The effect of ribosomal protein S1 from Escherichia coli and Micrococcus luteus on protein synthesis in vitro by E. coli and Bacillus subtilis

Farwell, Mary A.; Roberts, Mark W.; Rabinowitz, Jesse C.

doi:10.1111/j.1365-2958.1992.tb02205.x

Cited by 59 publications

(42 citation statements)

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“…31) Inactivation of ypfD, which encodes the S1 homologue in B. subtilis, 32) also caused a considerable decrease in sporulation at high temperature (Table 3). Sorokin et al 33) reported previously that ypfD (designated it as the gene jofD) is expressed but is not essential in B. subtilis.…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling of Translation-associated Genes in SporulatingBacillus subtilisand Consequence of Sporulation by Gene Inactivation

Ohashi

Inaoka

Kasai

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Expression Profiling of Translation-associated Genes in SporulatingBacillus subtilisand Consequence of Sporulation by Gene Inactivation

Ohashi

Inaoka

Kasai

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…4) (8, 32). Therefore, rates of initiation of translation of mRNAs from all three operons will likely be low (8,32), and thus levels of the proteins encoded by these operons will also be low. Consequently, it FIG.…”

Section: Vol 188 2006mentioning

confidence: 99%

Transcription of the Bacillus subtilis gerK Operon, Which Encodes a Spore Germinant Receptor, and Comparison with That of Operons Encoding Other Germinant Receptors

Igarashi

Setlow

2006

J Bacteriol

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The gerA, gerB, and gerK operons, which encode germinant receptors in spores of Bacillus subtilis, were transcribed only in sporulation, and their mRNA levels peaked initially ϳ3 h before the initiation of accumulation of the spore's dipicolinic acid. After a rapid fall, levels of these mRNAs peaked again ϳ5 h later. In one wild-type strain (PS832), gerA mRNA was the most abundant, with levels of gerB and gerK mRNAs ϳ50% of that of gerA mRNA, whereas gerB mRNA was the most abundant in another wild-type strain (PY79). The synthesis of gerK mRNA in sporulation was abolished by loss of the forespore-specific RNA polymerase sigma factor, G , and induction of G synthesis in vegetative cells led to synthesis of gerK mRNA. SpoVT, a regulator of G -dependent gene expression, repressed gerK expression. The gerK promoter showed sequence similarities to G -dependent promoters, and deletion of elements of this putative promoter abolished gerK expression in sporulation.Spores of Bacillus species formed in sporulation are metabolically dormant and extremely resistant to environmental stresses (17, 27). As a consequence, these spores can survive for extremely long periods in the absence of nutrients (12,17,27). However, the spores can respond to nutrients in their environment, and when appropriate nutrients are present, the spore can rapidly return to life via spore germination followed by outgrowth (16,23,26). Spores sense nutrients via germinant receptors located in the spore's inner membrane (10,22). In Bacillus subtilis there are currently three known functional germinant receptors encoded by the gerA, gerB, and gerK operons, each of which contain three cistrons (16,23,26), and the three proteins encoded by each of these operons likely interact to form a functional germinant receptor (11,20). The GerA receptor responds to L-alanine alone, while the GerB receptor is required for the spore's response to a mixture of glucose, fructose, and K ϩ (GFK) plus either L-alanine or L-asparagine (16,23,26). The precise function of the GerK receptor is not known, but GerK is essential for the response of the GerB receptor to alanine or asparagine and GFK and for the stimulation of the GerA receptor's response to glucose (1,16,23,26). It has been suggested that GerK's major function is to respond to glucose (1).The regulation of expression of the gerA and gerB operons in B. subtilis was initially studied using transcriptional lacZ fusions to these operons (6, 9). These lacZ fusions are expressed only during sporulation in the developing forespore several hours before the forespore begins to accumulate its large depot (ϳ10% of spore dry weight) of pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]). Transcription of the gerA and gerB operons is largely (the gerA operon) or exclusively (the gerB operon) by RNA polymerase with the forespore-specific sigma factor, G (6, 7, 9, and see below), and the Ϫ10 and Ϫ35 sequences in the promoters of the gerA and gerB operons conform to those in G -dependent promoters. Studies of genes expressed ...

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“…The problem of overexpression is particularly severe when genes from Gram-positive bacteria are cloned in a Gram-negative host. Gram-positive bacteria use ribosomal binding sequences which are close to the consensus sequence for efficient translation initiation (Farwell et al, 1992 ;Isono & Isono, 1976). This can be avoided, or at least reduced, by using a lowcopy-number vector, in particular when a large-insert library is required to provide a scaffold of the entire bacterial genome.…”

Section: Large-insert Librariesmentioning

confidence: 99%

Cloning and assembly strategies in microbial genome projects

Frangeul

Nelson²,

Buchrieser

et al. 1999

Microbiology

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The effect of ribosomal protein S1 from Escherichia coli and Micrococcus luteus on protein synthesis in vitro by E. coli and Bacillus subtilis

Cited by 59 publications

References 45 publications

Expression Profiling of Translation-associated Genes in SporulatingBacillus subtilisand Consequence of Sporulation by Gene Inactivation

Expression Profiling of Translation-associated Genes in SporulatingBacillus subtilisand Consequence of Sporulation by Gene Inactivation

Transcription of the Bacillus subtilis gerK Operon, Which Encodes a Spore Germinant Receptor, and Comparison with That of Operons Encoding Other Germinant Receptors

Cloning and assembly strategies in microbial genome projects

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