The effect of susceptible and resistant potato cultivars on bacterial communities in the tuberosphere of potato in soil suppressive or conducive to common scab disease

Abstract

Connections between the structure of bacterial communities in suppressive soils and potato resistance to common scab (CS) are not well understood. In this study, one resistant and one susceptible cultivar were grown in a conducive and suppressive field to assess cultivar resistance × soil suppressiveness interactions. The resistant cultivar had a higher Mg content in periderm compare to susceptible cultivar, while suppressive soil had lower pH (5.3 vs 5.9), N, C, P, Ca contents but higher Fe and S compared with the conducive soil. Bacteria and actinobacteria numbers were higher in the conducive soil. Copy numbers of txtB gene (coding for a pathogenicity determinant) were similar in both soils but were higher in the conducive soil (for periderm samples) and in the susceptible cultivar (for conducive soil samples). Taxonomic microarray analysis and Illumina sequencing of 16S rRNA genes amplicon showed that bacterial community differed between resistant vs susceptible cultivar and to a lesser extend between suppressive vs conducive soil. Bacteria participating in soil suppression belonged to Pseudomonadaceae, Bradyrhizobiaceae, Acetobacteraceae and Paenibacillaceae, while resistant cultivars selected a bacterial community resembling that of the suppressive soil, which was enriched in Nitrospirae and Acidobacteria. Thus, the analysis of soil suppressiveness×cultivar resistance interactions enabled to gain new insight to CS control in the field.IMPORTANCEIt was demonstrated that potato cultivars susceptible and resistant to common scab select differing bacterial community and above that this trait is further modified in suppressive and conducive soil. Common scab severity was diminished by either resistant cultivar or suppressive soil but without additive effect between them. Out of the two factors, potato cultivar had a more significant influence on tuberosphere bacterial community composition than soil. Results highlighted the usefulness of both cultivar resistance and soil suppressiveness traits in understanding and managing disease control of crops.

Differences in Rhizosphere Microbial Community Structure and Composition in Resistance and Susceptible Wheat to Fusarium Head Blight

“…PC1 and PC3 were considered the key factors causing the difference in fungal communities between ZM20 and resistant varieties (Figure 3(b)). Kopecky et al found that both resistant and sensitive species had the differences on the diversity, structure, and composition of soil bacterial communities [32][33][34]. Differences in rhizosphere soil microorganisms of different wheat cultivars may be caused by different plant genotypes, which may affect the composition and abundance of annual plant microbial communities.…”

Streptomyces spp.associadas à sarna da batata

“…Observou-se, em experimentos em casa de vegetação utilizando duas linhagens de Streptomyces não patogênicas contra uma linhagem de S. turgidiscabies, uma total inibição do aparecimento de lesões nos tubérculos (HILTUNEM & KOLLONIEMI 2017). Aplicações repetidas em campo da linhagem de Streptomyces 272 não patogênica resultaram na supressão de sintomas da sarna no decorrer dos anos (KOPECKY et al 2018). Testes empregando diferentes combinações de antagonistas do gênero de Streptomyces em conjunto com ureia resultaram na inibição do patógeno quando comparados com ensaios realizados somente com o antagonista (OT- .…”