The effect of synergism in decreased hydrolysis of biopolymers with enzyme overdosage

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

“…This revealed that an excess of cellulase decreased the enzyme efficiency, analogous to other reports discussing enzyme overdosage (McCrae et al 1989;Marchal et al 1999). This phenomenon occurs frequently in complex (natural) substrate hydrolysis (Markovic et al 2002). We assumed that further increase in total enzyme concentration above the saturation level, and reduced synergistic interactions among components of the crude enzyme, resulted in decreased efficiency of saponin hydrolysis, especially diosgenin production.…”

A wireless magnetoelastic α-amylase sensor

“…When the concentration is more than 275 U/ml, the initial hydrolysis rate is very low. An excess of ␣-amylase decreased the enzyme efficiency, analogously to other articles discussing enzyme overdosage [22][23][24]. The sensor described here can detect 75-125 U/ml ␣-amylase, and 125 U/ml of ␣-amylase was used for the inhibition studies.…”

Determination of uranium in water based on enzyme inhibition using a wireless magnetoelastic sensor

“…When the concentration of -amylase exceeds 150 U mL À1 , the reaction rate hardly increases as the concentration of -amylase increases and the inhibitory effect of uranium decreases consequently. The reason of the reaction rate approaching a plateau above 150 U mL À1 -amylase may be the saturation of the binding sites on the sensor, and also may be that the overdosage of -amylase decreases the enzyme efficiency due to the effect among enzymes discussed in other articles [28,29]. Therefore, in this study, 130 U mL À1 of -amylase was selected for the determination.…”