Application of Scanning Electron Microscopy for the Morphological Study of Biofilm in Medical Devices

El-Baky, Rehab Mahmoud Abd

doi:10.5772/35261

Cited by 5 publications

(5 citation statements)

References 64 publications

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“…The strains showed less adhesion to PVC as compared to silicone and latex. These results are in accordance with other studies performed by many laboratories where it has been reported that microbial adherence to biomaterials occurs in the following order: latex > silicone > PVC > Teflon > Polyurethane > stainless steel > titanium (Abd El-Baky, 2012). Dwornicz et al (2003) found that Enteroccocus feacalis adheres highly to silicone and siliconized latex than to PVC.…”

Section: Discussionsupporting

confidence: 92%

“…The widespread use of medical devices has caused a great advance in the management of many diseases. Indwelling medical devices are being increasingly used for the treatment of functional deficits in numerous medical fields (Abd El-Baky, 2012). Despite the considerable success achieved with new material devices, these abiotic surfaces are susceptible to bacterial colonization which creates an important public health problem (Treter and Macedo, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Imane¹,

Hassaïne²,

Bellifa³

et al. 2014

Afr. J. Microbiol. Res.

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Acinetobacter baumannii is an opportunistic pathogen responsible for nosocomial infections due to biofilm formation on the surface of implantable medical devices. Thirty (30) strains of A. baumannii were isolated from medical devices and tested for their ability to form a biofilm. The factors that may influence this process, such as the hydrophobicity of the bacterial wall, temperature, duration of implantation and the nature of the medical device, were also investigated. Strains were able to form a biofilm; however this process was more substantial at 30°C than at 37°C and was maximal after 96 h of incubation. Strains seem to adhere better to silicone and latex than to polyvinylchloride (PVC) and no apparent relationship was found between hydrophobicity and biofilm formation.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Imane¹,

Hassaïne²,

Bellifa³

et al. 2014

Afr. J. Microbiol. Res.

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“…The bacterial cocci appeared elongated, swollen, scattered and change in their orientations .These changes were due to the pharmacological effects of fluoride. These results came with the same results reached by Abd El-Baky R. M (2012) who treated S. aureus with ciprofloxacin-N-acetylcystein [14]. As it was observed using SEM, dramatic decline in formed biofilms of the three tested strains was observed on the four tested surfaces after the application of tooth paste contained 0.45% for 30 min (Figure 2 - Figure 5).…”

Section: Discussionsupporting

confidence: 88%

“…D-Each tested surfaces was washed with phosphate buffer saline( PBS) ,fixed in 2% glutteraldehyde for 1h at 37°C then washed again with PBS twice times and left to dry at room temperature [14].…”

Section: Biofilm Formation Proceduresmentioning

confidence: 99%

Antibiofilm of a Tooth Paste on Cariogenic Bacteria

Aladool¹,

Abdul-Rahman²

2018

IJDSR

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Caries is an infectious diseases of the oral cavity in which oral biofilms play a causative role. Moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and drugs acting on biofilm to, due to their widespread presence and accessibility. The aim of the present in vitro study was to investigate the efficacy of specific fluoridated toothpaste on the biofilms formed by the test microorganisms (Streptococcus mutans, Facklamia homins and Streptococcus saliviurus). The colonizations of the three test bacteria on four surfaces (two natural; permenant and deciduous tooth surfaces and two artificial ;composite and amalgam filling surfaces) were observed with scanning electron microscopy (SEM).SEM detected the anti-biofilm effects on the same four surfaces medicated with a tooth paste. Results: The used tooth paste declined the formed biofilms on four surfaces efficiently. The natural tooth surfaces responded in same manner to the tooth paste while composite responded more efficiently than amlagam surfaces. Conclusions: Flourdated toothpaste (0.454%) effectively can remove formed biofilms of the three test bacteria on natural surfaces and composite filling surfaces. It removed formed biofilm on amalgam surfaces but not as from other three surfaces.

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“…A biofilm is an aggregate of microorganisms embedded within a self-produced matrix of extracellular polymeric substance (EPS). Biofilm EPS is a polymeric conglomeration generally composed of extracellular DNA, proteins, and polysaccharides (Abd El-Baky, 2012).…”

Section: Introductionmentioning

confidence: 99%

Electron Microscopic Assay of Bacterial Biofilm Formed on Indwelling Urethral Catheters

Azmy¹,

Nawar²,

Mohiedden³

et al. 2016

Journal of the Egyptian Society of Parasitology

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Application of Scanning Electron Microscopy for the Morphological Study of Biofilm in Medical Devices

Cited by 5 publications

References 64 publications

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Biofilm formation by Acinetobacter baumannii isolated from medical devices at the intensive care unit of the University Hospital of Tlemcen (Algeria)

Antibiofilm of a Tooth Paste on Cariogenic Bacteria

Electron Microscopic Assay of Bacterial Biofilm Formed on Indwelling Urethral Catheters

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