The effect of temperature upon the uptake of radioactive phosphate by human erythrocytes in vitro

Gourley, D. R. H.; Gemmill, Chalmers L.

doi:10.1002/jcp.1030350305

Cited by 37 publications

(2 citation statements)

References 12 publications

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“…This value is somewhat higher than 16000 cal./mol. found by Gourley & Gemmill (1950), but both these values are 3-4 times that which would be expected for an exchange process resulting only from passive diffusion.…”

Section: Glycolyticlmentioning

confidence: 68%

A study of the metabolism of phosphorus in mammalian red cells

Prankerd

Altman

1954

Biochemical Journal

158

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were made. All species of spermatozoa tested, including those highly sensitive-to an increase in osmotic pressure, were fully motile in 20M glycerol. It is most unlikely that cells impermeable to glycerol would retain motility in the presence of such an excessive external osmotic pressure, and the retention of motility in the presence of high concentrations of glycerol was taken as evidence that the cells were permeated by this solute. Other neutral solutes can to some extent protect spermatozoa against damage by freezing and thawing (Smith & Polge, 1950), but none is as effective as glycerol. SUMMARY 1. The spermatozoa of the rabbit, the bull, the fowl and the herring were subjected to freezing and thawing in media containing various concentrations of glycerol, and observations made of the resulting changes in motility of the spermatozoa. 2. These observations indicated that the damage suffered by the spermatozoa during freezing and thawing was caused-by their exposure to excessive concentrations of salt when water was removed as ice. 3. The concentration of electrolyte above which irreversible damage occurred was characteristic for the spermatozoa of each species tested. The protective action of glycerol was due to its ability to prevent the salt concentration rising above this level.

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Section: Glycolyticlmentioning

confidence: 68%

A study of the metabolism of phosphorus in mammalian red cells

Prankerd

Altman

1954

Biochemical Journal

158

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“…For example, in red blood cells, the uptake of Pro-labelled phosphate is to a large measure dependent on glycolysis (2). It has a high temperature coefficient (3) and is blocked by metabolic inhibitors (2). Studies of precursor relationships and also of phosphate turnover in red cell stroma suggest that phosphate enters the cell by incorporation in the membrane into glycolytic intermediates such as 2,3-diphosphoglycerate and ATP (4,5).…”

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confidence: 99%

The Active Transport of Phosphate Into the Yeast Cell

Goodman

Rothstein

1957

The Journal of General Physiology

109

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The exchanges of phosphate between cellular and intracellular compartments have been studied in a number of cell types. In each case the entry of phosphate into the cell seems to be associated with metabolic processes at the cell surface (1). For example, in red blood cells, the uptake of Pro-labelled phosphate is to a large measure dependent on glycolysis (2). It has a high temperature coefficient (3) and is blocked by metabolic inhibitors (2). Studies of precursor relationships and also of phosphate turnover in red cell stroma suggest that phosphate enters the cell by incorporation in the membrane into glycolytic intermediates such as 2,3-diphosphoglycerate and ATP (4, 5). In muscle, precursor relationships with 1 ~ also suggest that phosphate enters the cell v/a some phosphorylation reaction (6). However, the latter studies involve the technical difficulty of distinguishing extra-and intracellular orthophosphate in the muscle mass. Autoradiographic studies (7) lend support to the phosphorylation concept. In sea urchin eggs formation of ATP at the cell surface seems to be an integral part of the mechanism of phosphate entry (8).In microorganisms a dependence of phosphate entry on metabolic reactions is well established (9). Yeast has been most intensively studied. In growing yeast, the uptake of PS*-labelled phosphate requires the presence of sugar and is blocked at low temperature (10), and by metabolic inhibitors (9). The phosphate which is absorbed is largely converted to polymerized forms of phosphate (11,12). The total amount of uptake is dependent on the presence of cations such as potassium and magnesium which are also absorbed (13). It is not clear whether the effect of potassium is due to a stimulation of the entry process for phosphate, or to a maintenance of cation-anion balance within the cell, or to stimulation of metaphosphate formation within the cell.In the present study some of the properties of the process of phosphate entry into yeast cells have been investigated, including the kinetics of the reaction, the nature of the substrate, the nature of the entering ion, the effect of potassium, and certain other related phenomena.

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Rates of exchange of phosphate ions in human, rabbit, and chicken blood

Gourley

Matschiner

1953

J. Cell. Comp. Physiol.

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The effect of temperature upon the uptake of radioactive phosphate by human erythrocytes in vitro

Cited by 37 publications

References 12 publications

A study of the metabolism of phosphorus in mammalian red cells

A study of the metabolism of phosphorus in mammalian red cells

The Active Transport of Phosphate Into the Yeast Cell

Rates of exchange of phosphate ions in human, rabbit, and chicken blood

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