The Effect of Salt Concentration and Cation Valency on Interactions Between Mucin-Coated Hydrophobic Surfaces

Pettersson, Torbjörn; Feldötö, Zsombor; Claesson, Per M.; Dédinaité, Andra

doi:10.1007/2882_2008_075

Cited by 8 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…53 In addition, it has been suggested that a shortened Debye-length can decrease the range and magnitude of the steric repulsion forces acting between mucin layers. 54 In salt solutions, mucins are reported to undergo a conformational change from a fully extended state to a collapsed state, and, consistently, we observe a decrease of the hydrodynamic size of mucins in the presence of NaCl when probed with dynamic light scattering (Figure S4). This behavior was suggested to originate from a combination of two effects: electrostatic screening and osmotic pressure acting on the mucin chains.…”

Section: Influence Of the Mucin Concentrationsupporting

confidence: 74%

“…There are several possibilities to rationalize this observation: High ion concentrations induce Debye screening and thus reduce the number of charged groups available for ionic paring 53 . In addition, it has been suggested that a shortened Debye-length can decrease the range and magnitude of the steric repulsion forces acting between mucin layers 54 . In salt solutions, mucins are reported to undergo a conformational change from a fully extended state to a collapsed state, andconsistently, we observe a decrease of the hydrodynamic size of mucins in the presence of NaCl when probed with dynamic light scattering (Fig.…”

Section: Influence Of the Nacl Concentrationmentioning

confidence: 99%

See 1 more Smart Citation

The Lubricity of Mucin Solutions Is Robust toward Changes in Physiological Conditions

Song

Winkeljann

Lieleg

2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Abstract：Solutions of manually purified gastric mucins have been shown to be promising lubricants for biomedical purposes, where they can efficiently reduce friction and wear. However, so far, such mucin solutions have been mostly tested in specific tribological settings, i.e. in combination with different material pairings; variations in the composition of the lubricating fluid have not been systematically explored yet. We here fill this gap and determine the viscosity, adsorption behavior, and lubricity of porcine gastric mucin solutions on hydrophobic surfaces at different pH levels, mucin and salt concentrations and in the presence of other proteins. We demonstrate that mucin solutions provide excellent lubricity even at very low concentrations of 0.01 % (w/v), over a broad range of pH levels and even at elevated ionic strength. Furthermore, we provide mechanistic insights into mucin lubricity, which help explain how certain variations in physiologically relevant parameters can limit the lubricating potential of mucin solutions. Our results motivate that solutions of manually purified mucin solutions can be powerful biomedical lubricants, e.g. serving as artificial joint fluids for viscosupplementation, as eye drops or mouth spray, or as a personal lubricant for intercourse.

show abstract

Section: Influence Of the Mucin Concentrationsupporting

confidence: 74%

Section: Influence Of the Nacl Concentrationmentioning

confidence: 99%

The Lubricity of Mucin Solutions Is Robust toward Changes in Physiological Conditions

Song

Winkeljann

Lieleg

2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…Electrostatic and hydrophobic interactions play a critical role in the conformation, solvency, substrate interactions, and barrier properties of proteins 13,24,38 . Modulating pH, ionic strength, and ion valencies allows for the ionic state of the solution to be adjusted 3,12,13 , thereby allowing for control over sol-gel transitions in mucus and in mucin solutions 1,13,39 . Studies have shown that multivalent cations can affect mucin conformations even at very low concentrations (~1 mM) 1,36,39 .…”

Section: Mucin Rheological Behavior and Microstructure As A Function mentioning

confidence: 99%

“…Modulating pH, ionic strength, and ion valencies allows for the ionic state of the solution to be adjusted 3,12,13 , thereby allowing for control over sol-gel transitions in mucus and in mucin solutions 1,13,39 . Studies have shown that multivalent cations can affect mucin conformations even at very low concentrations (~1 mM) 1,36,39 . Unlike the electrostatic screening observed with monovalent ions, there is evidence that multivalent ions bind with specific anionic sites on the mucin side chains.…”

Section: Mucin Rheological Behavior and Microstructure As A Function mentioning

confidence: 99%

Chemical and Microstructural Characterization of pH and [Ca2+] Dependent Sol-Gel Transitions in Mucin Biopolymer

