The effect of saliva on the penetration of fluorescent dyes into the oral mucosa of the rat and rabbit

Role of salivary mucins in the protection of the oral cavity

“…Such treatment also permits the//i vivo penetration of benzopyrene through rat keratinized tnueosa (Wallenius 1966). Sitnilar findings have been deseribed by the in vivo studies of Adams (1974bAdams ( , 1975 who observed penetration of the dye acridine orange through nonkeratinized rabbit buceal mucosa following inhibition of salivary flow with scopolamine. In addition, Adams (1975) found that pretreatment of the buceal mucosa with tnucolytic agents enhanced dye pertneability in rabbits with uncomprotnised salivary flow.…”

Effect of Hydrogen Sulfide and Methyl Mercaptan on the Permeability of Oral Mucosa

“…The morphology of the tissue and its constant exposure to inflammatory agents and mechanical stress suggest that the oral sulcular epithelium is a weak barrier to antigenic substances, as attested to by numerous in vivo and in vitro permeability studies. Studies on permeation of substances through mucosal tissues have included measurements of permeability to [3H] -H2 0 (Kaaber, 1 973;Adams, 1974), drugs (Altman et al, 1960), topical application of antigen (Rizzo, 1970;McDougall, 1972), labeled human albumin (Tolo, 1971), horseradish peroxidase (Squier, 1973), endotoxin (Schwartz et al, 1973;Alfano et al,, 1975), inulin (Alfano et al, 1977), and dextran (Tolo and Johnsen, 1975). The initiation of periodontal disease appears to be associated with the breakdown of the integrity of epithelial basement membrane (Stern, 1965).…”

Recent advances in oral mucosal research

“…Other studies employed radiolabels or drugs such as nitroglycerin which could be systematically monitored (Tolo & Jonsen 1975). Penetration and subsequent localization of radiolabels, by autoradiography, or of fluorescent compounds by fluorescence microscopy, has also been investigated (Tolo 1971, Adams 1974a).…”