The effect of simulated gastro-intestinal conditions on the antioxidant activity of herbal preparations made from native Irish hawthorn

Keating, Laura C.; Hayes, Jeremiah; Moane, Siobhán; Lehane, Mary; O’Doherty, Sinead; Kingston, Rosari; Furey, Ambrose

doi:10.1016/j.hermed.2014.05.003

Cited by 13 publications

(10 citation statements)

References 23 publications

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“…B. pilosa and D. cinerea hexane extracts lost their antibacterial activity following the treatment of SGF. Vermaak et al [51] and Keating et al [52] reported that SGF can modify or degrade antibacterial compounds in plants extracts and this can subsequently lead to the loss of initial antibacterial activity in the extracts. In the present study, loss of activity was observed with polar extracts of D. cinerea against S. typhimurium as compared with the plant extracts alone (Table 3) .…”

Section: Resultsmentioning

confidence: 99%

Antibacterial activity and in situ efficacy of Bidens pilosa Linn and Dichrostachys cinerea Wight et Arn extracts against common diarrhoea-causing waterborne bacteria

Shandukani

Tshidino

Masoko

et al. 2018

BMC Complement Altern Med

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BackgroundBidens pilosa and Dichrostachys cinerea extracts were investigated for the antibacterial properties against waterborne diarrhoeagenic bacteria.MethodsThe plant materials were extracted using the direct and serial exhaustive methods using solvents of varying polarities, namely, hexane, dichloromethane, ethyl acetate, acetone and methanol. Qualitative phytochemical analysis and quantitative determination of total phenolic content of the leaf powders of the two plants were tested. The antioxidant activities of the plants were determined using the 2, 2-diphenyl-1-picrylhydrazyl method. The toxic effect of the extracts on C2C12 muscle cell line were assessed by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method and the antibacterial activity was determined using the serial microbroth dilution.ResultsMethanol leaf extracts both plants had the highest yield in both direct and serial exhaustive extraction methods. Phytochemical profiling of the extracts displayed the presence of various secondary metabolites. The Benzene: ethanol: ammonia hydroxide solvent system showed a good resolution of chemical compounds in plant extracts from both plants. Most antioxidant compounds observed were developed in chloroform: ethyl acetate: formic acid and ethyl acetate: methanol: water solvent systems. All the bacterial species tested were sensitive to the effect of different extracts of both plant species, with E. coli being less sensitive to the effect of the extracts from D. cinerea. Following the simulated gastric fluid (SGF) treatment, a decrease in the antibacterial potency of the extracts was observed. No extract was toxic to the C2C12 muscle cell line.ConclusionThe presence of the secondary metabolites and nontoxic effect of the two plants tested may affirm the medicinal value of these leaf extracts. Our results suggest that B. pilosa and D. cinerea contain constituents with antioxidant and antimicrobial activities, which could be used in the treatment of diarrhoea in a case where untreated surface water is used.

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Section: Resultsmentioning

confidence: 99%

Antibacterial activity and in situ efficacy of Bidens pilosa Linn and Dichrostachys cinerea Wight et Arn extracts against common diarrhoea-causing waterborne bacteria

Shandukani

Tshidino

Masoko

et al. 2018

BMC Complement Altern Med

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“…On the other hand, herbal medicine is frequently used in subliminal clinical treatment [28,29]. In digestive system, ginger is reported to have a good facilitatory effects on the digestive problems in human [8].…”

Section: Discussionmentioning

confidence: 99%

Ginger-induced Ileal Motility is Modified by Stress: Sex Differences in Rats

Kimoto¹,

Zeredo²,

Ota³

et al. 2015

JFNS

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Abstract:Ginger is widely used as a spice and also an effective herbal medicine to treat gastrointestinal disorders. On the other hand, stress can induce various modulation of digestive motility. Here, we investigated ginger effects on stress-induced motility of the isolated ileum in male and female rats, in vitro. Rats (Wistar, SPF, 7-12 weeks of age, 148-393 g BW) were divided into Control (1G) and 3G groups. 3G stress (every day for 10 min) was loaded by centrifugal apparatus for 1, 3, 15 and 30 days. After the stress loading at each day, a 1 cm-long section of the ileum was isolated under barbiturate anesthesia and fixed to a Magnus-type chamber filled with Tyrode solution. Ileal movements were observed for 60 s following application of zingerone, which is also called vanillylacetone and a key component of the pungency of ginger (0.1-10 µM). Spontaneous motility movements with phasic and tonic patterns were observed in the ileum. The former was a peristalsis-like movement and the latter was a slow fluctuation of the baseline. Ginger induced enhanced effects on the rhythmic phasic motility in relation to amplitude. In the female, 3G gravity loading had no effects on the ginger-induced motility, however, suppressive effect of the ginger-induced phasic movements was clearly observed at day 15 in the male. The present study showed that gravity stress changed ginger-induced effects on phasic ileal motility in the male, but not in the female, indicating that sex differences were observed in the ginger effects modulated by stress loading.

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“…Herbal medicine is frequently used in subliminal clinical treatment in oriental countries [16,17]. Sansho is widely used in various herbal medicinal drugs as a principal or accessory component [18].…”

Section: Discussionmentioning

confidence: 99%

Sansho Intake Modulates Ileum Activity in Stress-loaded Rats

Kimoto¹,

Zeredo²,

Ota³

et al. 2015

JFNS

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Sansho (Japanese pepper) is a common spice widely used in Japanese cuisine. In addition, it is also an important component in Kampo medicine, such as in Daiken Chuto, which stimulates gastrointestinal motility and improves postoperative ileus. On the other hand, we previously reported that gravity stress loading produce negative effects on ileal movements. In the present study, we report changes in ileal motility after gravity-stress with and without Sansho intake in male and female rats. Ileal movements were activated by topical Acetylcholine (Ach) application, and maximum amplitudes (MA) of the evoked contraction were compared. Clear tonic patterns were observed in the ileal motility after Ach application. After short-term stress, there were no significant differences in MA between control and Sansho-intake groups in both sexes. However, after long-term stress-loading, significant enhancement of MA was observed in Sansho-intake group in males, but not in females. The present study showed that SAN affected Ach-induced ileal motility in the male, but not in the female after long-term stress loading, indicating sex differences in effects of Sansho intake. It is suggested that Sansho is more effective in males than in females to decrease negative stress response.

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The effect of simulated gastro-intestinal conditions on the antioxidant activity of herbal preparations made from native Irish hawthorn

Cited by 13 publications

References 23 publications

Antibacterial activity and in situ efficacy of Bidens pilosa Linn and Dichrostachys cinerea Wight et Arn extracts against common diarrhoea-causing waterborne bacteria

Antibacterial activity and in situ efficacy of Bidens pilosa Linn and Dichrostachys cinerea Wight et Arn extracts against common diarrhoea-causing waterborne bacteria

Ginger-induced Ileal Motility is Modified by Stress: Sex Differences in Rats

Sansho Intake Modulates Ileum Activity in Stress-loaded Rats

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