Curnutt

Smith

Darrow

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Mucus is responsible for controlling transport and barrier function in biological systems, and its properties can be significantly affected by compositional and environmental changes. In this study, the impacts of pH and cacl 2 were examined on the solution-to-gel transition of mucin, the primary structural component of mucus. Microscale structural changes were correlated with macroscale viscoelastic behavior as a function of pH and calcium addition using rheology, dynamic light scattering, zeta potential, surface tension, and ftiR spectroscopic characterization. Mucin solutions transitioned from solution to gel behavior between pH 4-5 and correspondingly displayed a more than tenfold increase in viscoelastic moduli. Addition of CaCl 2 increased the sol-gel transition pH value to ca. 6, with a twofold increase in loss moduli at low frequencies and tenfold increase in storage modulus. changing the ionic conditions-specifically [H + ] and [ca 2+ ]-modulated the sol-gel transition pH, isoelectric point, and viscoelastic properties due to reversible conformational changes with mucin forming a network structure via non-covalent cross-links between mucin chains. Mucin is a polyelectrolyte glycoprotein found in mucus, the structured complex fluid found in all types of organisms from bacteria to humans. In vertebrates, mucus is produced by mucus membranes and can be found lining the eyelids, mouth, and nose, as well as gastrointestinal, respiratory, and genital tracts. Since the 1970s 1 , animal mucus and mucin solutions have been well studied, especially the roles mucus plays in drug delivery 2-6 , disorders like cystic fibrosis 7,8 , and its protective biological functions 9-12. Mucin glycoproteins are present in mucus at concentrations of 1-5%, along with electrolytes (ca. 1%), lipids (1-2%), other proteins (1-2%), and water (90-95%) 13. Despite being present in low concentrations, mucin glycoproteins are primarily responsible for the protective and lubricative functions of mucus within the body. Solubilized mucin behaves as a complex fluid with changing viscoelastic and structural properties in response to its environmental conditions. Mucin glycoproteins are responsible for the majority of the physical properties of mucus, as mucin conformation, intra-and inter-strand bonding, and microstructure changes in response to environmental factors such as pH 6,7,12,14 , temperature 2,15 , and ion content 1,14,16. All these factors affect the reversible supramolecular bonding between functional groups within the protein strands, which modifies the microstructure as well as the mechanical and transport properties of the mucin network. In aqueous solutions, biopolymers like mucin form networks that can change dynamically due to non-covalent crosslinks, including physical entanglements and supramolecular bonding. Mucins resemble a bottle brush polymer with a protein backbone and oligosaccharide (carbohydrate) side chains arranged radially from the backbone. The protein backbone of the mucin biopolymer has areas that are dense w...

show abstract

“…Recently, efforts have been made towards constructing systems where certain interactions can be triggered by physical means such as temperature or light [1][2][3][4] or by chemical means like pH or (specific) salts. [5][6][7] In particular, systems containing surfaces coated with temperature-and pH-sensitive polymers have been of great interest. 2,3,[8][9][10] Swelling or collapsing polymer layers might lead to more long-range/short-range steric repulsion and polymer bridging.…”

Section: Introductionmentioning

confidence: 99%

Tuning structural forces between silica surfaces by temperature-induced micellization of responsive block copolymers

Thormann

Claesson

Mouritsen

2010

Phys. Chem. Chem. Phys.

Self Cite

View full text Add to dashboard Cite

The aim of this study is to demonstrate a method to control interactions between two surfaces by the use of a responsive solution. This was done by performing AFM-based force measurements between two silica surfaces immersed in an aqueous solution of thermo-responsive Pluronics P85 block copolymers. For this system we demonstrate that one can switch between a situation where no long-range forces are acting between the surfaces to a situation where a long-range structural force, oscillating between attractive and repulsive force regimes, is controlling the surface interaction. This shift in behavior is caused by a long-range order introduced by temperature-induced micellization of the block copolymers and is thus simply achieved by changing the temperature from below to above the micellization temperature. We propose that the use of micelle-forming responsive block copolymers is a general method for reversibly controlling surface interactions by a temperature switch. Since the force control is achieved by changes occurring in bulk solution, the nature of the surfaces is expected to play only a relative minor role.

show abstract

The Effect of Salt Concentration and Cation Valency on Interactions Between Mucin-Coated Hydrophobic Surfaces

Cited by 8 publications

References 29 publications

The Lubricity of Mucin Solutions Is Robust toward Changes in Physiological Conditions

The Lubricity of Mucin Solutions Is Robust toward Changes in Physiological Conditions

Chemical and Microstructural Characterization of pH and [Ca2+] Dependent Sol-Gel Transitions in Mucin Biopolymer

Tuning structural forces between silica surfaces by temperature-induced micellization of responsive block copolymers

Contact Info

Product

Resources

About