The effect of short/branched chain acyl-coenzyme A dehydrogenase gene on triglyceride synthesis of bovine mammary epithelial cells

Ping, Jiang; Fang, Xibi; Zhao, Zhihui; Yu, Xianzhong; Sun, Boxing; Yu, Haibin; Yang, Runjun

doi:10.5194/aab-61-115-2018

Cited by 12 publications

(16 citation statements)

References 25 publications

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“…In these pathways, ACADSB, CPT2, and FABP1 were down-regulated in weaned piglets. ACADSB is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids (Jiang et al, 2018). FABP1 plays a crucial role in intracellular fatty acid transport by binding and properly targeting long-chain fatty acids to the correct metabolic sites (Samulin et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Weaning Alters Intestinal Gene Expression Involved in Nutrient Metabolism by Shaping Gut Microbiota in Pigs

et al. 2020

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Weaning transition usually impairs intestinal architecture and functions and results in gut-associated disorders in pigs. Understanding the changes in intestinal transcriptome and gut microbiota during weaning transition is important for elucidating the underlying mechanism of weaning stress. In the present study, we performed RNA-seq to determine the changes in intestinal transcriptome and 16S rRNA sequencing to measure the gut microbiota changes in the weaning transition. Transcriptome results indicated that weaning transition altered intestinal gene expression involved in nutrient transport and metabolism. Regarding fatty metabolism, fatty acid-binding protein 1 (FABP1), acyl-CoA dehydrogenase (ACADSB), and carnitine palmitoyltransferase 2 (CPT2) expression in the intestine was decreased by weaning. Genes related to bile acid metabolism were increased by weaning, including FABP6, farnesoid X receptor (FXR or NR1H4) and organic solute transporter-α (SLC51A). In addition, genes associated with oxidative stress were altered by weaning transition, including decreased catalase (CAT) and lactate dehydrogenase (LDHA) and increased glutathione peroxidase 2 (GPX2) and superoxide dismutase 3 (SOD3). Results of microbiota composition showed that the Firmicutes abundance and Firmicutes/Bacteroidetes ratio were increased and that the Proteobacteria abundance in the fecal microbiota was decreased by the weaning process; during the weaning transition, the Bacteroides and Fusobacterium abundances decreased markedly, and these bacteria nearly disappeared, while the Prevotella abundance showed a marked increase. Moreover, the levels of the microbial metabolites butyrate and acetate increased with changes in gut microbiota composition. In addition, predictive metagenome by PICRUSt analysis showed that the pathways related to D-glutamine and D-glutamate metabolism, citrate cycle (TCA cycle), peroxisome proliferators-activated receptor (PPAR) signaling, alpha-linolenic acid metabolism were decreased and the pathway related to retinol metabolism was increased in the gut microbiota of piglets during weaning transition. Our results showed that early weaning alters intestinal gene expression involved in nutrient metabolism, which may be due to the changes in microbiota composition.

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Section: Discussionmentioning

confidence: 99%

Weaning Alters Intestinal Gene Expression Involved in Nutrient Metabolism by Shaping Gut Microbiota in Pigs

et al. 2020

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“…Hence, we performed PCR array for T cell metabolic pathways (Almeida et al, 2016; Ganeshan and Chawla, 2014) and found significant upregulation in the expression of short/branched-chain specific acyl-CoA dehydrogenase ( Acadsb ) in T/NIX −/− Ova-EM (Figure 6B). ACADSB contributes to oxidation of short/branched-chain fatty acids (Alfardan et al, 2010; Jiang et al, 2018; Luís et al, 2011; Vockley et al, 2000); thus, T/NIX −/− Ova-CD8 + T cells likely exhibited a shift in fatty acid metabolism during effector memory formation. Consistent with reduced long-chain fatty acid synthesis, we observed a decrease in gene expression of long-chain acyl-CoA dehydrogenase ( Acadl ) (Figure 6B), as this enzyme carries out β-oxidation of long-chain fatty acids synthesized by FASN (Kurtz et al, 1998).…”

