The effect of selecting oocytes for insemination and transferring all resultant embryos without selection on outcomes of assisted reproduction

Sala, Giovanni Battista La; Nicoli, Alessia; Villani, Maria Teresa; Girolamo, Roberta Di; Capodanno, Francesco; Blickstein, Isaac

doi:10.1016/j.fertnstert.2007.11.010

Cited by 14 publications

(13 citation statements)

References 9 publications

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“…(2009)CP−−−−−−−Yes*−*Length, retardation: yes; position: noKonc et al . (2004)F, CP−−−−−−−−Yes*−*Presence for CP onlyLa Sala et al . (2009)F, PR, THB, MB++++−++−−NoLin et al .…”

Section: Resultsmentioning

confidence: 99%

“…Unfortunately the majority (27) of the papers restricted the outcome investigation to the in vitro period, with limited value regarding the primary outcome including clinical pregnancy, implantation and ongoing pregnancy rates. Only three publications (Montag et al ., 2008; Rienzi et al ., 2008; La Sala et al ., 2009) extended the investigation to the ultimate parameter, the number of take home babies. Moreover, because of the heterogeneity of the approaches and the unavailability of clinical data, the correlation between oocyte morphology and patient and cycle characteristics was not included in the analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, at present, they can only be regarded as diverse approaches to the same strategy, but their comparative evaluation is impossible. Paradoxically, two recent publications investigating similar parameters and extending the outcome evaluation to take home babies (Rienzi et al ., 2008; La Sala et al ., 2009) resulted in contradicting conclusions about the predictive value of their system.…”

Section: Discussionmentioning

confidence: 99%

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Predictive value of oocyte morphology in human IVF: a systematic review of the literature

Rienzi

Vajta

Ubaldi

2010

Human Reproduction Update

255

196

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BACKGROUNDNon-invasive selection of developmentally competent human oocytes may increase the overall efficiency of human assisted reproduction and is regarded as crucial in countries where legal, social or religious factors restrict the production of supernumerary embryos. The purpose of this study was to summarize the predictive value for IVF success of morphological features of the oocyte that can be obtained by light or polarized microscopic investigations.METHODSStudies about oocyte morphology and IVF/ICSI outcomes were identified by using a systematic literature search.RESULTSFifty relevant articles were identified: 33 analysed a single feature, 9 observed multiple features and investigated the effect of these features individually, 8 summarized the effect of individual features. Investigated structures were the following: meiotic spindle (15 papers), zona pellucida (15 papers), vacuoles or refractile bodies (14 papers), polar body shape (12 papers), oocyte shape (10 papers), dark cytoplasm or diffuse granulation (12 papers), perivitelline space (11 papers), central cytoplasmic granulation (8 papers), cumulus–oocyte complex (6 papers) and cytoplasm viscosity and membrane resistance characteristics (2 papers). None of these features were unanimously evaluated to have prognostic value for further developmental competence of oocytes.CONCLUSIONSNo clear tendency in recent publications to a general increase in predictive value of morphological features was found. These contradicting data underline the importance of more intensive and coordinated research to reach a consensus and fully exploit the predictive potential of morphological examination of human oocytes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Predictive value of oocyte morphology in human IVF: a systematic review of the literature

Rienzi

Vajta

Ubaldi

2010

Human Reproduction Update

255

196

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“…Oocyte morphological evaluation before ICSI helps to identify metaphase II (MII) oocytes appropriate for sperm injection which show increased developmental capacity. Such evaluation includes comprises variations of oocyte form, color, granularity and homogeneity of cytoplasm, width of perivitelline space, debris in the perivitelline space, vacuolization, inclusions and abnormalities of the first polar body (1 st PB) or of the ZP (Wilding et al, 2007;La Sala et al, 2009;Levi et al, 2013).…”

Section: Oocyte Assessmentmentioning

confidence: 99%

Charting new frontiers in In Vitro Fertilization (IVF): The Role of Bioengineering

