The effect of sulfide inhibition on the ANAMMOX process

Jin, Ren‐Cun; Yang, Guoguang; Zhang, Qianqian; Ma, Chun; Yu, Jinjin; Xing, Bao-Shan

doi:10.1016/j.watres.2012.12.018

Cited by 228 publications

(55 citation statements)

References 41 publications

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“…This result agrees with a previous study showing that sulfate did not inhibit D. mccartyi FL2 at high concentrations (10 mM) (21). Sulfide exerts inhibitory effects on a variety of cultures with different thresholds (21,(38)(39)(40)(41)(42). A previous study reported that insoluble metal sulfide formation from mg/liter concentrations of heavy metals deactivated sulfate-reducing bacteria by acting as a physical barrier to the cells (35).…”

Section: Discussionsupporting

confidence: 92%

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Mao

Polasko

Alvarez‐Cohen

2017

Appl Environ Microbiol

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In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit the growth or metabolism of pure cultures of the dechlorinator Dehalococcoides mccartyi 195, the sulfate reducer Desulfovibrio vulgaris Hildenborough, or the syntroph Syntrophomonas wolfei. In contrast, sulfide at 5 mM exhibited inhibitory effects on growth of the sulfate reducer and the syntroph, as well as on both dechlorination and growth rates of D. mccartyi. Transcriptomic analysis of D. mccartyi 195 revealed that genes encoding ATP synthase, biosynthesis, and Hym hydrogenase were downregulated during sulfide inhibition, whereas genes encoding metal-containing enzymes involved in energy metabolism were upregulated even though the activity of those enzymes (hydrogenases) was inhibited. When the electron acceptor (trichloroethene) was limiting and an electron donor (lactate) was provided in excess to cocultures and enrichments, high sulfate concentrations (5 mM) inhibited reductive dechlorination due to the toxicity of generated sulfide. The initial cell ratio of sulfate reducers to D. mccartyi (1:3, 1:1, or 3:1) did not affect the dechlorination performance in the presence of sulfate (2 and 5 mM). In contrast, under electron donor limitation, dechlorination was not affected by sulfate amendments due to low sulfide production, demonstrating that D. mccartyi can function effectively in anaerobic microbial communities containing moderate sulfate concentrations (5 mM), likely due to its ability to outcompete other hydrogen-consuming bacteria and archaea.IMPORTANCE Sulfate is common in subsurface environments and has been reported as a cocontaminant with chlorinated solvents at various concentrations. Inconsistent results for the effects of sulfate inhibition on the performance of dechlorination enrichment cultures have been reported in the literature. These inconsistent findings make it difficult to understand potential mechanisms of sulfate inhibition and complicate the interpretation of bioremediation field data. In order to elucidate interactions between sulfate reduction and reductive dechlorination, this study systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. This study provides a more fundamental understanding of the competition mechanisms between reductive dechlorination by Dehalococcoides mccartyi and sulfate reduction during the bioremediation process. It also provides insights on the significance of sulfate concentrations on reductive dechlorination under electron donor/acceptor-limiting conditions during in situ bioremediation applications. For example, at a trichloroethene-contaminated site with a high sulfate concentration, proper...

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Section: Discussionsupporting

confidence: 92%

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Mao

Polasko

Alvarez‐Cohen

2017

Appl Environ Microbiol

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“…The IC 50 of sulfide has been determined using different enrichment cultures of anammox bacteria to be in ranges of 30-300 lM (Carvajal-Arroyo et al, 2013;Dapena-Mora et al, 2007;Jin et al, 2013) whereas IC 50 of ''Ca. Jettenia caeni'' was higher than these values; i.e., 540 lM (Ali et al, 2015a).…”

Section: Ecophysiology and Niche Partitioning Of Anammox Bacteriamentioning

confidence: 99%

Anammox-based technologies for nitrogen removal: Advances in process start-up and remaining issues

2015

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“…Extracellular polymeric substances (EPS) were extracted according to the 'heating' method, and polysaccharide and protein were measured according the anthrone method with a glucose standard and modified Lowry method using bovine serum albumin as a standard according to Ma et al (2012). The particle diameter was determined using a QCOLite image analysis system with a Leica DM2LB microscope and digital camera according to the method described by Jin et al (2013b). The granule settling velocity (V S ) was determined in a measuring cylinder with a height of 40.0 cm and an internal diameter of 10.0 cm to minimize the wall effect.…”

Section: Analytical Methods and Calculationsmentioning

confidence: 99%

Response of performance of an anammox anaerobic baffled reactor (ABR) to changes in feeding regime

Chen

Guo

et al. 2015

Ecological Engineering

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The effect of sulfide inhibition on the ANAMMOX process

Cited by 228 publications

References 41 publications

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Anammox-based technologies for nitrogen removal: Advances in process start-up and remaining issues

Response of performance of an anammox anaerobic baffled reactor (ABR) to changes in feeding regime

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