The effect of temperature on the viability of human mesenchymal stem cells

Reissis, Yannis; García‐Gareta, Elena; Korda, Michelle; Blunn, Gordon; Hua, Jia

doi:10.1186/scrt350

Cited by 45 publications

(23 citation statements)

References 34 publications

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“…Following an additional incubation of 48 hours, its therapeutic efficacy was evaluated. Importantly, our first observation was that mild magnetic hyperthermia alone did not significantly affect AD-MSC viability, which agrees with previously published results on the effects of heat on stem cell viability (Figure 6C) [44]. In terms of its therapeutic efficacy, A2780 ovarian cancer cells treated with conditioned media from the engineered AD-MSCs that were exposed to mild magnetic hyperthermia showed a remarkable decrease in cell viability (40% decrease) when compared to those treated with conditioned media from engineered AD-MSCs controls that had not been exposed to mild magnetic hyperthermia (Figure 6D).…”

Section: Resultssupporting

confidence: 92%

Stem cell-based gene therapy activated using magnetic hyperthermia to enhance the treatment of cancer

et al. 2016

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Stem cell-based gene therapies, wherein stem cells are genetically engineered to express therapeutic molecules, have shown tremendous potential for cancer applications owing to their innate ability to home to tumors. However, traditional stem cell-based gene therapies are hampered by our current inability to control when the therapeutic genes are actually turned on, thereby resulting in detrimental side effects. Here, we report the novel application of magnetic core-shell nanoparticles for the dual purpose of delivering and activating a heat-inducible gene vector that encodes TNF-related apoptosis-inducing ligand (TRAIL) in adipose-derived mesenchymal stem cells (AD-MSCs). By combining the tumor tropism of the AD-MSCs with the spatiotemporal MCNP-based delivery and activation of TRAIL expression, this platform provides an attractive means with which to enhance our control over the activation of stem cell-based gene therapies. In particular, we found that these engineered AD-MSCs retained their innate ability to proliferate, differentiate, and, most importantly, home to tumors, making them ideal cellular carriers. Moreover, exposure of the engineered AD-MSCS to mild magnetic hyperthermia resulted in the selective expression of TRAIL from the engineered AD-MSCs and, as a result, induced significant ovarian cancer cell death in vitro and in vivo.

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Section: Resultssupporting

confidence: 92%

Stem cell-based gene therapy activated using magnetic hyperthermia to enhance the treatment of cancer

et al. 2016

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“…At 12 and 24 h, H2 had significantly higher cell viability, while H5 showed significant lower cell viability compared with NC. This reduced cell viability as temperature increased temperate is in agreement with a previous study on the effect of heat stress on avian cells (Harding et al, 2016), human mesenchymal stromal cells (Reissis et al, 2013), mammalian epithelial cells (Du et al, 2008), and fish hepatic cells (Cui et al, 2013). Our results showed that like viability, the proliferation of CEF was also decreased when cells were maintained at high temperature for 24 h. Higher CEF proliferation was observed in the experimental groups H1, H2, H3, and H4 at 6 and 12 h. The results presented in this study provide the evidence of the beneficial effects of mild heat shock for a short duration on the viability and proliferation of CEF.…”

Section: The Effect Of High Temperature On Viability Proliferation supporting

confidence: 93%

The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells

Ibtisham

Yun-feng

Nawab

et al. 2018

Braz. J. Poult. Sci.

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The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44 o C/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing temperature, but the difference was only significant at 44ºC/6,12h. Heat stress did not significantly affect the superoxide dismutase (SOD) activity. CAT activity was significantly decreased at 43ºC/24h and 44ºC/12 and 24h. Malondialdehyde (MDA) formation was significantly increased at 43ºC/12h and 44ºC/12 and 24h. In conclusion, heat stress induced the oxidative stress, decreasing the viability, proliferation and anti-oxidative response of CEF cells.

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“…Cell viability was assessed by trypan blue exclusion method as described previously (Reissis et al., ). No cell death was observed for different concentrations of SPD‐MAA over a period of 6 to 48 hours.…”

Section: Resultsmentioning

confidence: 99%

Malondialdehyde–Acetaldehyde‐Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A

Sapkota

Kharbanda

Wyatt

2016

Alcoholism Clin & Exp Res

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Background Reactive aldehydes like acetaldehyde and malondialdehyde generated as a result of alcohol metabolism and cigarette smoke exposure lead to the formation of malondialdehyde-acetaldehyde-adducted proteins (MAA adducts). These aldehydes can adduct to different proteins such as bovine serum album (BSA) and surfactant proteins A or D (SPA, SPD). Macrophages play an important role in innate immunity, but the effect of MAA adducts on macrophage function has not yet been examined. Because macrophage scavenger receptor A (SRA; CD204) mediates the uptake of modified proteins, we hypothesized that the effects of MAA modified proteins on macrophage function are primarily mediated through SRA. Methods and Results We tested this hypothesis by exposing SPD-MAA to macrophages and measuring functions. SPD-MAA treatment significantly stimulated pro-inflammatory cytokine TNF-α release in the macrophage cell line, RAW 264.7. A significant reduction in phagocytosis of zymosan particles was also observed. SPD-MAA stimulated a significant dose-dependent increase in TNF-α and IL-6 release from peritoneal macrophages of WT mice. But a significantly less TNF-α and IL-6 were released from peritoneal macrophages of SRA−/− mice. We observed a significant reduction in phagocytosis of zymosan particles in peritoneal macrophages from WT mice treated with SPD-MAA. No further SPD-MAA-induced reduction was seen in peritoneal macrophages form SRA−/− mice. SPD-MAA treatment significantly increased SRA mRNA expression, but had no effect on surface receptor protein expression. Protein kinase C alpha inhibitor and NF-κB inhibitor significantly reduced pro-inflammatory cytokine release in response to SPD-MAA. Conclusion In conclusion, our data demonstrate that SRA is important for MAA-adducted protein-mediated effect on macrophage functions.

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The effect of temperature on the viability of human mesenchymal stem cells

Cited by 45 publications

References 34 publications

Stem cell-based gene therapy activated using magnetic hyperthermia to enhance the treatment of cancer

Stem cell-based gene therapy activated using magnetic hyperthermia to enhance the treatment of cancer

The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells

Malondialdehyde–Acetaldehyde‐Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A

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