Carneiro

Lamaita

Barbosa

et al. 2015

JBRA Assisted Reproduction

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Since the beginning of in vitro fertilization (IVF) 36 years ago, scientists have studied and critically analyzed the techniques in order to find ways to improve outcomes. However, success rates vary significantly among clinics due to poor reproducibility and inconsistency across operators. Much research has been conducted on the chemical environment, or culture medium, surrounding the oocyte/ embryo, but little attention has been given to the actual equipment and physical culture environment, which has changed very little over the years. The aim of this paper was to evaluate how the physical factors are important regulators of oocyte and embryo function and to improve understanding of the physical forces involved in the processes in human reproduction. A review the available literature was conducted using PubMed from 1966 through July 2014 in an attempt to help integrate mechanics into our understanding of the molecular basis of IVF. Keywords included in vitro fertilization, biomechanics, bioengineering, oocyte and embryo. The mechanical characterization of oocytes and embryos represents an opportunity to detect cellular defects, assess quality and bio-viability of processes such as cryopreservation as well as select the best embryo for transfer. Defining the mechanical forces at play during embryo transfer is also an important step towards improving results in in vitro fertilization. The further analysis of these phenomena needs a detailed monitoring of the mechanical conditions and more extensive studies of events on the cellular and molecular levels.

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“…[6,7] Morfolojik açıdan bakıldığında sitoplazmik granül, fragmanlı PB ve sitoplazmik vakuol varlığı oosit kalitesini bozmaktadır. [6,8] Fragmente PB'li oositlerin fertilizasyon oranlarının, normal görünümlü PB'li oositlerden daha düşük olduğu ve sitoplazmik yetersizliğe işaret ettiği düşünülmektedir. [9] Oosit boyutu ve komponent oranlarının, oosit kalitesi adına ipucu verebileceği ileri sürülmektedir.…”

Section: Introductionunclassified

Impact of oocyte morphological components on fertilization

Özkan¹,

Ekinci²,

Timurkan³

et al. 2014

J Kartal TR

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ÖzetAmaç: Polar cisimcik (PB) büyüklüğü, zona pellusida (ZP) kalınlığı ve oosit sitoplazma (OS) çapının fertilizasyon üzerine etkisi olup olmadığını araştırmak. Gereç ve Yöntem:Bu ileriye yönelik randomize çalışmaya tubal faktör etiyolojisi ile intrasitoplazmik sperm injeksiyonu (ICSI) uygulanan 224 matür oosit dahil edildi. ICSI esnasında mikroskop altında PB'nin yatay ve dikey çapları (mikrometre), ZP'nin saat 3-6-9-12 hizalarındaki kalınlıkları (mikrometre) ve oosit sitoplazmasının yatay ve dikey çapları (mm) ölçülüp aritmetik ortalamaları hesaplandı. ICSI'dan 24 saat sonra pronükleus oluşumuna gore fertilizasyon kontrolü yapıldı. Bulgular:Çalışmadaki olguların ortalama yaş, vücut kitle indeksi, antral folikül sayısı, matür oosit sayısı ve stimülasyon süresi sırasıyla 31.5±4.2 yıl, 26.5±4.7 kg/m 2 , 12.9±4.5, 13±3.8 ve 8.1±0.9 gün idi. Ortalama PB çapı, ZP kalınlığı ve OS çapı sırasıyla 6.1±1.1 mcm, 6.4±0.5 mcm and 36.7±1.9 mm idi. ROC analizinde ZP kalınlığı için AUC= 0.47, LR+= 2.4 ve 7.275 mcm cut-off kabul edildiğinde fertilizasyon %93.7 spesifite ve %6 sensitivite ile öngürebilir. Regresyon analizinde sadece ZP kalınlığı (RR: 5.3, %95 GA: 1.85-15.19, p=0.002) fertilizasyon oranı üzerine etkili bulundu. Sonuç:Oosit morfolojik komponentlerinden ZP kalınlığının fertilizasyon oranı üzerine etkisi olduğunu gözledik.

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The effect of selecting oocytes for insemination and transferring all resultant embryos without selection on outcomes of assisted reproduction

Cited by 14 publications

References 9 publications

Predictive value of oocyte morphology in human IVF: a systematic review of the literature

Predictive value of oocyte morphology in human IVF: a systematic review of the literature

Charting new frontiers in In Vitro Fertilization (IVF): The Role of Bioengineering

Impact of oocyte morphological components on fertilization

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