The Effect of Subinhibitory Concentrations of Clindamycin on the Adherence and Glycocalyx of Staphylococcus Aureus and Bacteroides Species in Vitro and in Vivo

LambeJr., Dwight W.; Mayberry-Carson, K J; Mayberry, W. R.; Tober-Meyer, B; Costerton, J. W.

doi:10.1007/978-1-4613-1901-6_4

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…Both antimicrobial agents at concentrations of less than 2 g/ml significantly reduced S. epidermidis glycocalyx formation and adherence to vascular catheters (14). As reported by Lamb et al (16), subinhibitory concentrations of clindamycin inhibit the adherence of Bacteroides species and E. coli to bone surfaces in vitro.…”

Section: Vol 39 1995 Interaction Between Fluoroquinolones and Biofisupporting

confidence: 58%

“…Extracellular slime or glycocalyx production is an important factor in the adherence of bacteria and their protection from host defense mechanisms. Extensive glycocalyx production has been demonstrated on cultures of Staphylococcus epidermidis (14), S. aureus (4), Pseudomonas aeruginosa (15), Escherichia coli (3), and Bacteroides species (16).…”

mentioning

confidence: 99%

“…The combination of tobramycin and piperacillin (1), the combination of tosufloxacin and erythromycin (12), clindamycin (16), and clarithromycin (27,28) have been reported to be effective in eradication of biofilm-associated bacteria. However, the clinical usefulness of these experimentally effective strategies remains to be established (27).…”

mentioning

confidence: 99%

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Modulation of biofilms of Pseudomonas aeruginosa by quinolones

Yassien

Khardori

Ahmedy

et al. 1995

Antimicrob Agents Chemother

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The interaction between four fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and ofloxacin) and biofilms of Pseudomonas aeruginosa in wells of microtiter plates and on segments of vascular catheters was studied in an in vitro model of vascular catheter colonization. Subinhibitory concentrations (one-half, onefourth, and one-eighth of the MIC) of the fluoroquinolones reduced the adherence of P. aeruginosa to 30 to 33, 44 to 47, and 61 to 67% of that of controls, respectively. The addition of high concentrations of the fluoroquinolones (12.5 and 400 g/ml) to preformed biofilms (grown for 48 h at 37؇C) decreased the adherence of P. aeruginosa to 69 to 77 and 39 to 60% of that of controls, respectively. In an in vitro model of vascular catheter colonization, subinhibitory concentrations (one-half, one-fourth, and one-eighth of the MIC) of fluoroquinolones reduced the number of adherent bacteria to 21 to 23, 40 to 46, and 55 to 70% of that of the controls, respectively. Scanning electron microscopy demonstrated a significant reduction in glycocalyx formation and adherent bacteria in the presence of pefloxacin at one-half to one-eighth of the MIC. Vascular catheter segments precolonized with P. aeruginosa for 24 h and exposed to the fluoroquinolones at 4 to 25 times the MIC (50 g/ml) for 2 h showed <5% growth of adherent cells compared with controls. No adherent organisms were cultured in the presence of 8 to 50 times the MIC (100 g/ml). Scanning electron microscopy studies of preformed biofilms exposed to pefloxacin verified the results obtained by culture. These data show that subinhibitory concentrations of ciprofloxacin, norfloxacin, pefloxacin, and ofloxacin inhibit the adherence of P. aeruginosa to plastic surfaces and vascular catheters. Clinically achievable concentrations of fluoroquinolones (50 to 100 g/ml) were able to eradicate preformed biofilms on vascular catheters.The use of various medical devices such as indwelling vascular catheters, cardiac pacemakers, prosthetic heart valves, chronic ambulatory peritoneal dialysis catheters, and prosthetic joints has greatly facilitated the medical and surgical management of serious illnesses. Bacterial infections following colonization and biofilm formation on these devices are a major cause of morbidity in patients receiving prosthetic devices (5). Biofilms have also been demonstrated on tissues in chronic bacterial diseases that are characterized by resistance to antimicrobial chemotherapy (27). Extracellular slime or glycocalyx production is an important factor in the adherence of bacteria and their protection from host defense mechanisms. Extensive glycocalyx production has been demonstrated on cultures of Staphylococcus epidermidis (14), S. aureus (4), Pseudomonas aeruginosa (15), Escherichia coli (3), and Bacteroides species (16).Different approaches (including the use of various antimicrobial agents) to the eradication of biofilm bacteria have been studied. The combination of tobramycin and piperacillin (1), the combination of tosufloxacin and er...

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Section: Vol 39 1995 Interaction Between Fluoroquinolones and Biofisupporting

confidence: 58%

mentioning

confidence: 99%

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Modulation of biofilms of Pseudomonas aeruginosa by quinolones

Yassien

Khardori

Ahmedy

et al. 1995

Antimicrob Agents Chemother

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“…Dicloxacillin is the antibiotic that found to prevent the adherence to the greatest extent when it is used alone at 1/2 of the MIC (Cerca et al, 2005). Also clindamycin at subinhibitory concentrations inhibits the adherence of Pseudomonas aeruginosa, Staphylococcus aureus, Bacteroids spp., Escherichia coli to bone surfaces (Lambe et al, 1987).…”

Section: A Antimicrobial Agentsmentioning

confidence: 99%

Application of Scanning Electron Microscopy for the Morphological Study of Biofilm in Medical Devices

El-Baky¹

2012

Scanning Electron Microscopy

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“…This is in keeping with the spectrum of bacteria responsible for peritonitis in CAPD patients [15,16]. Also, the production of biofilms by these bacterial populations in different human infections is well documented in the literature [1,9,11,12,[17][18][19][20], These studies [13,14] were made on TC that had been removed. Extensive BB colon ization developed over time on both the extra-and intraluminal surfaces of the TC in both control and study groups and, surpris ingly, the extent of BB growth on TC re moved from different groups of patients, with and without peritonitis, was similar.…”

Section: Demonstration O F Bb In Tc Removed From Capd Patientsmentioning

confidence: 99%

Significance of Biofilm-Adherent Bacterial Microcolonies on Tenckhoff Catheters of CAPD Patients

Dasgupta

Costerton

1989

Blood Purif

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The phenomenon of biofilm bacterial adherence to bioprosthetic devices and their relationship to various human infections are now well established. We have recently demonstrated that biofilm bacterial colonization of Tenckhoff catheters (TC) is very common in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) treatment. Based on our current work related to isolation of biofilm bacteria from peritoneal effluents of CAPD patients, we describe a novel way of diagnosis of this form of infection by using a modified Robbins device. Additionally, we describe the possible interaction of host defense factors with biofilm bacteria and ways to eliminate their colonization of TC for developing an overall strategy to prevent CAPD-associated peritonitis.

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The Effect of Subinhibitory Concentrations of Clindamycin on the Adherence and Glycocalyx of Staphylococcus Aureus and Bacteroides Species in Vitro and in Vivo

Cited by 10 publications

References 18 publications

Modulation of biofilms of Pseudomonas aeruginosa by quinolones

Modulation of biofilms of Pseudomonas aeruginosa by quinolones

Application of Scanning Electron Microscopy for the Morphological Study of Biofilm in Medical Devices

Significance of Biofilm-Adherent Bacterial Microcolonies on Tenckhoff Catheters of CAPD Patients

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