The effect of strontium-substituted hydroxyapatite coating on implant fixation in ovariectomized rats

Li, Yunfeng; Li, Qing; Zhu, Songsong; Luo, En; Li, Jihua; Feng, Ge; Liao, Yunmao; Hu, Jing

doi:10.1016/j.biomaterials.2010.07.112

Cited by 265 publications

(196 citation statements)

References 43 publications

(46 reference statements)

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“…Several methods have been tested for the deposition of CaP coating on Ti implants, including PS, sputtering, sol-gel deposition, and electrophoretic deposition processes, but PS is considered the most successful so far [28]. Strontium-containing HA coatings, grown by PLD or sol gel methods, have also been proposed in order to improve the osseointegration process [39,40].…”

Section: Inorganic Functional Coatingsmentioning

confidence: 99%

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

et al. 2016

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Surface modification of dental implants is a key process in the production of these medical devices, and especially titanium implants used in the dental practice are commonly subjected to surface modification processes before their clinical use. A wide range of treatments, such as sand blasting, acid etching, plasma etching, plasma spray deposition, sputtering deposition and cathodic arc deposition, have been studied over the years in order to improve the performance of dental implants. Improving or accelerating the osseointegration process is usually the main goal of these surface processes, but the improvement of biocompatibility and the prevention of bacterial adhesion are also of considerable importance. In this review, we report on the research of the recent years in the field of surface treatments and coatings deposition for the improvement of dental implants performance, with a main focus on the osseointegration acceleration, the reduction of bacterial adhesion and the improvement of biocompatibility.

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Section: Inorganic Functional Coatingsmentioning

confidence: 99%

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

et al. 2016

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“…Our results demonstrated that a low concentration of SrCl 2 (5 lg/mL) improved BMSCs osteogenic differentiation, accordingly with data by Sila-Asna et al 24 Strontium has a positive effect on bone metabolism 21 and on MSCs osteogenic differentiation. 26,28 Furthermore, it has been demonstrated to improve osseointegration when delivered at the implant site by direct incorporation within titanium, 19 or inclusion in a hydroxyapatite coating 20 and in bone graft extenders. 46 As the in vivo effect of strontium on bone metabolism is associated with increased BMSCs osteogenesis, 27 we believe that the simultaneous inclusion of BMSCs and strontium in a hydrogel loaded into titanium may promote bone ingrowth by a dual action on host progenitor cells resident at the implant site and on cells encapsulated into the hydrogel.…”

Section: Selection Of Srcl 2 Concentration To Improve Bmscs Osteogenimentioning

confidence: 99%

“…Strontium is a low-cost ion and is easier to handle compared to osteoinductive proteins, such as BMP-2, being therefore an ideal candidate to enrich off-the-shelf materials for bone tissue engineering, as proposed for hydroxyapatite 17,18 and, more recently, for titanium. 19,20 Indeed, strontium ranelate is a drug used for the treatment of osteoporosis, thanks to its ability to increase bone neoformation and to reduce bone resorption. [21][22][23] Furthermore, strontium has a positive effect on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), [24][25][26][27][28] which are resident at the implant site and are characterized by an intrinsic osteogenic potential.…”

