The effect of risk factors of sheep flock management practices on the development of anthelmintic resistance in the Czech Republic

Vadlejch, Jaroslav; Kopecký, Oldřich; Kudrnáčová, M.; Čadková, Zuzana; Jankovská, Ivana; Langrová, Iva

doi:10.1016/j.smallrumres.2014.01.003

Cited by 13 publications

(9 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PT coproculture revealed that only larvae of H. contortus were identified in sheep of Charkhi Dadri. The presence of only H. contortus larvae was also reported by Vadlejch et al, (2014) in Czech Republic and Suresh (2016) at Hisar. The PT coproculture in goats of Charkhi Dadri revealed the predominance of Strongyloides sp.…”

Section: Resultssupporting

confidence: 69%

Efficacy of Ivermectin against Gastrointestinal Nematodes in Small Ruminants of Unorganized Sector in Haryana

nka¹,

Vohra²,

Singh³

2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 69%

Efficacy of Ivermectin against Gastrointestinal Nematodes in Small Ruminants of Unorganized Sector in Haryana

nka¹,

Vohra²,

Singh³

2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…The control of parasitic nematodes in small ruminants relies largely on the use of anthelmintics [ 4 – 7 ]. However, anthelmintic resistance in GINs of small ruminants have been increasingly reported worldwide [ 5 , 6 , 8 – 10 ], and is severely threatening the potential utilisation of this control strategy [ 3 , 7 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Field evaluation of the efficacy of common anthelmintics used in the control of gastrointestinal nematodes of sheep in Dabat district, Northwest Ethiopia

et al. 2017

View full text Add to dashboard Cite

BackgroundGastrointestinal nematode (GIN) infections are the main impediments that restrict the welfare and productivity of small ruminant in the world. Effective management of GINs in grazing sheep relies heavily on the use of highly efficacious anthelmintic drugs. However, anthelmintic resistance is becoming a significant concern in the world, and this phenomenon severely threatens the potential utilisation of this control strategy. Therefore, this study was conducted 1) to evaluate the efficacy of commonly used anthelmintic on GINs in naturally infected sheep and 2) to assess the farmers’ perception on anthelmintics utilisation practices in Dabat district, Northwest Ethiopia.MethodsOne hundred twenty nematode infected sheep were used in this study. Sheep were selected based on the egg count (≥150 eggs per gram of faeces). The animals were allocated randomly into four groups (30 animals per group). Group-I, II and III were treated with Albendazole, Tetramisole, and Ivermectin, respectively. The 4th group was left untreated (as control). Faecal samples were collected on day 0 (before treatment), on day 3, 7, 10 and 14 (post-treatment). The modified McMaster technique was used for quantifying the eggs. Faecal egg count reduction test (FECRT) was applied to determine the efficacy of anthelmintic at day 14 (post-treatment). In addition, a questionnaire survey was conducted on 100 randomly selected sheep owners.ResultsAll anthelmintics tested revealed significant (P < 0.05) reduction in nematode egg excretion in the sheep post-treatment. Faecal egg count reduction (FECR) levels for Albendazole, Tetramisole, and Ivermectin were 97.2, 98.9 and 97.7%, respectively. Post-treatment egg counts and percentage reduction of egg counts were not significantly different (P > 0.05) among the treatment groups. The nematode genera identified before treatment were Haemonchus, Trichostrongylus, Cooperia, Trichuris, Teladorsagia, Bunostomum, and Strongyloides. Haemonchus and Trichostrongylus were detected after treatment with Albendazole and Ivermectin. The questionnaire survey revealed that Albendazole was the most commonly (90%) used anthelmintic to treat nematodes in sheep, followed by Tetramisole (36%) and Tetraclozan (Tetramisole-Oxyclozanide combination) (20%). Respondents expressed that anthelmintic selection was made based on veterinarian prescription (84%), colour (27%), efficacy (4%), price affordability (1%) and availability (1%).ConclusionThis study demonstrated that the tested anthelmintics had an acceptable level of efficacy against GINs of sheep.

show abstract

“…PGE in ruminants is usually caused by a mixture of GINs occurring simultaneously [ 3 , 33 ]. Therefore, the potential implications for studying these parasites (population biology, epidemiology, treatment efficacy, etc.)…”

Section: Discussionmentioning

confidence: 99%

The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

et al. 2021

View full text Add to dashboard Cite

Background The diagnosis of gastrointestinal nematode (GIN) infections in ruminants is routinely based on morphological/morphometric analysis of parasite specimens recovered by coprological methods, followed by larval culture (LC) techniques. Such an approach is laborious, time-consuming, requires a skilled expert, and moreover suffers from certain limitations. Molecular tools are able to overcome the majority of these issues, providing accurate identification of nematode species and, therefore, may be valuable in sustainable parasite control strategies. Methods Two multiplex real-time polymerase chain reaction (PCR) assays for specific detection of five main and one invasive GIN species, including an internal amplification control to avoid false-negative results, were designed targeting SSU rRNA and COI genetic markers, as well as established ITS1/2 sequences. The assays were optimized for analysis of DNA extracted directly from sheep faeces and verified for Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis, Nematodirus battus, Chabertia ovina, and Ashworthius sidemi. Semi-quantitative evaluation of infection intensity was enabled using a plasmid construct and a dilution series of sheep faeces with a known number of nematode eggs. Assays were tested on 44 individually collected faecal samples from three farms, and results were compared to those from faecal egg counts (FEC) using the concentration McMaster technique and LC. Results Multiplex real-time PCR assays showed great specificity to target nematodes. During the analysis of faecal samples, the assays proved to have higher sensitivity in strongylid-type egg detection over FEC by revealing three false-negative samples, while showing moderate agreement in evaluation of infection intensity. The multiplex assays further clarified GIN species identification compared to LC, which had confused determination of Teladorsagia spp. for Trichostrongylus spp. Conclusions Our multiplex assays proved to be a rapid and accurate approach enabling simultaneous and reliable GIN species identification from faeces and semi-quantitative estimation of the number of eggs present. This approach increases diagnostic value and may add a high degree of precision to evaluation of anthelmintic efficacy, where it is important to identify species surviving after treatment. Graphical Abstract

show abstract

The effect of risk factors of sheep flock management practices on the development of anthelmintic resistance in the Czech Republic

Cited by 13 publications

References 51 publications

Efficacy of Ivermectin against Gastrointestinal Nematodes in Small Ruminants of Unorganized Sector in Haryana

Efficacy of Ivermectin against Gastrointestinal Nematodes in Small Ruminants of Unorganized Sector in Haryana

Field evaluation of the efficacy of common anthelmintics used in the control of gastrointestinal nematodes of sheep in Dabat district, Northwest Ethiopia

The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

Contact Info

Product

Resources

About