . The ability of UV/riboflavin to inactivate CHIKV was shown to be affected by the presence of plasma proteins, something not observed for the amotosalen/UVA system …”

First reported case of transfusion‐transmitted Ross River virus infection

“…Pathogen reduction technology (PRT) is an alternative risk‐management option that would not have an impact on supply. The Blood Service is investigating the effectiveness of PRT for the prevention of arboviral transfusion transmission, including RRV, but further research is needed 10 …”

Dengue and chikungunya viruses in plasma are effectively inactivated after treatment with methylene blue and visible light

“…Standard techniques and methods for culturing mammalian and insect cell lines were used as described previously . Briefly, African green monkey kidney cells (Vero; ATCC# CCL‐81) were cultured in reduced serum medium (Opti‐MEM, Thermofisher) with 3% heat‐inactivated fetal bovine serum (FBS, Sigma‐Aldrich) at 37°C/5% CO 2 .…”