Section: Introductionmentioning

confidence: 99%

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Lopa¹,

Mercuri

Colombini³

et al. 2013

J Biomedical Materials Res

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Insufficient implant stability is an important determinant in the failure of cementless prostheses. To improve osseointegration, we aim at generating a bioactive implant combining a macroporous titanium (TT) with a biocompatible hydrogel to encapsulate osteo-inductive factors and osteoprogenitor cells. Amidation and cross-linking degree of an amidated carboxymethylcellulose hydrogel (CMCA) were characterized by FT-IR spectrometry and mechanical testing. Bone marrow mesenchymal stem cells (BMSCs) from osteoarthritic patients were cultured on CMCA hydrogels, TT, and TT loaded with CMCA (TT þ CMCA) with an optimized concentration of SrCl 2 to evaluate cell viability and osteo-differentiation. Amidation and cross-linking degree were homogeneous among independent CMCA batches. SrCl 2 at 5 lg/mL significantly improved BMSCs osteo-differentiation increasing calcified matrix (P < 0.01), type I collagen expression (P < 0.05) and alkaline phosphatase activity. TT þ CMCA samples better retained cells into the TT mesh, significantly improving cell seeding efficiency with respect to TT (P < 0.05). BMSCs on TT þ CMCA underwent a more efficient osteo-differentiation with higher alkaline phosphatase (P < 0.05) and calcium levels compared to cells on TT. Based on these in vitro results, we envision the association of TT with strontium-enriched CMCA and BMSCs as a promising strategy to generate bioactive implants promoting bone neoformation at the implant site.

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“…14,18,19,23 Studies on the chemical composition of nanotopographies have highlighted the significance of HA in promoting cell response, 29,30 and strontium-doped HA (Ca 10-x Sr x [PO 4 ] 6 [OH] 2 , Sr x -HA) with a Sr dose lower than 20% enhances osteoblast activity 31 and the positive effect of HA on bone integration. 32 In our previous works, we prepared nanogranulated HA, 33 Sr 0.5 -HA-, Sr 1 -HA-, Sr 2 -HA-, 32 and Sr 10 -HA- 34 patterned TiO 2 coatings using microarc oxidation (MAO), and also synthesized nanorod-shaped Sr 1 -HA-patterned TiO 2 coatings on macroporous titanium scaffolds 35 and dense titanium 36 using a two-step method of MAO and hydrothermal treatment (HT). It has been revealed that these multilayer coatings can firmly adhere to titanium substrates, 35 and the proliferation and differentiation of osteoblasts can be positively or negatively regulated by the narrow or wide interrod spacing of the Sr 1 -HA nanorods in the presence of serum, respectively.…”

Section: Introductionmentioning

confidence: 99%

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

Zhou¹,

Han²,

Lu³

2014

IJN

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The process in which nanostructured surfaces mediate cell adhesion is not well understood, and may be indirect (via protein adsorption) or direct. We prepared Sr-doped hydroxyapatite (Sr 1 -HA) 3D nanorods (with interrod spacing of 67.3±3.8, 95.7±4.2, and 136.8±8.7 nm) and 2D nanogranulate patterned coatings on titanium. Employing the coatings under the same surface chemistry and roughness, we investigated the indirect/direct role of Sr 1 -HA nanotopographies in the regulation of osteoblast adhesion in both serum-free and serum-containing Dulbecco’s Modified Eagle/Ham’s Medium. The results reveal that the number of adherent cells, cell-secreted anchoring proteins (fibronectin, vitronectin, and collagen), vinculin and focal adhesion kinase (FAK) denoted focal adhesion (FA) contact, and gene expression of vinculin, FAK, and integrin subunits (α 2 , α 5 , α v , β 1 , and β 3 ), undergo significant changes in the inter-nanorod spacing and dimensionality of Sr 1 -HA nanotopographies in the absence of serum; they are significantly enhanced on the <96 nm spaced nanorods and more pronounced with decreasing interrod spacing. However, they are inhibited on the >96 nm spaced nanorods compared to nanogranulated 2D topography. Although the adsorption of fibronectin and vitronectin from serum are higher on 136.8±8.7 nm spaced nanorod patterned topography than nanogranulated topography, cell adhesion is inhibited on the former compared to the latter in the presence of serum, further suggesting that reduced cell adhesion is independent of protein adsorption. It is clearly indicated that 3D nanotopography can directly modulate cell adhesion by regulating integrins, which subsequently mediate anchoring proteins’ secretion and FA formation rather than via protein adsorption.

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The effect of strontium-substituted hydroxyapatite coating on implant fixation in ovariectomized rats

Cited by 265 publications

References 43 publications

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings

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