The effect of the fluorescent probe, 3,3′-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells

Smith, Thomas C.; Robinson, Susan C.

doi:10.1016/0006-291x(80)90845-1

Cited by 23 publications

(26 citation statements)

References 8 publications

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“…It is well known that some slow response potentiometric indicators affect redox metabolism in mitochondria as well as cell proliferation (364). For example, rhodamine-123 is usually not retained within mitochondria of normal cells when extensively washed, but it is retained within mitochondria of carcinoma and heart muscle cells, resulting in inhibition of their proliferation (29,212,348,380).…”

Section: B Cytotoxicitymentioning

confidence: 99%

Measurement of Intracellular Calcium

Takahashi

Camacho

Lechleiter

et al. 1999

Physiological Reviews

674

511

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To a certain extent, all cellular, physiological, and pathological phenomena that occur in cells are accompanied by ionic changes. The development of techniques allowing the measurement of such ion activities has contributed substantially to our understanding of normal and abnormal cellular function. Digital video microscopy, confocal laser scanning microscopy, and more recently multiphoton microscopy have allowed the precise spatial analysis of intracellular ion activity at the subcellular level in addition to measurement of its concentration. It is well known that Ca2+ regulates numerous physiological cellular phenomena as a second messenger as well as triggering pathological events such as cell injury and death. A number of methods have been developed to measure intracellular Ca2+. In this review, we summarize the advantages and pitfalls of a variety of Ca2+ indicators used in both optical and nonoptical techniques employed for measuring intracellular Ca2+ concentration.

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Section: B Cytotoxicitymentioning

confidence: 99%

Measurement of Intracellular Calcium

Takahashi

Camacho

Lechleiter

et al. 1999

Physiological Reviews

674

511

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“…The latter effect may be less significant quantitatively since very low concentrations of the dye are used. Nonetheless, most investigators using the dyes have recognized some of these problems, such as ATP depletion, by providing alternate energy-yielding pathways, i.e., glycolysis (Philo and Eddy, 1975;Laris et al, 1976;Smith et al, 1981). Correlations of the measurements of the membrane potential by the dye method with other probes (Freedman and Laris, 1981) A major challenge to this conclusion has recently been made by Smith and his collaborators (Smith et al, 1981;Smith and Robinson, 1980;Smith and Robinson, 1981b) with particular reference to the magnitude and responsiveness of the membrane potential in the Ehrlich cell.…”

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confidence: 99%

The use of fluorescent dyes to measure membrane potentials: A critique

Johnstone

Laris

Eddy

1982

Journal Cellular Physiology

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Under controlled conditions, fluorescent cyanine dyes can be used to measure membrane potentials of cell suspensions. Similar changes in membrane potential can be followed both with fluorescent dyes and electrophysiological probes in response to changes in the ion composition of the medium. Recent reports that attempt to abrogate the use of the cyanine dyes in measurements of the membrane potential are misleading.

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“…diS-C3-(5) has been reported to exert toxic effects on Ehrlich ascites tumour cells by rapidly depleting them of ATP in glucose-free medium (Smith et al, 1981). Accordingly, we measured ATP in suspensions of neutrophils as assessed by the chemiluminescent response in the luciferrin-luciferase system (Stanley & Williams, 1969 …”

Section: Membrane Potential Changesmentioning

confidence: 99%

Inhibition of neutrophil activation by p‐bromophenacyl bromide and its effects on phospholipase A₂

Duque

Fantone

Kramer

et al. 1986

British J Pharmacology

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1 In an effort to elucidate the nature ofthe inhibitory effects ofp-bromophenacyl bromide (pBPB) on neutrophil stimulation, we have examined its effects on several stages of stimulus-response coupling. 2 Pretreatment of rat neutrophils with pBPB resulted in a dose-and time-dependent irreversible inhibition of both N-formylmethionyl-leucylphenylalanine (f Met-Leu-Phe)-induced lysosomal enzyme release and change in transmembrane potential. 3 Inhibition of the biological responses to the chemotactic peptide fMet-Leu-Phe was not due to receptor inactivation since fMet-Leu-[3H]-Phe binding to the formyl peptide receptor was not significantly altered by pBPB pretreatment. 4 Inhibition by pBPB of phorbol myristate acetate (PMA)-induced changes in transmembrane potential and the generation of superoxide (02) was also observed. 5 pBPB treatment appeared to inhibit activation of the NADPH oxidase without a direct effect on the oxidase itself. 6 This inhibitory effect was not accompanied by cell death or decrease in cellular titratable sulphydryl groups (at least at doses <20 pM).7 There was, however, significant inhibition of a membranous fraction of fMet-Leu-Phe-induced phospholipase A2 activity by pretreatment with 10 pM pBPB, although total cellular phospholipase A2 was only minimally (<20% inhibition) affected. 8 These data would indicate that pBPB inhibits an early event associated with stimulus-response coupling in rat polymorphonuclear. leukocytes (i.e. change in transmembrane potential). The inhibitory effects of pBPB may be secondary to the inhibition of a critical membranous fraction of cell bound phospholipase A2 activity or its activation, necessary for the initiation of cell activation.

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The effect of the fluorescent probe, 3,3′-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells

Cited by 23 publications

References 8 publications

Measurement of Intracellular Calcium

Measurement of Intracellular Calcium

The use of fluorescent dyes to measure membrane potentials: A critique

Inhibition of neutrophil activation by p‐bromophenacyl bromide and its effects on phospholipase A₂

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The effect of the fluorescent probe, 3,3′-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells

Cited by 23 publications

References 8 publications

Measurement of Intracellular Calcium

Measurement of Intracellular Calcium

The use of fluorescent dyes to measure membrane potentials: A critique

Inhibition of neutrophil activation by p‐bromophenacyl bromide and its effects on phospholipase A2

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Inhibition of neutrophil activation by p‐bromophenacyl bromide and its effects on phospholipase A₂