The effect on the survival of the yeast S. cerevisiae of mixtures of diesel fuel (summer variety) with four representatives of different classes of surfactants – cationic (cetyltrimethylammonium bromide), anionic (sodium dodecyl sulfate), nonionic (Tween-80) and polymeric (polyvinyl alcohol) – was studied. It was shown that diesel fuel and two representatives of the classes of anionic and cationic surfactants (sodium dodecyl sulfate and cetyltrimethylammonium bromide) in the mixture exhibited higher toxicity than when exposed individually. At the same time, the toxicity of diesel fuel mixed with a nonionic surfactant (Tween- 80) and a polymer surfactant (polyvinyl alcohol) was comparable to their individual toxicity.