The effect of silica‐containing calcium‐phosphate particles on human osteoblasts in vitro

Abstract

There is an ongoing need for more effective and less costly bone substitutes. It has previously been proposed that silica-containing bioactive glass would be more effective as a bone repair material because of its physiochemical properties. Three newly synthesized silica-containing bioactive glass formulations, HA-31 (25%), HA-11 (50%), and HA-13 (75%), were tested as biocompatible substrates for the continued proliferation and phenotype expression of human bone cells in vitro. Two currently available bioactive glasses (BioGlass(R), Hydroxyapatite) served as comparisons. The biocompatibility of these bioglasses, as well as their osteoconductive properties, was assessed by employing primary cultures of human osteoblasts and human synoviocytes for 4 days. The results obtained demonstrated that the three new bioglasses enhanced the proliferative response of osteoblasts compared with osteoblasts cultured alone. Reverse Transcription Polymerase Chain Reaction (RT-PCR) analysis indicated that osteoblasts retained their phenotypic expression by continued expression of collagen type I and alkaline phosphatase. The newly synthesized preparations of silica-containing bioactive glass did not induce stimulation of proinflammatory markers iNOS and IL-1beta in synoviocytes. In conclusion, the newly synthesized silica-containing bioactive glasses are biocompatible substrate for bone-forming osteoblasts. However, the formulations tested did not show significant advantage over the currently available bioactive glasses in vitro.

Mesoporous silica-calcium phosphate composites for experimental bone substitution

“…Silica containing calcium phosphate have shown osteoinductivity in human osteoblast cultures in vitro 15 and preparations with various silica and calcium phosphate compositions are under clinical studies 16 .…”

Development and Applications of Varieties of Bioactive Glass Compositions in Dental Surgery, Third Generation Tissue Engineering, Orthopaedic Surgery and as Drug Delivery System

“…In addition, pore distribution, interconnectivity and size are of paramount significance in order to assurance of proper cell proliferation and migration, as well as tissue vascularization and diffusion of nutrients. The concept of using bioactive glass substrates as templates for in vitro synthesis of bone tissue for transplantation by assessing the osteogenic potential has been investigated (Xynos et al, 2000;Phan et al, 2003;Chen et al, 2008;Brown et al, 2008) and confirmed that Bioglass scaffolds have potential as osteoconductive tissue engineering substrates for maintenance and normal functioning of bone tissue ). Human primary osteoblast-like cells cultured in contact with different bioactive glass suggested that bioglass not only induces osteogenic differentiation of human primary osteoblast-like cells, but can also increase collagen synthesis and release.…”

The examination of aerogel composite artificial bone substitutes in animal models

“…4,[6][7][8] Emellett a kalcium-foszfát tartalmú szilikagél humán in vitro osteoblast sejtkultúrákon mutatott oszteoinduktivitást. 5,9 Ezeken felül olyan tulajdonságaik is szerephez juthatnak, melyeket eddig még nem sikerült teljesen kiaknázni. A vizsgált minták elő-állítási technológiája lehetővé teszi az anyagban lévő pórusok méretének, ezzel együtt az átjárhatóságnak széles tartományban történő beállítását.…”

“…A jelölésre alkalmazott, kristályrácsba beépített európium-foszfátot nanokristályos formában az irodalomban már használták sejtek nyomjelzésére, nem volt toxikus, így nem vártunk különösebb reakciót. [9][10][11] A primer kísérleti nyomószilárd-sága 118 MPa, porozitása nagyobb, mint a későbbiekben említendő GY3-é.…”