The effect of sodium hypochlorite concentration on extraction of poly-β-hidroxy-butyrate (PHB) produced from soil bacteria Burkholderia sp B37

Abstract

Burkholderia sp., a soil bacterium, is a poly-hydroxy-butyrate (PHB) producer. A study reported that sodium hypochlorite solution (NaOCl) is very effective and immediate to lysis the cell wall of bacteria. However, no data available on the effect of NaOCl concentration on extraction of PHB produced from Burkholderia sp. B37. We therefore aimed to evaluate the effect of NaOCl concentrations (2.5, 5, and 12.5 % v/v) on the extractability of produced PHB from Burkholderia sp. B37. The PHB production was carried out in the Ramsay’s minimal medium. The PHB extracted were purified and identified by Gas-Chromatography-Mass Spectrometry (GC-MS) then further evaluated for its physicochemical characteristics including surface morphology, functional group properties by Fourier transform infra-red (FTIR) spectroscopy and thermal characteristic. The results showed that NaOCl facilitates the extraction of produced PHB by Burkholderia sp. B37. The concentration of NaOCl influenced on the extractability of PHB and the surface morphology of purified PHB obviously displayed cleaner surface area when 12.5 % v/v of NaOCl was applied. The FTIR and thermal analysis showed no change detected on the functional group properties and full degradation of purified PHB. However, more works are needed to optimize the yield of PHB recovered.