The Effect of Silica (SiO2) to the Severity of Yellow Leaf Curl Disease on Chili Pepper

Selangga, ‪Dewa Gede Wiryangga; Hidayat, Sri Hendrastuti; Susila, Anas D.; Wiyono, Suryo

doi:10.22146/jpti.38951

Cited by 13 publications

(12 citation statements)

References 13 publications

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“…The DNA fragments in the two islands are similar to the Begomovirus DNA in chili plants in Yogyakarta and Central Java (580 bp) (Kandito et al 2019). The size of DNA fragments from chili Begomovirus PCR results in several areas in Indonesia has been previously reported, such as in Java and Sumatra, at 400 bp and in Bali at 912 bp (Fadhila et al 2022;Selangga et al 2019). Several countries have also previously reported the size of DNA fragments due to molecular identification of Begomovirus in chili plants by PCR.…”

Section: Discussionsupporting

confidence: 56%

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

WIDODO

TAUFIK²,

KHAERUNI³

et al. 2023

Biodiversitas

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Abstract. Widodo CJ, Taufik M, Khaeruni A, Mallaranggeng R. 2023. Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia. Biodiversitas 24: 741-751. Begomovirus disease is one of the main factors inhibiting chili cultivation which could harm plants and result in crop failure. This study aimed to determine the symptoms of begomovirus disease in chili plants in Buton and Muna, Southeast Sulawesi. The methods used are disease incidence survey, insect vector observation, molecular identification using the polymerase chain reaction (PCR) technique, and Sanger sequencing (Applied Biosystem 3500). The survey was conducted in Southeast Sulawesi province, covering Bau-Bau City, Buton Regency, Central Buton, North Buton, Muna, and West Muna. The results showed that the incidence and severity of Begomovirus disease were observed highest in Buton islands, 73% and 49.5% (Buton City), followed by North Buton, Central Buton, and Buton, with the incidence and severity of Begomovirus, respectively, of 71.74% and 59.5%; 69.32% and 51.5%; and 68% and 61.5%. While in Muna Regency, 63.16% and 38.5%, West Muna, 73.79% and 53.50%. On the other hand, the highest percentage of the whitefly population was in Bau-Bau City, 24%, while the lowest was in West Muna, 15.5% supporting this observation. Furthermore, PCR analysis successfully amplified symptomatic isolates from Buton and Muna islands at a band length of 580 bp. On the other hand, Sanger sequencing analysis revealed the existence of the Indonesian Pepper Yellow Leaf Curl Virus (PepYLCIV) with more than 96% genetic homology to the Gene Bank data of MN094866 PepYLCIV Capsicum annuum Indonesia and MN738463 PepYLCIV Chili pepper Indonesian. This information is the first report of the presence of Begomovirus on the two islands.

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Section: Discussionsupporting

confidence: 56%

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

WIDODO

TAUFIK²,

KHAERUNI³

et al. 2023

Biodiversitas

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“…Begomovirus has been reported in Indonesia in tomato, eggplant, cucumber, pumpkin and sweet potato . In Indonesia, Begomovirus infection in chili pepper was first reported in Java and several years later Selangga et al (2019), Selangga and Listihani (2021), reported that chili pepper crops in several regions in (Bali Province) had been infected by Begomovirus. The main type of Begomovirus in chili pepper is Pepper yellow leaf curl virus (PYLCV).…”

Section: Introductionmentioning

confidence: 99%

“…Natural transmission of Begomovirus occurs through insect vector B. tabaci in persistent circulative nature (Czosnek et al 2017). The study of Begomovirus transmission showed that the most efficient transmission occurs with acquisition feeding period for 24 hours and inoculation feeding period of 24 hours (Pan et al 2018;Selangga et al 2019). According to Shalev et al (2016) one adult B. tabaci vector after sucking inoculum source for 24 hours and going through inoculation period for 48 hours, can transmit the disease with an incidence rate of 30-50%.…”

