The effect of storage at low temperature on the haemolytic complement activity of chicken serum

Demey, F.; Pandey, V. S.; Baelmans, R.; Agbede, G.; Verhulst, A.

doi:10.1007/bf01839178

Cited by 21 publications

(13 citation statements)

References 6 publications

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“…Complement activity was determined with a hemolytic technique as described previously (Demey et al 1993, Parmentier et al 2002 using an adapted light-scattering method. Readings were transformed by log-log equation (Demey et al 1993, Parmentier et al 2002 and the hemolytic titer was expressed as the titer that lysed 50% of the erythrocytes (CH50 U/mL). Plates were shaken in a Titertek (Flow Laboratories, Titertek-Berthold, Pforzheim, Germany) every 30 min during the period of incubation.…”

Section: Humoral Immunitymentioning

confidence: 99%

“…Different techniques for the determination of humoral immunity molecules in the eaglets' blood, including complement (the classical complement pathway [CPW] route and alternative complement pathway [APW] route), interleukins (IL1b, IL8-like), and c interferon (IFN-c) were used. Complement activity was determined with a hemolytic technique as described previously (Demey et al 1993, Parmentier et al 2002 using an adapted light-scattering method. In brief, sera were diluted serially in appropriate buffers in flat-bottomed 96-well microtiter plates and incubated with sensitized (Haemolysin, reference number 72202; Biome´rieux España, Madrid, Spain) sheep erythrocytes to measure CPW or rabbit erythrocytes to measure APW.…”

Section: Humoral Immunitymentioning

confidence: 99%

“…The results (the amount of light scattering by erythrocytes upon lysis) were read at 655 nm in a microtiter reader (BioRad model 3550). Readings were transformed by log-log equation (Demey et al 1993, Parmentier et al 2002) and the hemolytic titer was expressed as the titer that lysed 50% of the erythrocytes (CH50 U/mL).…”

Section: Humoral Immunitymentioning

confidence: 99%

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When conservation management becomes contraindicated: impact of food supplementation on health of endangered wildlife

Blanco

Lemus

García-Montijano³

2011

Ecological Applications

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Understanding the conditions that force the implementation of management actions and their efficiency is crucial for conservation of endangered species. Wildlife managers are widely and increasingly using food supplementation for such species because the potentially immediate benefits may translate into rapid conservation improvements. Supplementary feeding can also pose risks eventually promoting undesired, unexpected, subtle, or indirect, and often unnoticed, effects that are generally poorly understood. For two decades, intensive food supplementation has been used in attempting to improve the breeding productivity of the Spanish Imperial Eagle, Aquila adalberti, one of the most endangered birds of prey in the world. Here, we examined the impact of this intensive management action on nestling health, including contamination, immunodepression, and acquisition of disease agents derived from supplementation techniques and provisioned food. Contrary to management expectations, we found that fed individuals were often inadvertently "medicated" with pharmaceuticals (antibiotics and antiparasitics) contained in supplementary food (domestic rabbits). Individuals fed with medicated rabbits showed a depressed immune system and a high prevalence and richness of pathogens compared with those with no or safe supplementary feeding using non-medicated wild rabbits. A higher presence of antibiotics (fluoroquinolones) was found in sick as opposed to healthy individuals among eaglets with supplementary feeding, which points directly toward a causal effect of these drugs in disease and other health impairments. This study represents a telling example of well-meaning management strategies not based on sound scientific evidence becoming a "contraindicated" action with detrimental repercussions undermining possible beneficial effects by increasing the impact of stochastic factors on extinction risk of endangered wildlife.

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Section: Humoral Immunitymentioning

confidence: 99%

Section: Humoral Immunitymentioning

confidence: 99%

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When conservation management becomes contraindicated: impact of food supplementation on health of endangered wildlife

Blanco

Lemus

García-Montijano³

2011

Ecological Applications

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“…Both classical calcium-dependent (CPW) and alternative calcium-independent (APW) complement activity were determined with a haemolytic technique (Demey et al, 1993;Parmentier et al, 2002) using an adapted light-scattering method. Activity was expressed as CH 50 U/ml, this being the difference between initial complement levels and the complement levels at given days post vaccination (dpv).…”

Section: Complementmentioning

confidence: 99%

Reciprocal Antibody and Complement Responses of Two Chicken Breeds to Vaccine Strains of Newcastle Disease Virus, Infectious Bursal Disease Virus and Infectious Bronchitis Virus

et al. 2006

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“…Complement activity was determined with a haemolytic technique as described elsewhere (Demey et al, 1993) using an adapted light-scattering method. Brie£y, sera were diluted serially in appropriate bu¡ers in £at-bottomed 96-well microtitre plates and incubated with sensitized sheep erythrocytes to measure CPW or rabbit erythro- NanaFfDw^Frizzled and naked neck 8 NanaFfdw^Frizzled, naked neck and dwarf cytes to measure APW.…”

Section: Complement Assaysmentioning

confidence: 99%

Serum Haemolytic Complement Levels in German Dahlem Red Chickens Are Affected by Three Major Genes (Naked Neck, Dwarf, Frizzled) of Tropical Interest

Dorny

Baelmans

Parmentier

et al. 2005

Tropical Animal Health and Production

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2005. Serum haemolytic complement levels in German Dahlem Red chickens are a¡ected by three major genes (naked neck, dwarf, frizzled) of tropical interest. Tropical Animal Health and Production, 37(1), 1^9 ABSTRACT German Dahlem Red chickens with three di¡erent major genes of tropical interest: Nana^(naked neck), Ff^(frizzled) and dw^(dwarf), respectively, were tested for serum haemolytic complement, which is essential in innate host defence against infectious agents. Eight di¡erent combinations of genes for body size and feather coverage were evaluated. Signi¢cant di¡erences both for both the calcium-dependent (classical, CPW) and the calcium-independent (alternative, APW) complement titres were found between the phenotypes. Phenotype nana¡Dw^showed the highest complement status. The frizzled (Ff^) gene had a negative in£uence on APW titres, whereas the dwarf (dw^) gene had a negative in£uence on CPW titres. The naked neck (Nana^) gene had various in£uences on the haemolytic complement status. All tested hens had MHC (B) 21 haplotypes, whereas the gene for dwar¢sm appeared to be linked with the B19 haplotype. It was concluded that introducing major genes (Nana^, dw^, Ff^) to conquer environmental stress in hot climates can have a negative impact on certain aspects of the innate immunity of poultry.

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The effect of storage at low temperature on the haemolytic complement activity of chicken serum

Cited by 21 publications

References 6 publications

When conservation management becomes contraindicated: impact of food supplementation on health of endangered wildlife

When conservation management becomes contraindicated: impact of food supplementation on health of endangered wildlife

Reciprocal Antibody and Complement Responses of Two Chicken Breeds to Vaccine Strains of Newcastle Disease Virus, Infectious Bursal Disease Virus and Infectious Bronchitis Virus

Serum Haemolytic Complement Levels in German Dahlem Red Chickens Are Affected by Three Major Genes (Naked Neck, Dwarf, Frizzled) of Tropical Interest

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