The Effect of Sperm Concentration in the Ejaculate on Morphological Traits of Bull Spermatozoa

Kondracki, Stanisław; Banaszewska, Dorota; Wysokńjska, Anna; Iwanina, Maria

doi:10.3409/fb60_1-2.85-91

Cited by 30 publications

(25 citation statements)

References 32 publications

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“…A very simple technique that has been used for many years to evaluate mammalian semen is staining with a complex of eosin and gentian stain (26,27). This method stains sperm heads very distinctly but makes observation of the boundary of the acrosome or the midpiece difficult, which was confirmed in the present study.…”

Section: Discussionsupporting

confidence: 71%

Morphometric dimensions of the stallion sperm head depending on the staining method used

Banaszewska¹,

Czubaszek

Walczak-Jędrzejowska

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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Semen was collected from Polish Halfbred stallions. Twenty individuals from 3 to 4 years of age were selected for the study. At least one ejaculate from each stallion was collected and assessed. Sperm morphology was evaluated using Papanicolaou stain, SpermBlue ® , and a complex of eosin and gentian stain, whereas unstained slides were used as control samples. Morphometric measurements were performed on 100 randomly selected sperm heads in each ejaculate. The length, width, perimeter, and surface area of the sperm head were measured. The frequency of nuclear vacuoles was determined as well. Tygerberg's strict criteria, which most precisely characterise the sperm head, were used in the morphological evaluation of the sperm. The results obtained indicate that in the case of staining with SpermBlue ® and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears. It also seems that neither shrinking nor swelling is uniform in the various staining techniques (Papanicolaou stain). Moreover, it appears that in comparison with unstained sperm, all methods caused the width of the head to increase as a result of swelling.

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Section: Discussionsupporting

confidence: 71%

Morphometric dimensions of the stallion sperm head depending on the staining method used

Banaszewska¹,

Czubaszek

Walczak-Jędrzejowska

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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“…Routine assessments of sperm morphology in cattle and pigs predominantly use the eosin-gentian or eosin-nigrosin staining proposed by Hancock (1951). Eosin is particularly recommended for quick evaluation of bull sperm (Chenoweth et al 1992, Chenoweth 2005, Enciso et al 2011, Kondracki et al 2006, 2012. Studies by Kondracki et al (2006) and Banaszewska et al (2011) confirm that eosin staining is the standard technique for assessment of sperm morphology in farm animals, commonly applied at animal breeding and insemination centres.…”

Section: Discussionmentioning

confidence: 99%

“…The smears were examined microscopically using immersion lenses with a 100x zoom on an Olympus BX50 light microscope. In each smear, morphometric measurements were performed on 30 randomly selected morphologically well-formed spermatozoa, well visible in the field of vision of the microscope, according to a method devised by Kondracki et al (2006Kondracki et al ( , 2012. A total of 600 spermatozoa were examined.…”

Section: Agno 3 Stainingmentioning

confidence: 99%

Comparison of different chromatin staining techniques for bull sperm

Andraszek¹,

Banaszewska

Czubaszek³

et al. 2014

Arch. Anim. Breed.

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Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In the fully developed, mature sperm, the histones are replaced with protamines. Disruptions of nucleoprotein structure can be treated as possible indicators of the biological value of spermatozoa. The experimental material consisted of sperm from one-and-a-half-year-old bulls, isolated post mortem from the tail of the epididymis. The smears were stained with silver nitrate (AgNO 3 ), acridine orange (AO), aniline blue (AB) and chromomycin A3 (CMA3). Sperm dimensions largely depend on individual variability among the bulls. In most cases, differences in sperm dimensions were identified between individuals, which was confirmed in the statistics. Sperm with elevated, abnormal histone levels were proportionally quite scarce (1.4 %). Studies of nuclear proteins in the context of infertility demonstrate the important influence of normal chromatin structure on sperm functions.

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“…The Papanicolaou method is used for analysis of human semen (Menkveld et al, 1990;WHO, 1999). For evaluation specifically of bull semen, a staining technique using a complex of eosin and gentian violet is recommended (Blom, 1981;Kondracki et al, 2012). As an experimental method, staining with silver nitrate, which enables more precise analysis of individual structures of the sperm cell, may be used as well (Andraszek and Smalec, 2011;Andraszek et al, 2014a,b).…”

Section: Q4mentioning

confidence: 99%

“…Silver nitrate is a stain with basic pH used to identify acidic chromatin proteins. However, for routine evaluation of sperm morphology in Poland, conducted at animal breeding and insemination centres, the most commonly used stain is the acidic eosin + gentian violet complex, which is recommended for bull semen (Blom, 1981;Kondracki et al, 2012).…”

Section: Morphometric Traitsmentioning

confidence: 99%