The effect of short-term treatment with vitamin D metabolites on bone lipid and mineral composition in healing vitamin D-deficient rats

Boskey, Adele L.; Carlo, Edward F. Di; Gilder, Helena; Donnelly, Robert; Weintroub, S.

doi:10.1016/8756-3282(88)90015-4

Cited by 16 publications

(5 citation statements)

References 30 publications

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“…The increased labeling from unmineralized to mineralized areas of cartilage and bone agrees with previously reported biochemical findings, indicating a higher phospholipid content from epiphyseal growth plate to metaphyseal bone (Wuthier 1982;Boskey et al 1988). Moreover, dentine, and mainly its mineralization front, appears to be more rich in phospholipids with respect to predentine (Goldberg et al 1995).…”

Section: Discussionsupporting

confidence: 91%

“…A possible role of phospholipids in the initial phases of mineral deposition (Shapiro et al 1966;Irving and Wuthier 1968;Prout et al 1973;Boskey et al 1988) is further suggested by these ultrastructural cytochemical observations, which can be considered as a useful method to further investigate the mechanism of the mineralization process and of its pathological alterations.…”

Section: Discussionmentioning

confidence: 98%

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Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Zini¹,

Sabatelli²,

Silvestrini

et al. 1996

Histochem Cell Biol

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The role of phospholipids in biological mineralization has been hypothesized but not fully elucidated. In order to identify phospholipids at the ultrastructural level in the mineralizing extracellular matrix, rat epiphyseal cartilage and metaphyseal bone have been labeled with the phospholipase A(2) (PLA(2))-gold method. The specificity and the efficiency of phospholipid detection have been evaluated by postembedding labeling of sections from epoxy- or hydrophilic resin-embedded samples, and by preembedding labeling of cryosectioned samples. The efficiency of the labeling was higher in cryosections than in hydrophilic resin-embedded specimens, while lower efficiency was found in epoxy resin-embedded samples. A 2- to 6-fold increase of the labeling density in calcified with respect to uncalcified areas of cartilage and bone has been found, depending on the specimen preparation used. The labeling intensity was significantly higher, at the periphery of the calcifying nodules in the epiphyseal cartilage matrix and in the calcifying osteoid, while the fully calcified bone matrix presented a weak labeling. Matrix vesicles, which are considered a possible source of extracellular phospholipids, appeared labeled in cryosections and in epoxy resin-embedded samples after a preincubation with PLA(2), which also increased the labeling of the intracellular membranes. The localization of phospholipids in the areas of initial mineralization suggests some hypotheses on the possible involvement of these molecules in the mineral phase deposition process.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone

Zini¹,

Sabatelli²,

Silvestrini

et al. 1996

Histochem Cell Biol

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“…Examples of the former include the vitamin-Ddeficient rat [119] and the second-generation vitamin-D-deficient rat, born to vitamin-D-deficient mothers [120], while the latter category includes the hypophosphatemic (HYP) mouse [53]. The animals in all of these models have negligible 1,25(OH) 2 D 3 and reduced 25 (OH)D 3 levels.…”

Section: Vitamin D Deficiencymentioning

confidence: 99%

“…The effect of first-vs. second-generation vitamin D deficiency is also inconsistent. In the second-generation vitamin-D-deficient rats, there are only small differences in bone ash weight, crystallinity, and Ca/P ratios when compared to age-matched controls [119]. These differences are greater in comparably aged, comparable gender and background, first-generation vitamin-D-deficient animals [120].…”

Section: Vitamin D Deficiencymentioning

confidence: 99%

Mineralization

Donnelly

Boskey

2011

Vitamin D

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“…An adequate intake of calcium and phosphorus in childhood is related to a lower incidence of osteoporosis in adulthood (21). Experimental animal models and data from human clinical practice have shown that deprivation of calcium, phosphorus, vitamin D and trace elements increase skeletal fragility (21)(22)(23)(24)(25)(26), although there is no indication that the present recommended dietary intake of calcium should be exceeded.…”

Section: Nutrition and Skeletal Mineralizationmentioning

confidence: 99%