The effect of single and repeated UVB radiation on rabbit cornea

Abstract

For the first time, the effects of single and repeated UVR doses on the metabolic profile of the rabbit cornea were analysed and compared. The combination of HR-MAS 1H NMR spectroscopy and modern statistical methods (multivariate analysis, one-way ANOVA) proved suitable to assess the overall view of the metabolic alterations in the rabbit corneal tissue following UVB radiation exposure.

The effect of single and repeated UVB radiation on rabbit lens

“…Thus, at this wavelength, corneal tissue absorbs the main amount of the harmful UVR energy and is supposed to be the most vulnerable segment of the eye towards the effects of UV irradiation. These presumptions were confirmed by the PCA results, both in our previous experiments [12,14] and the present study. As apparent in Fig.…”

“…The resonances of other metabolites either overlapped each other or the signal to cornea -e, f) [12,14]. Score plot representation (a, c, e); loading profile of PC1 (b, d, f).…”

“…In our previous experiments [12,14], a substantial cumulative effect of UVR-B on the metabolic profiles of rabbit cornea and aqueous humour was revealed. By using identical experimental design, the purpose of the present study was to investigate alterations in the rabbit lens, obtained from the same eye as the recently examined corneal and aqueous humour samples, and thereby supplement and complete the findings of our prior investigations.…”

Favorable effects of trehalose on the development of UVB-mediated antioxidant/pro-oxidant imbalance in the corneal epithelium, proinflammatory cytokine and matrix metalloproteinase induction, and heat shock protein 70 expression

“…The cornea absorbs and detoxifies the majority of UVB rays reaching the eye, however, when a threshold amount of UVB rays is exceeded, a series of harmful disturbances appears, such as morphological damage of the corneal epithelium [2][3][4][5], alterations in corneal optics [6], changes in corneal metabolites [7][8][9], the induction of reactive oxygen and nitrogen species [10], and proinflammatory cytokine expression [11]. Additionally, an antioxidant/pro-oxidant imbalance appears in the corneal epithelium [12][13][14][15].…”

Postnatal Biochemical Changes in Rat Lens: An Important Factor in Cataract Models

“…In our laboratory, HR-MAS 1 H NMR spectroscopy has already demonstrated its suitability in similar experiments and was successfully applied in animal eye studies. [10][11][12][13]18,21,22,26 Especially the alterations in the concentrations of antioxidants (GSH), osmolytes (taurine, hypo-taurine, and myoinositol), and membrane constituents (phosphocholine) accompanying the formation of opacity in the cataractous rabbit and rat lenses were easily assessed with no need for prior multiextraction procedures. [10][11][12][13]18,21,22 In spite of a generally poorer sensitivity of the NMR techniques and use of relative quantification in the HR-MAS 1 H NMR spectroscopy, the direct use of intact tissue makes it superior to other methods.…”