Section: Introductionmentioning

confidence: 99%

Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

Temaja

SELANGGA²,

PHABIOLA³

et al. 2022

Biodiversitas

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Abstract. Temaja IGRM, Selangga DGW, Phabiola TA, Khalimi K, Listihani L. 2022. Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper. Biodiversitas 23: 5360-5366. Bemisia tabaci Genn is an important pest in horticulture crops. Until today, B. tabaci has always been found in chili pepper and causes damage. In addition to a direct injury to the chili pepper, it is also the vector of Begomovirus. There is no research till date regarding the relationship between B. tabaci with Begomovirus which caused damage to chili pepper crops. Thus, the aim of this research was to analyze the interaction between B. tabaci population with the disease incidence of Begomovirus on chili peppers crops, as well as the percentage of viruliferous B. tabaci. This research noted B. tabaci population dynamics, disease incidence of pepper yellow leaf curl disease, and the percentage of viruliferous B. tabaci via PCR by using Begomovirus universal primer and DNA analysis confirmation. The highest imago population occurred at 6 WAP with the average density 27.21 imago/yellow sticky card, while the highest nymph population occurred at 8 WAP with the average density of 4.26 nymph/leaf. The high population of B. tabaci nymph and imago caused the incidence of Begomovirus disease to reach 83.07% at 12 WAP. Result showed that high population of B. tabaci accelerated the speed of virus in the field. The confirmation result of Begomovirus in B. tabaci body showed that the virus was Pepper yellow leaf curl Indonesia virus (PYLCIV). The highest homology obtained was between PYLCIV isolates from Sekaan, Bangli with isolates from Bayung Gede, Bangli which ranged from 99.6 to 99.8%. Further phylogenetic analysis showed that PYLCIV isolates from Sekaan, Bali formed a group with the isolate from Bayung Gede, Bangli, which was from a different group with other isolates. This proved that B. tabaci carries and transmits PYLCIV to chili peppers in Bali.

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“…Green mosaic on the top of leaf was the early symptom chili pepper infecting viruses, followed by cupping upward or cupping downward and yellowing, plants will become dwarfs when infected with the virus as the seeds form. These types of symptoms had also been reported as typical of yellow leaf curl disease of chili pepper in Bali Island, Indonesia (Selangga et al, 2019). The type of symptoms associated with the disease in Bali Island is same as those from West Sumatra and Java, which yellowing, mosaic, curling, cupping and stunting were commonly found in West Sumatera (Trisno et al, 2009) and bright yellow colouring was observed in Java (Sulandari, 2004).…”

Section: Resultsmentioning

confidence: 72%

MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND

Selangga

Listihani

2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Molecular identification of Pepper yellow leaf curl Indonesia virus on chili pepper in Nusa Penida Island. Pepper yellow leaf curl Indonesia virus (PYLCV) has been reported as caused yellow leaf curl disease in Bali Island since early 2012. Dominant symptoms of PYLCV infection in chili pepper were yellowing, leaf curl, yellow mosaic, and mottle. Bemisia tabaci, has been known to vector on the case yellow leaf curl disease. Observations on the Nusa Penida Island in 2020 showed symptoms such as yellow leaf curl disease, however, identification of PYLCV in Nusa Penida Island has not been studied. Molecular identification was conducted using polymerase chain reaction and sequence analysis. Data collected in this study was disease symptoms and disease incidence. The results showed that dominant disease symptoms caused by virus from Nusa Penida were yellow mosaic, yellowing, and mottle. Universal DNA fragments of 912 bp were successfully amplified from 50 leaf samples using Begomovirus degenerate primers SPG 1 (5’-CCCCKGTGCGWRAATCCAT-3’) and SPG 2 (5’ATCCVAA YWTYCAGGGAGCT-3’). Sequence analysis showed that the isolate from Nusa Penida was a Pepper yellow leaf curl Indonesia virus with a 98–100% homology with several reference isolates.

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The Effect of Silica (SiO2) to the Severity of Yellow Leaf Curl Disease on Chili Pepper

Cited by 13 publications

References 13 publications

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia

Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND

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