The effect of serum proteins on apatite growth for 45S5 Bioglass and common sol-gel derived glass in SBF

Abstract

Abstract:The inhibitive effects of serum proteins on apatite growth was compared between melt-derived 45S5 Bioglass and sol-gel derived bioactive glass of the 70S30C (70 mol% SiO 2 , 30 mol% CaO). By using techniques of XRD, TEM and Raman spectroscopy, the transformation of amorphous calcium phosphate to crystalline apatite, and the resulting size and aspect ratio of the crystals, in simulated body fluid (SBF), was seen to decrease in the presence of serum. XRD showed more rapid HA formation on Bioglass particles, compared to that forming on 70S30C particles, however TEM showed similar size and frequency of the needle-like crystals. Phosphate reduction in SBF was similar for Bioglass and 70S30C. Calcium carbonate formation was more likely on the phosphate-free sol-gel glass than on Bioglass.

Strontium-releasing mesoporous bioactive glasses with anti-adhesive zwitterionic surface as advanced biomaterials for bone tissue regeneration

“…In addition, the high number of terminal hydroxyl groups allows to easily functionalise MBG surface by grafting alkoxysilane moieties with the aim to achieve several targets, such as improved drug loading ability, the reduction of particle aggregation and nonspecific surface adhesion. In this regard, it is well known that the formation of nonspecific protein layer on the surface of devices contacted with biological fluids can heavily reduce their performance, due to the inhibitive effect on drug/ion release and on the formation of apatite layer [20], essential for a strong bond with living bone tissue and the promotion of osteogenesis. The adsorption of serum proteins has also a role on the microbial adhesion and biofilm formation [21,22], as the initial attachment of individual bacterial cells or small bacterial aggregates, is usually preceded by the adsorption of biological macromolecules [11,23], such as proteins.…”

Comparative evaluation of incorporation calcium silicate and calcium phosphate nanoparticles on biomimetic dentin remineralization and bioactivity in an etch-and-rinse adhesive system

“…The ACP NPs changed to a semi-crystalline structure, as the HA growth phase was observed in the structure( 19 ). In samples containing CS NPs, calcium ions from cation exchange with hydrogen ions of the coating, silica-rich gel-like layer on the NP surface, and oversaturated calcium were removed from the coating ( 21 ).…”

Electrophoretic Deposition of 58S Bioactive Glass- Polymer Composite Coatings on 316L Stainless Steel: An Optimization for Corrosion, Bioactivity, and Cytocompatibility

“…59 The HAp growth is characterized by the signature peaks of P−O bonds at 435, 590, 960, and 1075 cm −1 . 60,61 The HAp growth that occurs over EC6 coating after 7 days of SBF immersion is indicated by a peak shift (950 to 960 cm −1 ). 61 A summary of the Raman frequencies and their respective band assignments is given in Table S3.…”

“…60,61 The HAp growth that occurs over EC6 coating after 7 days of SBF immersion is indicated by a peak shift (950 to 960 cm −1 ). 61 A summary of the Raman frequencies and their respective band assignments is given in Table S3. The surface morphology of the EC6 system and EDS for different time durations is shown in Figure 8(b−e).…”