Section: Resultsmentioning

confidence: 99%

NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells

et al. 2019

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SUMMARY Autophagy plays a critical role in the maintenance of immunological memory. However, the molecular mechanisms involved in autophagy-regulated effector memory formation in CD8+ T cells remain unclear. Here we show that deficiency in NIX-dependent mitophagy leads to metabolic defects in effector memory T cells. Deletion of NIX caused HIF1α accumulation and altered cellular metabolism from long-chain fatty acid to short/branched-chain fatty acid oxidation, thereby compromising ATP synthesis during effector memory formation. Preventing HIF1α accumulation restored long-chain fatty acid metabolism and effector memory formation in antigen-specific CD8+ T cells. Our study suggests that NIX-mediated mitophagy is critical for effector memory formation in T cells.

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“…However, it was differentially expressed in bovine mammary epithelium (Jiang et al, ). ACADSB was more highly expressed in cows whose milk had a high milk fat content compared to those with lower milk fat (Jiang et al, ). In F‐to‐G treatment steers, ACADSB gene expression increased sharply at the end of the study; possibly, the delay in increased expression may be related to a threshold of FAs not met until the highest grain diet was provided.…”

Section: Discussionmentioning

confidence: 99%

“…ACADSB is associated with short/branched-chain FA metabolism (Rozen et al, 1994), more specifically the first step of the mitochondrial β-oxidation reaction (Ensenauer et al, 2005). However, it was differentially expressed in bovine mammary epithelium (Jiang et al, 2018). ACADSB was more highly expressed in cows whose milk had a high milk fat content compared to those with lower milk fat (Jiang et al, 2018).…”

Section: Fatty Acidsmentioning

confidence: 99%

Jersey steer ruminal papillae histology and nutrigenomics with diet changes

Novak

Rodríguez-Zas

Southey

et al. 2019

Animal Physiology Nutrition

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The transition from a high forage to a high concentrate diet is an important milestone for beef cattle moving from a stocker system to the feedlot. However, little is known about how this transition affects the rumen epithelial gene expression. This study assessed the effects of the transition from a high forage to a high concentrate diet as well as the transition from a high concentrate to a high forage diet on a variety of genes as well as ruminal papillae morphology in rumen fistulated Jersey steers. Jersey steers (n = 5) were fed either a high forage diet (80% forage and 20% grain) and transitioned to a high concentrate diet (20% forage and 80% grain) or a high concentrate diet (40% forage and 60% grain) and transitioned to a high forage diet (100% forage). Papillae from the rumen were collected for histology and RT‐qPCR analysis. Body weight had a tendency for significant difference (p = .08). Histological analysis did not show changes in papillae length or width in steers transitioning from a high forage to a high concentrate diet or vice versa (p > .05). Genes related to cell membrane structure (CLDN1, CLDN4, DSG1), fatty acid metabolism (CPT1A, ACADSB), glycolysis (PFKL), ketogenesis (HMGCL, HMGCS2, ACAT1), lactate/pyruvate (LDHA), oxidative stress (NQO1), tissue growth (AKT3, EGFR, EREG, IGFBP5, IRS1) and the urea cycle (SLC14A1) were considered in this study. Overall, genes related to fatty acid metabolism (ACADSB) and growth and development (AKT3 and IGFBP5) had a tendency for a treatment × day on trial interaction effect. These profiles may be indicators of rumen epithelial adaptations in response to changes in diet. In conclusion, these results indicate that changes in the composition of the diet can alter the expression of genes with specific functions in rumen epithelial metabolism.

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The effect of short/branched chain acyl-coenzyme A dehydrogenase gene on triglyceride synthesis of bovine mammary epithelial cells

Cited by 12 publications

References 25 publications

Weaning Alters Intestinal Gene Expression Involved in Nutrient Metabolism by Shaping Gut Microbiota in Pigs

Weaning Alters Intestinal Gene Expression Involved in Nutrient Metabolism by Shaping Gut Microbiota in Pigs

NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells

Jersey steer ruminal papillae histology and nutrigenomics with diet changes

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